Species specificity and interspecies relatedness in VP4 genotypes demonstrated by VP4 sequence analysis of equine, feline, and canine rotavirus strains.
Abstract: We determined the nucleotide and deduced amino acid sequences of the VP4 genes of five equine, two feline, and two canine rotavirus strains. A high degree of homology (> 97.0%) was found among the VP4 amino acid sequences of the equine strains H2, FI-14, and FI23. Equine strain L338 has a distinct VP4 amino acid sequence from those of the other equine strains (78.1% or less homology), and the L338 VP4 exhibited more than 17.0% divergence at the amino acid level from those of rotavirus strains published so far. The VP4 amino acid sequence of equine strain H1, which showed low homology with those of other equine strains, shares > 95.4% homology to those of porcine strains OSU and YM. VP4 amino acid sequences of feline strain Cat97 and canine strains CU-1 and K9 showed a high degree of homology (96.8 to 97.2%) to one another, and were found to be quite similar (96.0-97.0% homology) to that of a human HCR3 strain recently characterized. Feline strain Cat2, whose VP4 sequence is distinct from that of strain Cat97, has a VP4 similar to those of human strains K8 and AU-1 (97.8 and 97.5% homologies at amino acid level, respectively). Thus, the VP4 sequences of rotaviruses showed species specificity and interspecies relatedness.
Publication Date: 1994-05-01 PubMed ID: 8178429DOI: 10.1006/viro.1994.1203Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article examines the genetic similarities of the VP4 gene sequence in equine, feline, and canine rotavirus strains. They found notable degrees of similarity within species and apparent links across different species.
Research Methodology
- The study involved determining the nucleotide and supposed amino acid sequences of the VP4 gene in several strains of rotavirus — specifically five equine, two feline, and two canine strains.
- The researchers looked at the VP4 amino acid sequences and compared their homology, or genetic similarity, within and across species. High homology indicates a significant degree of genetic similarity.
Equine Strains
- The equine strains H2, FI-14, and FI23 had a high degree of homology—over 97% similarity in their VP4 amino acid sequences. This suggests significant genetic similarity among these strains.
- However, equine strain L338 differed notably from the other equine strains, with a maximum of just 78.1% homology with them. This strain’s VP4 had more than 17% divergence at the amino acid level compared to other published rotavirus strains.
- The VP4 amino acid sequence of equine strain H1 was also quite distinguishing among the equine strains. What is notable is its high homology (over 95.4%) with porcine strains OSU and YM, indicating a close genetic correlation.
Feline and Canine Strains
- The feline strain Cat97 and canine strains CU-1 and K9 showed high homology of 96.8 to 97.2% among themselves. They also showed a relatively high homology (96.0-97.0%) when compared to the human HCR3 strain that was recently characterized.
- Feline strain Cat2, whose VP4 sequence differed from that of strain Cat97, shares high homology levels with human strains K8 and AU-1 (97.8% and 97.5%, respectively).
Summary and Implications of Findings
- The research demonstrated species specificity with regard to rotavirus VP4 sequences, highlighting that strains within the same species shared high levels of genetic similarity.
- However, there was also interspecies relatedness, indicating that links exist between rotavirus strains across different species.
- These findings could have significant implications for understanding the transmission dynamics of rotaviruses across different animal and human populations. The ability to identify and trace links across species could potentially inform efforts to manage and control rotavirus outbreaks.
Cite This Article
APA
Taniguchi K, Urasawa T, Urasawa S.
(1994).
Species specificity and interspecies relatedness in VP4 genotypes demonstrated by VP4 sequence analysis of equine, feline, and canine rotavirus strains.
Virology, 200(2), 390-400.
https://doi.org/10.1006/viro.1994.1203 Publication
Researcher Affiliations
- Department of Hygiene, School of Medicine, Sapporo Medical University, Japan.
MeSH Terms
- Amino Acid Sequence
- Animals
- Capsid / genetics
- Capsid Proteins
- Cats / microbiology
- Dogs / microbiology
- Genotype
- Horses / microbiology
- Molecular Sequence Data
- Phylogeny
- Rotavirus / classification
- Rotavirus / genetics
- Sequence Analysis
- Sequence Homology, Amino Acid
- Species Specificity
Citations
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