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Immunology1975; 28(1); 187-197;

Studies on equine immunoglobulins. IV. Immunoglobulins of the donkey.

Abstract: Donkey IgGa was isolated in purified form from normal and immune donkey sera by column chromatography on DEAE-cellulose. Isolated donkey IgGa and mixtures of (IgGa+IgGb) were used as antigens to prepare rabbit reagents specific for equine IgGa or IgGb. Antibodies present in sera obtained from a single donkey at various times during the course of hyperimmunization with BSA were isolated by immuno-adsorption. The class or subclass of immunoglobulins present among isolated, donkey anti-BSA antibodies was determined by use of specific rabbit anti-equine immunoglobulin reagents. The homologues of horse IgGa, IgGb, IgGc and IgA were identified in normal donkey, mule, hinny and zebra serum.
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Publication Date: 1975-01-01 PubMed ID: 1116869PubMed Central: PMC1445747
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  • Journal Article
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study explores the presence and characteristics of various subclasses of immunoglobulins (antibodies) – specifically IgGa, IgGb, and IgGc – in ordinary and immune donkey serum. The researchers also investigated these immunoglobulins’ presence in the serum of other equine species such as mules, hinnies, and zebras.

Methodology and Objectives

  • The researchers isolated IgGa from both normal and immune donkey serum through a column chromatography process on DEAE-cellulose, a kind of ion-exchange resin.
  • Using isolated IgGa, and mixtures of IgGa and IgGb, they prepared rabbit reagents. These rabbit reagents are specific to the two immunoglobulin subclasses.
  • They also obtained serum from a single donkey at different points during BSA hyperimmunization. Then, they isolated the respective antibodies via immuno-adsorption and determined the class or subclass of immunoglobulins among isolated donkey anti-BSA antibodies.
  • The researchers’ primary goal was to identify the presence of the homologues of horse IgGa, IgGb, IgGc, and IgA in normal donkey serum and in the serum of other equine species, such as mules, hinnies, and zebras.

Findings and Importance

  • The study makes a significant contribution to understanding the immune system of donkeys and other equine species. It demonstrates the presence of specific antibody subclasses (IgGa, IgGb, IgGc, and IgA) in their serum.
  • Insights obtained from these investigations can inform the development of better, species-specific therapies in veterinary medicine, particularly for diseases that affect equine species.
  • The study also enhances the knowledge about the immune responses generated in these species in response to hyperimmunization, thereby contributing to immunological research focusing on equine species.

Cite This Article

APA
Allen PZ, Dalton EJ. (1975). Studies on equine immunoglobulins. IV. Immunoglobulins of the donkey. Immunology, 28(1), 187-197.

Publication

Immunology
ISSN: 0019-2805
NlmUniqueID: 0374672
Country: England
Language: English
Volume: 28
Issue: 1
Pages: 187-197

Researcher Affiliations

Allen, P Z
    Dalton, E J

      MeSH Terms

      • Animals
      • Antigens
      • Chromatography, DEAE-Cellulose
      • Chromatography, Ion Exchange
      • Horses / immunology
      • Immune Sera
      • Immunoelectrophoresis
      • Immunoglobulin A
      • Immunoglobulin Fab Fragments
      • Immunoglobulin Fc Fragments
      • Immunoglobulin G / isolation & purification
      • Immunoglobulins / isolation & purification
      • Perissodactyla / immunology
      • Rabbits / immunology
      • Serum Albumin, Bovine

      References

      This article includes 22 references
      1. PORTER RR. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain.. Biochem J 1959 Sep;73(1):119-26.
        pubmed: 14434282doi: 10.1042/bj0730119google scholar: lookup
      2. Klinman NR, Rockey JH, Frauenberger G, Karush F. Equine anti-hapten antibody. 3. The comparative properties of gamma G- and gammaA-antibodies.. J Immunol 1966 Apr;96(4):587-95.
        pubmed: 5936286
      3. ROCKEY JH, KLINMAN NR, KARUSH F. EQUINE ANTIHAPTEN ANTIBODY. I. 7S BETA-2A- AND 1OS GAMMA-1- GLOBULIN COMPONENTS OF PURIFIED ANTI-BETA-LACTOSIDE ANTIBODY.. J Exp Med 1964 Oct 1;120(4):589-609.
        pubmed: 14212122doi: 10.1084/jem.120.4.589google scholar: lookup
      4. KLIMAN NR, ROCKEY JH, KARUSH F. VALENCE AND AFFINITY OF EQUINE NONPRECIPITATING ANTIBODY TO A HAPTENIC GROUP.. Science 1964 Oct 16;146(3642):401-3.
        pubmed: 14186463doi: 10.1126/science.146.3642.401google scholar: lookup
      5. FLEISCHMAN JB, PAIN RH, PORTER RR. Reduction of gamma-globulins.. Arch Biochem Biophys 1962 Sep;Suppl 1:174-80.
        pubmed: 13945475
      6. RELYVELD EH, RAYNAUD M. [Influence of electrophoretic nature (gamma or beta globulin) of horse diphtheria antitoxin on form of quantitative precipitation curve].. Ann Inst Pasteur (Paris) 1957 Aug;93(2):246-50.
        pubmed: 13470441
      7. Allen PZ, Sirisinha S, Vaughan JH. Immunochemical studies on equine antibodies to human gamma-2-globulin.. J Immunol 1965 Nov;95(5):918-28.
        pubmed: 4158645
      8. Audibert F, Sandor G. [Study of the major antitoxin of equine serums (IgT), in relation to other immunoglobulins present in these serums].. Ann Inst Pasteur (Paris) 1972 Feb;122(2):221-49.
        pubmed: 4114707
      9. Allen PZ, Johnson JS. Studies on equine immunoglobulins. 3. Antigenic interrelationships among horse and dog G globulins.. Comp Biochem Physiol B 1972 Feb 15;41(2):371-83.
        pubmed: 4111331doi: 10.1016/0305-0491(72)90041-7google scholar: lookup
      10. Johnston SL, Allen PZ. Equine antibodies to human gamma-G-globulin. II. Isolation and antigenic analysis of gamma-2- and gamma-1-antibody fractions from equine antisera to human gamma-G-globulin.. J Immunol 1968 May;100(5):942-54.
        pubmed: 4171945
      11. Avrameas S, Ternynck T. Biologically active water-insoluble protein polymers. I. Their use for isolation of antigens and antibodies.. J Biol Chem 1967 Apr 10;242(7):1651-9.
        pubmed: 4164594
      12. Martineau RS, Allen PZ. Cross reactions of a panosyl-azoprotein conjugate with antipneumococcal horse sera.. Immunochemistry 1973 Dec;10(12):829-38.
        pubmed: 4148929doi: 10.1016/0019-2791(73)90187-0google scholar: lookup
      13. Lavergne M, Raynaud M. [A study of aggregating immunoglobulins in horses ("AI" or IgB or IgI)].. Ann Immunol (Paris) 1973 Feb;124(1):45-74.
        pubmed: 4146715
      14. Zolla S, Goodman JW. An aggregating immunoglobulin in hyperimmune equine anti-pneumococcal sera.. J Immunol 1968 Apr;100(4):880-97.
        pubmed: 4384580
      15. Sein H, Moroz C, de Vries A. Isolation of immunoglobulin and fragment from donkey anti-Russell's viper serum.. Ann Inst Pasteur (Paris) 1972 Feb;122(2):213-9.
        pubmed: 4625720
      16. Sandor G, Audibert F. [2 physiological functions of humoral immunity and the 2 proteins which assume it in the horse serum].. C R Acad Hebd Seances Acad Sci D 1970 Mar 16;270(11):1538-40.
        pubmed: 4985996
      17. Rockey JH. Equine antihapten antibody. The subunits and fragments of anti-beta-lactoside antibody.. J Exp Med 1967 Feb 1;125(2):249-75.
        pubmed: 4959973doi: 10.1084/jem.125.2.249google scholar: lookup
      18. Audibert F, Sandor G. [Nature of the antitoxin fraction of horse immune serums].. C R Acad Hebd Seances Acad Sci D 1968 Jul 22;267(4):457-8.
        pubmed: 4971202
      19. Allen PZ, Berger BM, Helms CM. An examination of the cross reactivity of human and equine gammaG globulins and their fragments by immunodiffusion.. J Immunol 1968 Nov;101(5):1023-35.
        pubmed: 4972230
      20. Vaerman JP, Querinjean P, Heremans JF. Studies on the IgA system of the horse.. Immunology 1971 Sep;21(3):443-54.
        pubmed: 5568324
      21. Sonoda S, Schlamowitz M. Studies of 125I trace labeling of immunoglobulin G by chloramine-T.. Immunochemistry 1970 Nov;7(11):885-98.
        pubmed: 5531224doi: 10.1016/0019-2791(70)90051-0google scholar: lookup
      22. KLINMAN NR, ROCKEY JH, KARUSH F. EQUINE ANTIHAPTEN ANTIBODY II. THE GAMMA-G(7S-GAMMA) COMPONENTS AND THEIR SPECIFIC INTERACTION.. Immunochemistry 1965 Mar;47:51-60.
        pubmed: 14290269

      Citations

      This article has been cited 6 times.
      1. Turini L, Bonelli F, Nocera I, Meucci V, Conte G, Sgorbini M. Evaluation of Different Methods to Estimate the Transfer of Immunity in Donkey Foals Fed with Colostrum of Good IgG Quality: A Preliminary Study. Animals (Basel) 2021 Feb 15;11(2).
        doi: 10.3390/ani11020507pubmed: 33672086google scholar: lookup
      2. Turini L, Bonelli F, Nocera I, Battaglia F, Meucci V, Panzani D, Mele M, Sgorbini M. Evaluation of jennies' colostrum: IgG concentrations and absorption in the donkey foals. A preliminary study. Heliyon 2020 Aug;6(8):e04598.
        doi: 10.1016/j.heliyon.2020.e04598pubmed: 32775752google scholar: lookup
      3. Matthews AG, Imlah P, McPherson EA. A reagin-like antibody in horse serum: 1. Occurrence and some biological properties. Vet Res Commun 1983 Jan;6(1):13-23.
        doi: 10.1007/BF02214891pubmed: 6868343google scholar: lookup
      4. Allen PZ, Glode M, Schneerson R, Robbins JB. Identification of immunoglobulin heavy-chain isotypes of specific antibodies of horse 46 group B meningococcal antiserum. J Clin Microbiol 1982 Feb;15(2):324-9.
        doi: 10.1128/jcm.15.2.324-329.1982pubmed: 6802867google scholar: lookup
      5. Allen PZ, Heidelberger M, Rappaport IA, Ward GM. Homologous and cross-reactive precipitins in anti-pneumococcal sera raised in mules. Immunology 1978 Jul;35(1):105-13.
        pubmed: 28285
      6. Turini L, Francesca B, Matilde V, Francesca B, Valentina M, Micaela S. Immunity transfer in mule foals fed with good IgG quality colostrum. Heliyon 2024 Feb 15;10(3):e25560.
        doi: 10.1016/j.heliyon.2024.e25560pubmed: 38327457google scholar: lookup
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