Sperm selection using single layer centrifugation prior to cryopreservation can increase thawed sperm quality in stallions.
Abstract: The increasing use of modern reproductive techniques in human medicine has led to a higher demand for isolation of motile sperm. Several of these isolation techniques have been adapted for veterinary use and can be applied for the selection of a superior sperm sample from stallion semen. Until recently a major disadvantage of such isolation techniques was the limitation in sperm volume that could be handled. Androcoll-E had been shown to be successful for processing large volumes of equine semen but there are few data to substantiate the potential beneficial effect of freezing an Androcoll-E selected equine sperm sample to obtain higher quality following thawing. Objective: In this study, the effect of Androcoll-E treatment of sperm prior to cryopreservation was compared with cushioned centrifugation using ejaculates from 8 different stallions selected because they were known to have semen of differing quality following freezing. Results: Androcoll-E treatment increased measures of semen quality prior to freezing. However, Androcoll-E treatment reduced the yield of sperm following centrifugation when compared with the cushion centrifuged control group (50.9 ± 14.2% vs. 97.1 ± 9.0%, respectively). Quality analysis following thawing showed an overall improved sperm quality for Androcoll-E treated samples and average post thaw progressive motility (PM) was 41.6% compared with 30.5% for the cushion centrifuged group. Conclusions: Androcoll-E can be used with good results to select a superior sperm population prior to cryopreservation, in order to produce good-quality frozen thawed semen.
© 2011 EVJ Ltd.
Publication Date: 2011-12-07 PubMed ID: 22082444DOI: 10.1111/j.2042-3306.2011.00489.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigates how sperm selection using a technique called single layer centrifugation with Androcoll-E before freezing can lead to a greater quality of thawed sperm in stallions.
Methods and Objective
- The researchers used Androcoll-E, a sperm isolation technique, to treat sperm from eight different stallions selected for their differing quality of semen after freezing.
- The primary objective of the study was to compare the effect of pre-cryopreservation treatment with Androcoll-E versus cushioned centrifugation.
Results
- The researchers found that treating the semen with Androcoll-E increased measures of semen quality before freezing compared to the conventional cushioned centrifugation technique.
- However, they also noted that the overall yield of sperm following centrifugation was lower with the Androcoll-E treatment compared to the control group – about half.
- The post-thaw quality analysis showed a significant improvement in sperm quality for Androcoll-E treated samples. The average post-thaw progressive motility (the ability of the sperm to move efficiently towards an egg for fertilization) was found to be 41.6% for the Androcoll-E group, compared with 30.5% for the cushion centrifuged group.
Conclusions
- The authors concluded that Androcoll-E effectively selects superior sperm populations prior to freezing, leading to higher quality thawed semen.
- This suggests that using Androcoll-E for sperm selection prior to cryopreservation could be beneficial in enhancing reproduction effectiveness in stallions, despite the somewhat lower yield.
Implications
- While the study was conducted on stallion semen, the results could potentially be applied to other species and may have implications for human reproductive medicine given the similarities in sperm cryopreservation procedures.
- Further research would be needed to validate these findings across different species and to address the lower yield issue.
Cite This Article
APA
Hoogewijs M, Morrell J, Van Soom A, Govaere J, Johannisson A, Piepers S, De Schauwer C, De Kruif A, De Vliegher S.
(2011).
Sperm selection using single layer centrifugation prior to cryopreservation can increase thawed sperm quality in stallions.
Equine Vet J Suppl(40), 35-41.
https://doi.org/10.1111/j.2042-3306.2011.00489.x Publication
Researcher Affiliations
- Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Belgium. maarten.hoogewijs@UGent.be
MeSH Terms
- Animals
- Centrifugation / methods
- Centrifugation / veterinary
- Cryopreservation / methods
- Cryopreservation / veterinary
- Horses / physiology
- Male
- Semen Analysis / veterinary
- Semen Preservation / methods
- Semen Preservation / veterinary
- Spermatozoa / physiology
Citations
This article has been cited 6 times.- Gutiérrez-Cepeda L, Crespo F, Blazquez JC, Serres C. Optimization of the Equine-Sperm Freeze Test in Purebred Spanish Horses by Incorporating Colloidal Centrifugation.. Animals (Basel) 2023 Jan 22;13(3).
- Domain G, Ali Hassan H, Wydooghe E, Bogado Pascottini O, Johannisson A, Morrell JM, Niżański W, Van Soom A. Influence of Single Layer Centrifugation with Canicoll on Semen Freezability in Dogs.. Animals (Basel) 2022 Mar 11;12(6).
- Zuidema D, Kerns K, Sutovsky P. An Exploration of Current and Perspective Semen Analysis and Sperm Selection for Livestock Artificial Insemination.. Animals (Basel) 2021 Dec 15;11(12).
- Guillén Palomino CY, Fumuso FG, Bertuzzi ML, Giuliano SM, Velásquez González N, Bariani MV, Carretero MI. Use of Androcoll-E(TM) to Separate Frozen-Thawed Llama Sperm From Seminal Plasma and Diluent.. Front Vet Sci 2020;7:594926.
- Gloria A, Carluccio A, Wegher L, Robbe D, Befacchia G, Contri A. Single and double layer centrifugation improve the quality of cryopreserved bovine sperm from poor quality ejaculates.. J Anim Sci Biotechnol 2016;7:30.
- Gutiérrez-Cepeda L, Fernández A, Crespo F, Ramírez MÁ, Gosálvez J, Serres C. The effect of two pre-cryopreservation single layer colloidal centrifugation protocols in combination with different freezing extenders on the fragmentation dynamics of thawed equine sperm DNA.. Acta Vet Scand 2012 Dec 5;54(1):72.
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