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Home / Equine Research Bank / Experientia / Sr2+ can become incorporated into an agonist-sensitive, cyto...
Experientia1995; 51(8); 804-808; doi: 10.1007/BF01922434

Sr2+ can become incorporated into an agonist-sensitive, cytoplasmic Ca2+ store in a cell line derived from the equine sweat gland epithelium.

Abstract: We have explored the properties of a Ca(2+)-dependent cell-signalling pathway that becomes active when cultured equine sweat gland cells are stimulated with ATP. The ATP-regulated, Ca(2+)-influx pathway allowed Sr2+ to enter the cytoplasm but permitted only a minimal influx of Ba2+. Experiments in which cells were repeatedly stimulated with ATP suggested that Sr2+, but not Ba2+, could become incorporated into the agonist-sensitive, cytoplasmic Ca2+ store. Further evidence for this was provided by experiments using ionomycin, a Ca2+ ionophore which has no affinity for Sr2+.
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Publication Date: 1995-08-16 PubMed ID: 7649240DOI: 10.1007/BF01922434Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores how Strontium (Sr2+) can be integrated into an ATP-sensitive calcium (Ca2+) store in sweat gland cells from horses. The study involved experiments on cultured cells, evaluating how the influx of Sr2+ and Barium (Ba2+) are controlled, and unveiling that only Sr2+ could be incorporated into the cytoplasmic Ca2+ store, a key element in cell signaling.

Study of Ca(2+)-Dependent Cell-Signalling Pathway

  • The researchers focused on a specific cell-signaling pathway in cultured equine sweat gland cells that can be activated with Adenosine Triphosphate (ATP).
  • ATP is a primary molecule that carries energy within cells and thus plays a crucial role in managing a range of physiological processes in the cells.

Investigating Role of Sr2+ and Ba2+

  • The research discovered that the ATP-controlled Ca(2+) influx pathway allowed Sr2+ to enter the cytoplasm, whereas the influx of Ba2+ was minimal.
  • Influx pathway describes the mechanisms by which certain ions or molecules enter a cell.
  • It’s also suggested Sr2+, unlike Ba2+, could be incorporated into what the researchers term the ‘agonist-sensitive, cytoplasmic Ca2+ store’.
  • The ‘agonist-sensitive, cytoplasmic Ca2+ store’ could refer to a pool or storage of Ca2+ ion regulated by ATP within the cell’s cytoplasm which is ready to be used when needed or upon stimulation.

Ionomycin Experiment

  • Additional evidence to back the theory that Sr2+ could be incorporated into the cytoplasmic Ca2+ store was provided by experiments involving ionomycin.
  • Ionomycin is a type of calcium ionophore – substances which are able to form a structure that can shuttle ions across the cell membrane.
  • In this context, ionomycin provided a way of verifying that Sr2+ was indeed being integrated into the intracellular store since ionomycin has no affinity for Sr2+, indicating that if Sr2+ was present, it must have used another pathway to enter the Ca2+ storage site.

Cite This Article

APA
Ko WH, Pediani JD, Bovell DL, Wilson SM. (1995). Sr2+ can become incorporated into an agonist-sensitive, cytoplasmic Ca2+ store in a cell line derived from the equine sweat gland epithelium. Experientia, 51(8), 804-808. https://doi.org/10.1007/BF01922434

Publication

Experientia
ISSN: 0014-4754
NlmUniqueID: 0376547
Country: Switzerland
Language: English
Volume: 51
Issue: 8
Pages: 804-808

Researcher Affiliations

Ko, W H
  • Division of Neuroscience and Biomedical Systems, University of Glasgow, Scotland, UK.
Pediani, J D
    Bovell, D L
      Wilson, S M

        MeSH Terms

        • Adenosine Triphosphate / metabolism
        • Animals
        • Barium / metabolism
        • Calcium / metabolism
        • Cell Line
        • Cytoplasm / metabolism
        • Epithelium
        • Fura-2
        • Horses
        • In Vitro Techniques
        • Receptors, Purinergic / physiology
        • Strontium / metabolism
        • Sweat Glands / cytology
        • Sweat Glands / metabolism

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        Citations

        This article has been cited 1 times.
        1. Ko WH, Chan HC, Wong PY. Anion secretion induced by capacitative Ca2+ entry through apical and basolateral membranes of cultured equine sweat gland epithelium. J Physiol 1996 Nov 15;497 ( Pt 1)(Pt 1):19-29.
          doi: 10.1113/jphysiol.1996.sp021746pubmed: 8951708google scholar: lookup
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