Sperm morphology in stallions: ultrastructure as a functional and diagnostic tool.
- Journal Article
- Review
Summary
This research paper presents a detailed diagnostic method for assessing the functional impairment of sperm cells in stallions. It suggests that the scrutinizing of specific structural components at a microscopic level, in conjunction with a qualification of the spermatozoa organ at an ultrastructural level, is a more affordable and efficient way to evaluate sperm function.
Background
In stallions’ reproductive biology, an robust evaluation method for sperm is vital to assess the ability of stallions to reproduce. Conventional examinations typically use light microscopy, however, the authors argue that this method is not sufficient to identify potential indicators of functional impairment in sperm cells.
- The study proposes an advanced technique that involves critical quantitative evaluation of the morphology of individual spermatozoa components at the microscopic level and critical qualitative examination of sperm cell organelles at the ultrastructural level.
Method
This technique enables a more nuanced understanding of sperm function, going beyond what’s possible with simple light microscopic evaluation.
- The approach is comparative to a range of sperm function tests used in human andrology clinics.
Implications and Conclusions
The advanced diagnostic technique proposed by the authors offers several potential advantages over existing methods. This new method is efficient, cost-effective, simple, and at the same time comprehensive in assessing sperm function.
- This technique could be a beneficial diagnostic tool in evaluating stallion’s fertility by providing a fuller understanding of the functional capacity of sperm cells.
- The study also implies the potential application of these diagnostic methods to other species, broadening the scope beyond just stallions.
Cite This Article
Publication
Researcher Affiliations
- Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523-1683, USA. d_n_rao.veeramachaneni@colostate.edu
MeSH Terms
- Animals
- Chromatin / ultrastructure
- Fertility
- Horse Diseases / pathology
- Horses / anatomy & histology
- Horses / physiology
- Infertility, Male / pathology
- Infertility, Male / veterinary
- Male
- Microscopy, Electron, Transmission / veterinary
- Mitochondria / ultrastructure
- Semen / cytology
- Seminiferous Epithelium / ultrastructure
- Sertoli Cells / ultrastructure
- Sperm Agglutination / physiology
- Spermatozoa / abnormalities
- Spermatozoa / ultrastructure
- Tissue Fixation / methods
- Tissue Fixation / veterinary
Citations
This article has been cited 6 times.- Orsolini MF, Meyers SA, Dini P. An Update on Semen Physiology, Technologies, and Selection Techniques for the Advancement of In Vitro Equine Embryo Production: Section I. Animals (Basel) 2021 Nov 13;11(11).
- Agarwal A, Cho CL, Esteves SC, Majzoub A. All-round approach in diagnosis. Transl Androl Urol 2017 Sep;6(Suppl 4):S465-S467.
- Veeramachaneni DNR. Utility of testing sperm DNA fragmentation: an all-in-one diagnostic tool to address a multi-pronged clinical problem. Transl Androl Urol 2017 Sep;6(Suppl 4):S462-S464.
- Phetudomsinsuk K, Sirinarumitr K, Laikul A, Pinyopummin A. Morphology and head morphometric characters of sperm in Thai native crossbred stallions. Acta Vet Scand 2008 Oct 22;50(1):41.
- Veeramachaneni DN. Impact of environmental pollutants on the male: effects on germ cell differentiation. Anim Reprod Sci 2008 Apr;105(1-2):144-57.
- Waqas MS, Arroyo E, Tibary A. Diagnostic Approach to Equine Testicular Disorders. Vet Sci 2024 May 29;11(6).