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Home / Equine Research Bank / Vet J / Storage of equine red blood cells as a concentrate.
Veterinary journal (London, England : 1997)2007; 176(2); 227-231; doi: 10.1016/j.tvjl.2007.02.015

Storage of equine red blood cells as a concentrate.

Abstract: The study was undertaken to determine how equine red blood cells (RBCs) survive in storage bags designed for use with human RBCs. Separated RBCs were stored in a routine manner for 35 days and examined every 7 days for storage lesions. Measured parameters included haematology, haemolysis, pH, potassium, lactate, adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (2,3-DPG). All tests were performed in vitro. Haematology did not change significantly. Haemolysis increased during storage but did not exceed human limits. pH and 2,3-DPG decreased, while lactate, potassium and ATP increased. RBCs deteriorated somewhat during storage, but when compared with human in vitro parameters, remained suitable for transfusion. It is concluded that equine erythrocytes can be stored for at least 35 days before transfusion.
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Publication Date: 2007-04-20 PubMed ID: 17449302DOI: 10.1016/j.tvjl.2007.02.015Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article studied the viability of storing horse red blood cells (RBCs) in human blood storage bags and concluded that they can be stored safely for at least 35 days.

Objective of the Research

  • The main aim of the research was to investigate how horse red blood cells (RBCs) would fare when stored in blood bags that are typically employed for human RBCs’ storage.

Methodology

  • The procedure involved the storage of separated horse RBCs in a standard way for a period of 35 days.
  • Every 7 days, the cells were examined for storage lesions or changes that could occur through their interaction with the storage bags.
  • The parameters measured included the RBC’s hematological aspects, hemolysis (the breaking down of red blood cells), pH level, concentrations of potassium, lactate, adenosine triphosphate (ATP), and 2,3-diphosphoglycerate (2,3-DPG). All examinations were performed in a controlled, in vitro (lab, outside of a living organism) environment.

Findings

  • The research found that the hematological aspects of the stored horse RBCs did not significantly alter.
  • Hemolysis increased as the storage period progressed but did not exceed the safe limits set for human blood storage.
  • While levels of pH and 2,3-DPG decreased over the storage period, concentrations of lactate, potassium, and ATP were found to have elevated.
  • RBCs underwent some level of deterioration in the storage bags but, when compared to human RBCs stored under the in vitro conditions, the equine RBCs were still deemed appropriate for transfusion.

Conclusion

  • Based on the observations drawn from the study, it was inferred that horse red blood cells can be securely stored in human-designated blood bags for at least 35 days before being used for transfusion.

Cite This Article

APA
Niinistö K, Raekallio M, Sankari S. (2007). Storage of equine red blood cells as a concentrate. Vet J, 176(2), 227-231. https://doi.org/10.1016/j.tvjl.2007.02.015

Publication

Veterinary journal (London, England : 1997)
ISSN: 1090-0233
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 176
Issue: 2
Pages: 227-231

Researcher Affiliations

Niinistö, Kati
  • Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57, 00014 Helsinki, Finland. kati.niinisto@fimnet.fi
Raekallio, Marja
    Sankari, Satu

      MeSH Terms

      • 2,3-Diphosphoglycerate / blood
      • Adenosine Triphosphate / blood
      • Animals
      • Blood Preservation / methods
      • Blood Preservation / veterinary
      • Erythrocytes / chemistry
      • Hemolysis
      • Horses / blood
      • Hydrogen-Ion Concentration
      • Lactic Acid / blood
      • Potassium / blood

      Citations

      This article has been cited 10 times.
      1. Miglio A, Cremonini V, Leonardi L, Manuali E, Coliolo P, Barbato O, Dall'Aglio C, Antognoni MT. Omics Technologies in Veterinary Medicine: Literature Review and Perspectives in Transfusion Medicine. Transfus Med Hemother 2023 Jun;50(3):198-207.
        doi: 10.1159/000530870pubmed: 37408648google scholar: lookup
      2. Zhong R, Wu X, Liu Z, He Z, Zhang X, Liu J, Cao Y, Wang H. A study on the correlation between hemoglobin concentration and the storage quality of suspended red blood cells prepared from the whole blood of Tibetan male residents. Front Med (Lausanne) 2022;9:1062778.
        doi: 10.3389/fmed.2022.1062778pubmed: 36743680google scholar: lookup
      3. Jamieson CA, Baillie SL, Johnson JP. Blood Transfusion in Equids-A Practical Approach and Review. Animals (Basel) 2022 Aug 23;12(17).
        doi: 10.3390/ani12172162pubmed: 36077883google scholar: lookup
      4. Barros IO, Sousa RS, Tavares MD, Rêgo RO, Firmino PR, Souza FJA, Abrantes MR, Minervino AHH, Araújo CASC, Ortolani EL, Barrêto Júnior RA. Assessment of Donkey (Equus asinus africanus) Whole Blood Stored in CPDA-1 and CPD/SAG-M Blood Bags. Biology (Basel) 2021 Feb 8;10(2).
        doi: 10.3390/biology10020133pubmed: 33567685google scholar: lookup
      5. Zhong R, Liu H, Wang H, Li X, He Z, Gangla M, Zhang J, Han D, Liu J. Adaption to High Altitude: An Evaluation of the Storage Quality of Suspended Red Blood Cells Prepared from the Whole Blood of Tibetan Plateau Migrants. PLoS One 2015;10(12):e0144201.
        doi: 10.1371/journal.pone.0144201pubmed: 26637115google scholar: lookup
      6. Fallahi L, Ghiliyan R, Hashemi A, Fatemi A, Saeedi M. Comparing Electrolytes in Prestorage Leukocyte-Reduced Packed Cell versus Unfiltered Packed Cell. Iran J Ped Hematol Oncol 2013;3(3):86-90.
        pubmed: 24575277
      7. Ran Q, Hao P, Xiao Y, Zhao J, Ye X, Li Z. Effect of irradiation and/or leucocyte filtration on RBC storage lesions. PLoS One 2011 Mar 31;6(3):e18328.
        doi: 10.1371/journal.pone.0018328pubmed: 21483835google scholar: lookup
      8. Miglio A, Rocconi F, Cremonini V, D'Alessandro A, Reisz JA, Maslanka M, Lacroix IS, Tiscar G, Di Tommaso M, Antognoni MT. Effect of leukoreduction on the metabolism of equine packed red blood cells during refrigerated storage. J Vet Intern Med 2024 Mar-Apr;38(2):1185-1195.
        doi: 10.1111/jvim.17015pubmed: 38406982google scholar: lookup
      9. Spada E, Perego R, Baggiani L, Proverbio D. Haematological and morphological evaluation of feline whole blood units collected for transfusion purposes. J Feline Med Surg 2019 Aug;21(8):732-740.
        doi: 10.1177/1098612X18798841pubmed: 30226393google scholar: lookup
      10. Spada E, Proverbio D, Baggiani L, Bagnagatti De Giorgi G, Ferro E, Perego R. Change in haematological and selected biochemical parameters measured in feline blood donors and feline whole blood donated units. J Feline Med Surg 2017 Apr;19(4):375-381.
        doi: 10.1177/1098612X16628919pubmed: 26842942google scholar: lookup
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