Structure of dermatan sulfate. VII. The copolymeric structure of dermatan sulfate from horse aorta.
Abstract: The structure of dermatan sulfate-chondroitin sulfate
copolymers, isolated from horse aorta, has been examined.
It was found that a large proportion of the galactosaminoglycans of this tissue was obtained as a discrete polysaccharide fraction with an L-iduronic acid to D-glucuronic
acid ratio of approximately 1: 2. This finding together with
infrared data indicated that the polymer contained approximately equimolar proportions of the three repeating disaccharide units glucuronosyl-N-acetylgalactosamine 4-sulfate
(A), iduronosyl-N-acetylgalactosamine 4-sulfate (B), and
glucuronosyl-N-acetylgalactosamine 6-sulfate (C).
Degradation of the polymer with chondroitinase-AC
yielded, in addition to a large quantity of unsaturated disaccharides, a tetrasaccharide fraction corresponding to an
AB sequence in the original polymer. Degradation with
testicular hyaluronidase produced a tetrasaccharide of the
sequence CA, in addition to higher oligosaccharides corresponding to sequences like ABA or ABC.
By sequential degradation of the dermatan sulfatechondroitm sulfate copolymer with testicular hyaluronidase,
/3-glucuronidase, and chondroitinase-AC, it was possible to
obtain larger fragments containing the bulk of the B units
of the polymer. A detailed examination of these fragments
revealed that the majority of the B units were located in
clusters, particularly in the immediate vicinity of the carbohydrate-protein link. Furthermore, A units were prevalent in positions adjacent to B units, whereas the C units
occurred preferentially in the center of the glucuronic acidcontaining sections of the chain.
Publication Date: 1970-09-25 PubMed ID: 5456149
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- Journal Article
Summary
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The research article presents an analysis of the structural composition of dermatan sulfate-chondroitin sulfate copolymers found in horse aorta. It finds that the polymer comprises repeating units of certain disaccharides, with specific units being more prevalent in particular areas of the chain.
Examination of Polymer Structure
- The study involves an examination of the structure of dermatan sulfate-chondroitin sulfate copolymers, which are isolated from horse aorta.
- Through their research, the authors found that a significant proportion of the galactosaminoglycans (a type of polysaccharide) in the aorta was obtained as a distinct polysaccharide fraction. This fraction showed an approximate L-iduronic acid to D-glucuronic acid ratio of 1:2.
- This information, together with data gathered from infrared studies, led to the conclusion that the polymer contains almost equal proportions of three repeating disaccharide units, namely, glucuronosyl-N-acetylgalactosamine 4-sulfate (A), iduronosyl-N-acetylgalactosamine 4-sulfate (B), and glucuronosyl-N-acetylgalactosamine 6-sulfate (C).
Analyzing the Degradation of Polymer
- The degradation of the polymer was then carried out with chondroitinase-AC, which resulted in a large quantity of unsaturated disaccharides and a tetrasaccharide fraction aligning with an AB sequence in the original polymer.
- Further degradation with testicular hyaluronidase yielded a tetrasaccharide sequence CA, in addition to higher oligosaccharides corresponding to sequences like ABA or ABC.
Detailed Examination of the Polymer Fragments
- By conducting sequential degradation of the copolymer using testicular hyaluronidase, β-glucuronidase, and chondroitinase-AC, it was possible for the researchers to obtain larger fragments containing the majority of the B units of the polymer.
- Upon detailed examination of these fragments, it was discovered that most of the B units were clustered, particularly near the carbohydrate-protein connection.
- Additionally, A units were generally found in positions adjacent to B units, whereas C units preferentially occurred in the center of the sections of the chain containing glucuronic acid.
Cite This Article
APA
Fransson LA, Havsmark B.
(1970).
Structure of dermatan sulfate. VII. The copolymeric structure of dermatan sulfate from horse aorta.
J Biol Chem, 245(18), 4770-4783.
Publication
Researcher Affiliations
MeSH Terms
- Animals
- Aorta, Thoracic
- Chondroitin / analysis
- Chromatography, Gel
- Chromatography, Paper
- Disaccharides / analysis
- Electrophoresis
- Glucuronates / analysis
- Glucuronidase
- Hexosamines / analysis
- Horses
- Hyaluronoglucosaminidase
- Infrared Rays
- Lyases
- Male
- Oligosaccharides / analysis
- Polysaccharides / analysis
- Spectrum Analysis
- Sulfuric Acids
- Testis / enzymology
- Ultraviolet Rays
- Uronic Acids / analysis
Citations
This article has been cited 16 times.- Higashi K, Takeuchi Y, Mukuno A, Tomitori H, Miya M, Linhardt RJ, Toida T. Composition of glycosaminoglycans in elasmobranchs including several deep-sea sharks: identification of chondroitin/dermatan sulfate from the dried fins of Isurus oxyrinchus and Prionace glauca. PLoS One 2015;10(3):e0120860.
- Yamaguchi K, Tamaki H, Fukui S. Detection of oligosaccharide ligands for hepatocyte growth factor/scatter factor (HGF/SF), keratinocyte growth factor (KGF/FGF-7), RANTES and heparin cofactor II by neoglycolipid microarrays of glycosaminoglycan-derived oligosaccharide fragments. Glycoconj J 2006 Nov;23(7-8):513-23.
- Praillet C, Lortat-Jacob H, Grimaud JA. Interferon gamma differentially affects the synthesis of chondroitin/dermatan sulphate and heparan sulphate by human skin fibroblasts. Biochem J 1996 Sep 15;318 ( Pt 3)(Pt 3):863-70.
- Faissner A, Clement A, Lochter A, Streit A, Mandl C, Schachner M. Isolation of a neural chondroitin sulfate proteoglycan with neurite outgrowth promoting properties. J Cell Biol 1994 Aug;126(3):783-99.
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- Pearson CH, Gibson GJ. Proteoglycans of bovine periodontal ligament and skin. Occurrence of different hybrid-sulphated galactosaminoglycans in distinct proteoglycans. Biochem J 1982 Jan 1;201(1):27-37.
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- Webber RJ, Norby DP, Malemud CJ, Goldberg VM, Moskowitz RW. Characterization of newly synthesized proteoglycans from rabbit menisci in organ culture. Biochem J 1984 Aug 1;221(3):875-84.
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- Fransson LA, Cöster L, Malmstrom A, Sjöberg I. The copolymeric structure of pig skin dermatan suplhate. Characterization of D-glucuronic acid-containing oligosaccharides isolated after controlled degradation of oxydermatan sulphate. Biochem J 1974 Nov;143(2):369-78.
- Kimata K, Okayama M, Ooira A, Suzuki S. Cytodifferentiation and proteoglycan biosynthesis. Mol Cell Biochem 1973 Jun 27;1(2):211-28.
- Iverius PH. Coupling of glycosaminoglycans to agarose beads (sepharose 4B). Biochem J 1971 Oct;124(4):677-83.
- Rauch U, Glössl J, Kresse H. Comparison of small proteoglycans from skin fibroblasts and vascular smooth-muscle cells. Biochem J 1986 Sep 1;238(2):465-74.
- Chan L, Wong YC. Cytochemical localisation and characterisation of proteoglycans (glycosaminoglycans) in the epithelial-stromal interface of the seminal vesicle of the guinea pig. J Anat 1992 Feb;180 ( Pt 1)(Pt 1):41-56.
- Malström A, Carlstedt I, Aberg L, Fransson LA. The copolymeric structure of dermatan sulphate produced by cultured human fibroblasts. Different distribution of iduronic acid and glucuronic acid-containing units in soluble and cell-associated glycans. Biochem J 1975 Dec;151(3):477-89.
- Stevens RL, Colombo M, Gonzales JJ, Hollander W, Schmid K. The glycosaminoglycans of the human artery and their changes in atherosclerosis. J Clin Invest 1976 Aug;58(2):470-81.
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