Stored Stallion Sperm Quality Depends on Sperm Preparation Method in INRA82 or INRA96.
Abstract: Removal of seminal plasma facilitates stallion sperm survival during storage, but washing may damage sperm chromatin. Therefore, sperm quality was compared in samples following single-layer centrifugation (SLC) or sperm washing and controls (extension only) in two extenders, INRA82 and INRA96. Ejaculates from six stallions were split among six treatments: SLC, sperm washing, and controls, in INRA82 and INRA96. Sperm motility and acrosome status were evaluated at 0, 24, 48, 72 and 96 hours; morphology at 0, 24, 48, 72 hours and chromatin integrity at 0 and 96 hours, with storage at 6°C. Sperm samples in INRA96 had better motility, acrosome status, and normal morphology than samples in INRA82. The SLC samples had higher motility and fewer reacted acrosomes than controls, and lower fragmented chromatin than washed samples. Fewer spermatozoa with tail defects were observed after SLC than after sperm washing; spermatozoa washed in INRA82 had fewer tail defects than those washed in INRA96. In conclusion, sperm quality (except for morphology) was better in INRA96 than in INRA82 and was better in SLC samples than in washed samples or controls. The SLC method is a useful adjunct to stallion sperm preparation, especially for storage before artificial insemination.
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Publication Date: 2020-12-30 PubMed ID: 33663717DOI: 10.1016/j.jevs.2020.103367Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article explores how the quality of stored stallion sperm depends on different sperm preparation methods, specifically single-layer centrifugation (SLC) and sperm washing, in two extenders, INRA82 and INRA96.
Objective and Methodology
- The study’s main aim was to investigate the effects of different sperm preparation methods and extenders on the quality of stored stallion sperm. The researchers compared the results from single-layer centrifugation (SLC) and sperm washing against controls (extension only) in two extenders, INRA82 and INRA96.
- Six stallions provided the ejaculates, which were then split among six treatments. The methods of treatment included SLC, sperm washing, and controls, prepared in both INRA82 and INRA96 extenders.
Measurements and Evaluations
- Several aspects of sperm quality were evaluated at multiple time intervals (0, 24, 48, 72, and 96 hours) while stored at 6°C. The measured traits included sperm motility, acrosome status, morphology, and chromatin integrity.
- Chromatin integrity was notably examined only at initial (0 hour) and final (96 hour) stages.
Findings and Conclusion
- The results indicated that sperm samples in the INRA96 extender demonstrated better motility, more intact acrosomes, and more regular morphology when compared to those in INRA82.
- SLC samples showed higher motility and fewer reacted acrosomes compared to controls. They also exhibited lesser chromatin fragmentation than washed samples, which points towards better sperm viability.
- Additionally, it was observed that fewer sperm had tail defects after SLC than after sperm washing, with those washed in INRA82 exhibiting fewer tail defects than those washed in INRA96.
- The overall conclusion from the study suggests that, except for morphology, sperm quality was generally better in samples prepared in INRA96 than in INRA82, and better in SLC samples than in washed samples or controls.
- The study highlights the efficacy of the SLC method as a useful adjunct for stallion sperm preparation, specifically when storage is necessary prior to artificial insemination processes.
Cite This Article
APA
Papin J, Stuhtmann G, Martinsson G, Sieme H, Lundeheim N, Ntallaris T, Morrell JM.
(2020).
Stored Stallion Sperm Quality Depends on Sperm Preparation Method in INRA82 or INRA96.
J Equine Vet Sci, 98, 103367.
https://doi.org/10.1016/j.jevs.2020.103367 Publication
Researcher Affiliations
- Clinic for Horses - Unit for Reproductive Medicine, University of Veterinary Medicine Hannover, Hannover, Germany.
- National Stud Lower Saxony, Celle, Germany.
- National Stud Lower Saxony, Celle, Germany.
- Clinic for Horses - Unit for Reproductive Medicine, University of Veterinary Medicine Hannover, Hannover, Germany.
- Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden.
- Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
- Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden. Electronic address: jane.morrell@slu.se.
MeSH Terms
- Animals
- Horses
- Insemination, Artificial / veterinary
- Male
- Semen
- Semen Preservation / veterinary
- Sperm Motility
- Spermatozoa
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