Stallion spermatozoa selected by single layer centrifugation are capable of fertilization after storage for up to 96 h at 6°C prior to artificial insemination.

This research paper investigates whether stallion sperm, selected using Single Layer Centrifugation and stored at low temperatures, retains its ability to fertilize after prolonged storages of up to 96 hours.

Objective of the Research

• The aim of this study was to test if SLC-selected stallion spermatozoa could continue to perform successful fertilization after long periods of storage at low temperatures. This investigation aimed to assist equine breeders who encounter the challenge of providing high-quality semen stimuli for artificial insemination at the precise time a mare is ready for ovulation.

Methodology and Process

• Single Layer Centrifugation (SLC) was utilized for the selection of the stallion spermatozoa. This method has previously been identified for effectively choosing spermatozoa with intact chromatin, good progressive motility, and normal morphology from the ejaculate and for prolonging the selected spermatozoa’s life in vitro.
• SLC-selected stallion sperm doses that had been cooled and stored at 6°C for 48 hours, 72 hours, or 96 hours were used to inseminate sixteen mares.
• Post presumed ovulation, an ultrasound examination was carried out 16 to 18 days later, during which embryos were identified in 11 mares.

Results and Conclusion

• The study’s results confirmed that SLC-selected stallion spermatozoa, preserved for extended periods of up to 96 hours at 6°C, maintained their ability to fertilize. Therefore, this outcome has positive implications for equine breeders, as it allows for greater flexibility in timing inseminations based on when the mare is due to ovulate and the potential to increase successful fertilizations.