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iScience2023; 26(2); 106044; doi: 10.1016/j.isci.2023.106044

Species interactions, stability, and resilience of the gut microbiota – Helminth assemblage in horses.

Abstract: The nature and strength of interactions entertained among helminths and their host gut microbiota remain largely unexplored. Using 40 naturally infected Welsh ponies, we tracked the gut microbiota-cyathostomin temporal dynamics and stability before and following anthelmintic treatment and the associated host blood transcriptomic response. High shedders harbored 14 species of cyathostomins, dominated by . They exhibited a highly diverse and temporal dynamic gut microbiota, with butyrate-producing Clostridia likely driving the ecosystem steadiness and host tolerance toward cyathostomins infection. However, anthelmintic administration sharply bent the microbial community. It disrupted the ecosystem stability and the time-dependent network of interactions, affecting longer term microbial resilience. These observations highlight how anthelmintic treatments alter the triangular relationship of parasite, host, and gut microbiota and open new perspectives for adding nutritional intervention to current parasite management strategies.
© 2023 The Author(s).
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Publication Date: 2023-01-25 PubMed ID: 36818309PubMed Central: PMC9929684DOI: 10.1016/j.isci.2023.106044Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates how the interaction between gut bacteria and intestinal worms in horses affects the stability of the gut’s ecosystem, particularly under anthelmintic (anti-parasite) treatment, providing new insights for possible nutrition-based parasite management strategies.

Study Context

  • The researchers aimed to understand the relationships between helminths (intestinal worms), the horse’s gut microbiota (microorganisms including bacteria and fungi), and the impact of anthelmintic treatments on this ecosystem.
  • Previous knowledge of these interactions is scant, so the study helps in filling a gap in the research.

Methodology

  • A total of 40 naturally infected Welsh ponies were included in the study.
  • The research team followed the dynamics and stability of the gut microbiota and cyathostomin (a type of helminth) before and after the administration of an anthelmintic treatment.
  • The researchers also analyzed the responses of the host’s blood transcriptome (the total set of transcripts in a given set of cells).

Findings

  • Ponies with high levels of parasites had 14 species of cyathostomins, primarily dominated by an unspecified species.
  • These high shedders displayed a diverse and temporally dynamic gut microbiota, with Clostridia, a group of bacteria known for butyrate production, possibly driving the stability of the ecosystem and host tolerance to cyathostomin infection.
  • However, the use of anthelmintic treatments considerably changed the bacterial community, disrupting the ecosystem’s stability and the temporal network of interactions, thereby affecting the microbial resilience in the long term.

Implications

  • This study underscores how anthelmintic treatments modify the interplay among the parasite, host, and gut microbiota.
  • The findings present new opportunities for utilizing nutritional interventions alongside traditional parasite management strategies, potentially offering a more holistic and balanced approach for maintaining the health and well-being of horses.

Cite This Article

APA
Boisseau M, Dhorne-Pollet S, Bars-Cortina D, Courtot É, Serreau D, Annonay G, Lluch J, Gesbert A, Reigner F, Sallé G, Mach N. (2023). Species interactions, stability, and resilience of the gut microbiota – Helminth assemblage in horses. iScience, 26(2), 106044. https://doi.org/10.1016/j.isci.2023.106044

Publication

iScience
ISSN: 2589-0042
NlmUniqueID: 101724038
Country: United States
Language: English
Volume: 26
Issue: 2
Pages: 106044
PII: 106044

Researcher Affiliations

Boisseau, Michel
  • , Université de Tours, INRAE, UMR1282 Infectiologie et Santé Publique, 37380 Nouzilly, France.
  • IHAP, Université de Toulouse, INRAE, ENVT, 31076 Toulouse, France.
Dhorne-Pollet, Sophie
  • Université Paris-Saclay, INRAE, AgroParisTech, GABI, 78350 Jouy-en-Josas, France.
Bars-Cortina, David
  • Université Paris-Saclay, INRAE, AgroParisTech, GABI, 78350 Jouy-en-Josas, France.
Courtot, Élise
  • , Université de Tours, INRAE, UMR1282 Infectiologie et Santé Publique, 37380 Nouzilly, France.
Serreau, Delphine
  • , Université de Tours, INRAE, UMR1282 Infectiologie et Santé Publique, 37380 Nouzilly, France.
Annonay, Gwenolah
  • INRAE, US UMR 1426, Genomic platform, 31326 Castanet-Tolosan, France.
Lluch, Jérôme
  • INRAE, US UMR 1426, Genomic platform, 31326 Castanet-Tolosan, France.
Gesbert, Amandine
  • INRAE, UE Physiologie Animale de l'Orfrasière, 37380 Nouzilly, France.
Reigner, Fabrice
  • INRAE, UE Physiologie Animale de l'Orfrasière, 37380 Nouzilly, France.
Sallé, Guillaume
  • , Université de Tours, INRAE, UMR1282 Infectiologie et Santé Publique, 37380 Nouzilly, France.
Mach, Núria
  • Université Paris-Saclay, INRAE, AgroParisTech, GABI, 78350 Jouy-en-Josas, France.
  • IHAP, Université de Toulouse, INRAE, ENVT, 31076 Toulouse, France.

Conflict of Interest Statement

The authors declare no competing interests.

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Citations

This article has been cited 1 times.
  1. Courtot É, Boisseau M, Dhorne-Pollet S, Serreau D, Gesbert A, Reigner F, Basiaga M, Kuzmina T, Lluch J, Annonay G, Kuchly C, Diekmann I, Krücken J, von Samson-Himmelstjerna G, Mach N, Sallé G. Comparison of two molecular barcodes for the study of equine strongylid communities with amplicon sequencing.. PeerJ 2023;11:e15124.
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