Study of Variation of ACOX1 Gene Among Different Horse Breeds Maintained in Iran.
Abstract: The gene is vital for fatty acid metabolism and is linked to environmental stress and physical exertion adaptation. The p.Asp237Ser variant (rs782885985) in is associated with increased enzyme activity and reactive oxygen species (ROS) levels. This study examined the polymorphism across six horse breeds in Iran: Arabian, Thoroughbred, KWPN, Caspian, Kurdish, and Turkmen. The goal was to identify differences in genotype distribution, potentially serving as genetic markers under selection pressure related to breed-specific traits. In a sample of 324 horses, genomic DNA was analyzed using PCR-RFLP, revealing three genotypes (TT, TG, GG). The GG genotype was most common in Kurdish and Arabian horses (86% and 70%, respectively), while the TT genotype was prevalent in Turkmen (24%) and Thoroughbred horses (23%). The T allele's frequency in Thoroughbred and Turkmen horses suggests that ACOX1 may be under selection pressure for phenotypic traits. Differences in genotype distribution were confirmed among breeds, with no sex-based association. The study concludes that is a potential genetic marker for horse performance and adaptability, emphasizing the importance of genetic diversity in breeding programs.
Publication Date: 2024-12-10 PubMed ID: 39765470PubMed Central: PMC11672723DOI: 10.3390/ani14243566Google Scholar: Lookup
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Summary
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This research explored the variation of the ACOX1 gene across six horse breeds in Iran to understand how differences in this gene may contribute to breed-specific traits.
Understanding the Study
Here is a detailed explanation of this research:
- The study focused on the ACOX1 gene, which plays a critical role in fatty acid metabolism. This gene is thought to be associated with how well organisms adapt to environmental stress and physical exertion. A particular variant of this gene, p.Asp237Ser variant (rs782885985), is known for increased enzyme activity and reactive oxygen species (ROS) levels.
- The research aimed to examine the variation of this gene across six different horse breeds maintained in Iran: Arabian, Thoroughbred, KWPN, Caspian, Kurdish, and Turkmen.
- The researchers’ goal was to identify any differences in ACOX1 genotype distribution across these breeds. Such differences could potentially serve as genetic markers, highlighting the specific traits that could be under selective pressure within each breed.
Methodology
The study used the following methods:
- A sample of 324 horses from the specified breeds was used for the study.
- Genomic DNA from each horse was analyzed using a method known as PCR-RFLP. This technique allows for the detection and analysis of specific regions of DNA.
Findings
The study found that:
- Three genotypes were identified from the ACOX1 gene: TT, TG, GG.
- The GG genotype was the most common among Kurdish and Arabian horses, appearing in 86% and 70% of horses from these breeds, respectively.
- Conversely, the TT genotype was most prevalent in Turkmen (24%) and Thoroughbred horses (23%). This suggests that the ACOX1 gene could be under selective pressure for certain traits within these breeds.
- Differences in the distribution of genotypes were found among the horse breeds, but there was no connection seen between genotype and the sex of the horses.
Conclusion
- The study concluded that the ACOX1 gene might serve as a potential genetic marker for horse performance and adaptability.
- This research emphasized the importance of promoting and maintaining genetic diversity in breeding programs, as various genotypes of particular genes can be linked to specific traits that are desirable within different breeds.
Cite This Article
APA
Boozarjomehri Amnieh S, Hassanpour A, Moghaddam S, Sakhaee F, Ropka-Molik K.
(2024).
Study of Variation of ACOX1 Gene Among Different Horse Breeds Maintained in Iran.
Animals (Basel), 14(24), 3566.
https://doi.org/10.3390/ani14243566 Publication
Researcher Affiliations
- Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz 5159115705, Iran.
- Department of Clinical Science, Tabriz Medical Sciences, Islamic Azad University, Tabriz 5159115705, Iran.
- Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran 1417935840, Iran.
- Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran 1963737611, Iran.
- Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.
Conflict of Interest Statement
The authors declare no conflicts of interest.
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