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Home / Equine Research Bank / Reprod Domest Anim / Spectral Doppler ultrasound in selecting an equine embryo re...
Reproduction in domestic animals = Zuchthygiene2020; 55(6); 747-752; doi: 10.1111/rda.13676

Spectral Doppler ultrasound in selecting an equine embryo receiver.

Abstract: The aim of this study was to evaluate whether the RI and PI values would help in choosing the best embryo recipient, and observe whether CL vascularization would influence P4 production. During the breeding season 2018/2019, the study was conducted using 35 mares, which is used for reference to collect data for the project on the day of embryo transfer. The utilized mares were divided into five groups followed by the day after ovulation, with D0 being the day of ovulation. Therefore, the five groups are as follows: D4-mares that were on the 4th post-ovulation day; D5-mares that were on the 5th post-ovulation day; and doing so successively for D6, D7 and D8. On the day of embryo transfer, the CL of the mares that selected as recipients was evaluated by B-mode and power flow mode ultrasonography and the right and left dorsal branches of the uterine arteries by spectral Doppler ultrasonography. Blood samples were taken on the day of the embryo transfer for a dosage of P4 concentration by radioimmunoassay. No statistical difference was found between the variables when the mares were separated into pregnant and non-pregnant mares, or when they were separated by age groups. When the groups of mares were compared by the day of embryo transfer, the statistical difference was found between the groups D5 × D6 (p = .0053) and D6 × D8 (p = .0036) in RI variable. In PI variable, the statistical difference was found between the groups D4 × D8 (p = .049), D5 × D6 (p = .0446) and D6 × D8 (p = .0024). We conclude that the mares with RI measurement of uterine arteries near 1.0 are correlated to mares with high CL vascularization and elevated P4 concentration.
© 2020 Blackwell Verlag GmbH.
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Publication Date: 2020-04-17 PubMed ID: 32191367DOI: 10.1111/rda.13676Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study focuses on the exploration of using RI (Resistance Index) and PI (Pulsatility Index) values to select the best recipient mare for equine embryo transfer. It further investigates the influence of CL (Corpus Luteum) vascularization on P4 (progesterone) production.

Methodology

  • The study was conducted during the 2018/2019 breeding season, using 35 mares. Data was collected on the day of actual embryo transfer.
  • Mares were divided into five groups based on the number of days post-ovulation: D4 (4th day), D5 (5th day), D6 (6th day), D7 (7th day), and D8 (8th day).
  • Ultrasound examination of the Corpus Luteum of recipient mares was done on the day of embryo transfer. This non-invasive imaging technique allowed the researchers to visualize blood flow.
  • Spectral Doppler ultrasonography was used to evaluate the uterine arteries. This technique provided information about blood velocity and blood flow resistance in the vessels.
  • Progesterone, a hormone essential for maintaining pregnancy, was measured through blood samples taken on the day of embryo transfer.

Findings

  • Neither the mares’ pregnancy status nor their age groups showed any statistical difference in variables like RI, PI, and P4.
  • However, when mares were grouped based on the day of embryo transfer, the study found statistical differences in RI values between D5 and D6 (p = .0053), and between D6 and D8 (p = .0036).
  • Significant variations were also noted in PI values between D4 and D8 (p = .049), D5 and D6 (p = .0446), and D6 and D8 (p = .0024).

Conclusion

  • The mares with RI measurements of uterine arteries near 1.0 were aligned with mares exhibiting high CL vascularization and elevated P4 concentrations. This suggests that these parameters may provide useful insights in identifying suitable recipients for equine embryo transfer.

Cite This Article

APA
Ferreira CSC, Morais RCL, de Andrade ABP, Balaro MFA, Ribas JAS, Gomes GM, Pinna AE. (2020). Spectral Doppler ultrasound in selecting an equine embryo receiver. Reprod Domest Anim, 55(6), 747-752. https://doi.org/10.1111/rda.13676

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 55
Issue: 6
Pages: 747-752

Researcher Affiliations

Ferreira, Camila Silva Costa
  • Fluminense Federal University-UFF, Niterói, Brazil.
Morais, Rita de Cássia Lima
  • Rural Federal University of Rio de Janeiro-UFRRJ, Seropédica, Brazil.
de Andrade, Amanda Bricio Pereira
  • Fluminense Federal University-UFF, Niterói, Brazil.
Balaro, Mário Felipe A
  • Fluminense Federal University-UFF, Niterói, Brazil.
Ribas, José Antônio Silva
  • Fluminense Federal University-UFF, Niterói, Brazil.
Gomes, Gustavo Mendes
  • Vassouras University, Vassouras, Brazil.
Pinna, Aline Emerim
  • Fluminense Federal University-UFF, Niterói, Brazil.

MeSH Terms

  • Animals
  • Corpus Luteum / blood supply
  • Corpus Luteum / diagnostic imaging
  • Embryo Transfer / veterinary
  • Embryo, Mammalian
  • Female
  • Horses / physiology
  • Pregnancy
  • Progesterone / blood
  • Ultrasonography, Doppler / veterinary
  • Uterine Artery / diagnostic imaging

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