FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Experientia1995; 51(8); 804-808; doi: 10.1007/BF01922434

Sr2+ can become incorporated into an agonist-sensitive, cytoplasmic Ca2+ store in a cell line derived from the equine sweat gland epithelium.

Abstract: We have explored the properties of a Ca(2+)-dependent cell-signalling pathway that becomes active when cultured equine sweat gland cells are stimulated with ATP. The ATP-regulated, Ca(2+)-influx pathway allowed Sr2+ to enter the cytoplasm but permitted only a minimal influx of Ba2+. Experiments in which cells were repeatedly stimulated with ATP suggested that Sr2+, but not Ba2+, could become incorporated into the agonist-sensitive, cytoplasmic Ca2+ store. Further evidence for this was provided by experiments using ionomycin, a Ca2+ ionophore which has no affinity for Sr2+.
Get Full Text
Publication Date: 1995-08-16 PubMed ID: 7649240DOI: 10.1007/BF01922434Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores how Strontium (Sr2+) can be integrated into an ATP-sensitive calcium (Ca2+) store in sweat gland cells from horses. The study involved experiments on cultured cells, evaluating how the influx of Sr2+ and Barium (Ba2+) are controlled, and unveiling that only Sr2+ could be incorporated into the cytoplasmic Ca2+ store, a key element in cell signaling.

Study of Ca(2+)-Dependent Cell-Signalling Pathway

  • The researchers focused on a specific cell-signaling pathway in cultured equine sweat gland cells that can be activated with Adenosine Triphosphate (ATP).
  • ATP is a primary molecule that carries energy within cells and thus plays a crucial role in managing a range of physiological processes in the cells.

Investigating Role of Sr2+ and Ba2+

  • The research discovered that the ATP-controlled Ca(2+) influx pathway allowed Sr2+ to enter the cytoplasm, whereas the influx of Ba2+ was minimal.
  • Influx pathway describes the mechanisms by which certain ions or molecules enter a cell.
  • It’s also suggested Sr2+, unlike Ba2+, could be incorporated into what the researchers term the ‘agonist-sensitive, cytoplasmic Ca2+ store’.
  • The ‘agonist-sensitive, cytoplasmic Ca2+ store’ could refer to a pool or storage of Ca2+ ion regulated by ATP within the cell’s cytoplasm which is ready to be used when needed or upon stimulation.

Ionomycin Experiment

  • Additional evidence to back the theory that Sr2+ could be incorporated into the cytoplasmic Ca2+ store was provided by experiments involving ionomycin.
  • Ionomycin is a type of calcium ionophore – substances which are able to form a structure that can shuttle ions across the cell membrane.
  • In this context, ionomycin provided a way of verifying that Sr2+ was indeed being integrated into the intracellular store since ionomycin has no affinity for Sr2+, indicating that if Sr2+ was present, it must have used another pathway to enter the Ca2+ storage site.

Cite This Article

APA
Ko WH, Pediani JD, Bovell DL, Wilson SM. (1995). Sr2+ can become incorporated into an agonist-sensitive, cytoplasmic Ca2+ store in a cell line derived from the equine sweat gland epithelium. Experientia, 51(8), 804-808. https://doi.org/10.1007/BF01922434

Publication

Experientia
ISSN: 0014-4754
NlmUniqueID: 0376547
Country: Switzerland
Language: English
Volume: 51
Issue: 8
Pages: 804-808

Researcher Affiliations

Ko, W H
  • Division of Neuroscience and Biomedical Systems, University of Glasgow, Scotland, UK.
Pediani, J D
    Bovell, D L
      Wilson, S M

        MeSH Terms

        • Adenosine Triphosphate / metabolism
        • Animals
        • Barium / metabolism
        • Calcium / metabolism
        • Cell Line
        • Cytoplasm / metabolism
        • Epithelium
        • Fura-2
        • Horses
        • In Vitro Techniques
        • Receptors, Purinergic / physiology
        • Strontium / metabolism
        • Sweat Glands / cytology
        • Sweat Glands / metabolism

        References

        This article includes 19 references
        1. Somlyo AP, Somlyo AV, Devine CE, Peters PD, Hall TA. Electron microscopy and electron probe analysis of mitochondrial cation accumulation in smooth muscle.. J Cell Biol 1974 Jun;61(3):723-42.
          pubmed: 4836390doi: 10.1083/jcb.61.3.723google scholar: lookup
        2. Yamaguchi DT, Green J, Kleeman CR, Muallem S. Properties of the depolarization-activated calcium and barium entry in osteoblast-like cells.. J Biol Chem 1989 Jan 5;264(1):197-204.
          pubmed: 2491847
        3. Gill DL, Grollman EF, Kohn LD. Calcium transport mechanisms in membrane vesicles from guinea pig brain synaptosomes.. J Biol Chem 1981 Jan 10;256(1):184-92.
          pubmed: 6778859
        4. Takemura H, Hughes AR, Thastrup O, Putney JW Jr. Activation of calcium entry by the tumor promoter thapsigargin in parotid acinar cells. Evidence that an intracellular calcium pool and not an inositol phosphate regulates calcium fluxes at the plasma membrane.. J Biol Chem 1989 Jul 25;264(21):12266-71.
          pubmed: 2663854
        5. Merritt JE, Hallam TJ. Platelets and parotid acinar cells have different mechanisms for agonist-stimulated divalent cation entry.. J Biol Chem 1988 May 5;263(13):6161-4.
          pubmed: 3360779
        6. Petersen OH. Stimulus-secretion coupling: cytoplasmic calcium signals and the control of ion channels in exocrine acinar cells.. J Physiol 1992 Mar;448:1-51.
          pubmed: 1375633doi: 10.1113/jphysiol.1992.sp019028google scholar: lookup
        7. Stuenkel EL, Ernst SA. Multiple calcium mobilization pathways in single avian salt gland cells.. Am J Physiol 1990 Feb;258(2 Pt 1):C289-98.
          pubmed: 1689544doi: 10.1152/ajpcell.1990.258.2.C289google scholar: lookup
        8. Leung AY, Tai HL, Wong PY. ATP stimulates Ca2+ release from a rapidly exchanging pool in cultured rat epididymal cells.. Am J Physiol 1993 Jun;264(6 Pt 1):C1388-94.
          pubmed: 8333493doi: 10.1152/ajpcell.1993.264.6.C1388google scholar: lookup
        9. Bijman J, Quinton PM. Predominantly beta-adrenergic control of equine sweating.. Am J Physiol 1984 Mar;246(3 Pt 2):R349-53.
          pubmed: 6322597doi: 10.1152/ajpregu.1984.246.3.R349google scholar: lookup
        10. Liu C, Hermann TE. Characterization of ionomycin as a calcium ionophore.. J Biol Chem 1978 Sep 10;253(17):5892-4.
          pubmed: 28319
        11. Kwan CY, Putney JW Jr. Uptake and intracellular sequestration of divalent cations in resting and methacholine-stimulated mouse lacrimal acinar cells. Dissociation by Sr2+ and Ba2+ of agonist-stimulated divalent cation entry from the refilling of the agonist-sensitive intracellular pool.. J Biol Chem 1990 Jan 15;265(2):678-84.
          pubmed: 2404009
        12. Thastrup O, Cullen PJ, Drøbak BK, Hanley MR, Dawson AP. Thapsigargin, a tumor promoter, discharges intracellular Ca2+ stores by specific inhibition of the endoplasmic reticulum Ca2(+)-ATPase.. Proc Natl Acad Sci U S A 1990 Apr;87(7):2466-70.
          pubmed: 2138778doi: 10.1073/pnas.87.7.2466google scholar: lookup
        13. Kwan CY, Takemura H, Obie JF, Thastrup O, Putney JW Jr. Effects of MeCh, thapsigargin, and La3+ on plasmalemmal and intracellular Ca2+ transport in lacrimal acinar cells.. Am J Physiol 1990 Jun;258(6 Pt 1):C1006-15.
          pubmed: 2360617doi: 10.1152/ajpcell.1990.258.6.C1006google scholar: lookup
        14. Bijman J, Quinton PM. Influence of calcium and cyclic nucleotides on beta-adrenergic sweat secretion in equine sweat glands.. Am J Physiol 1984 Jul;247(1 Pt 1):C10-3.
          pubmed: 6331181doi: 10.1152/ajpcell.1984.247.1.C10google scholar: lookup
        15. Gill DL, Chueh SH. An intracellular (ATP + Mg2+)-dependent calcium pump within the N1E-115 neuronal cell line.. J Biol Chem 1985 Aug 5;260(16):9289-97.
          pubmed: 3160697
        16. Grynkiewicz G, Poenie M, Tsien RY. A new generation of Ca2+ indicators with greatly improved fluorescence properties.. J Biol Chem 1985 Mar 25;260(6):3440-50.
          pubmed: 3838314
        17. Dubyak GR, el-Moatassim C. Signal transduction via P2-purinergic receptors for extracellular ATP and other nucleotides.. Am J Physiol 1993 Sep;265(3 Pt 1):C577-606.
          pubmed: 8214015doi: 10.1152/ajpcell.1993.265.3.C577google scholar: lookup
        18. Schilling WP, Rajan L, Strobl-Jager E. Characterization of the bradykinin-stimulated calcium influx pathway of cultured vascular endothelial cells. Saturability, selectivity, and kinetics.. J Biol Chem 1989 Aug 5;264(22):12838-48.
          pubmed: 2546937
        19. Berridge MJ. Inositol trisphosphate and calcium signalling.. Nature 1993 Jan 28;361(6410):315-25.
          pubmed: 8381210doi: 10.1038/361315a0google scholar: lookup

        Citations

        This article has been cited 1 times.
        1. Ko WH, Chan HC, Wong PY. Anion secretion induced by capacitative Ca2+ entry through apical and basolateral membranes of cultured equine sweat gland epithelium. J Physiol 1996 Nov 15;497 ( Pt 1)(Pt 1):19-29.
          doi: 10.1113/jphysiol.1996.sp021746pubmed: 8951708google scholar: lookup
        Subscribe to our newsletter
        Join 100,151 horse owners like you who receive our email updates on horse health and nutrition.