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Supportive techniques to investigate in vitro culture and cryopreservation efficiencies of equine ovarian tissue: A review.
Abstract: During the reproductive lifespan of a female, only a limited quantity of oocytes are naturally ovulated; therefore, the mammalian ovary possesses a substantial population of preantral follicles available to be handled and explored in vitro. Hence, the manipulation of preantral follicles enclosed in ovarian tissue aims to recover a considerable population of oocytes of high-value animals for potential application in profitable assisted reproductive technologies (ARTs). For this purpose, the technique of preantral follicle in vitro culture (IVC) has been the most common research tool, achieving extraordinary results with offspring production in the mouse model. Although promising outcomes have been generated in livestock animals after IVC of preantral follicles, the quantity and quality of embryo production with those oocytes are still poor. In recent years, the mare has become an additional model for IVC studies due to remarkable similarities with women and livestock animals regarding in vivo and in vitro ovarian folliculogenesis. For a successful IVC system, several factors should be carefully considered to provide an optimum culture environment able to support the viability and growth of preantral follicles enclosed in ovarian tissue. The cryopreservation of the ovarian tissue is another important in vitro manipulation technique that has been used to preserve the reproductive potential in humans and, in the future, may be used in highly valuable domestic animals or endangered species. Several improvements in cryopreservation protocols are necessary to support the utilization of ovarian tissue of different species in follow-up ARTs (e.g., ovarian fragment transplantation). This review aims to provide an update on the most current advances regarding supportive in vitro techniques used in equids to evaluate and manipulate preantral follicles and ovarian tissue, as well as methodological approaches used during IVC and cryopreservation techniques.
Copyright © 2020 Elsevier Inc. All rights reserved.
Publication Date: 2020-07-01 PubMed ID: 32891985DOI: 10.1016/j.theriogenology.2020.06.043Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Review
- Animal Science
- Animal Studies
- Assisted Reproductive Techniques
- Biotechnology
- Comparative Study
- Cryopreservation
- Embryo
- Endangered Species
- Equine Diseases
- Equine Health
- Equine model
- Equine Science
- Genetics
- In Vitro Research
- Oocyte
- Ovarian Follicles
- Reproductive Technology
- Species Comparison
- Veterinary Medicine
- Veterinary Research
Summary
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This research article presents a review of the latest advancements in techniques used to analyze and manipulate equine ovarian tissue and preantral follicles in laboratory conditions, as well as during in vitro culture and cryopreservation procedures. The study seeks to optimize the use of ovarian tissue in assisted reproductive technologies and contribute to safeguarding the reproductive potential of high-value domestic animals and endangered species.
Research Background
- The research delves into the exploration of preantral follicles present in ovary tissues of mammals. Preantral follicles, which carry a substantial population of unovulated oocytes, despite their quantity, are considered vital in assisted reproductive technologies (ARTs).
- The study points out that in vitro culture (IVC) is the most widely researched technique for handling these follicles. The process has been successful particularly in mice, leading to offspring production.
- The research highlights that the mare has become an additional model for IVC studies due to its resemblance to women and livestock animals in terms of in vivo and in vitro ovarian folliculogenesis.
In Vitro Culture (IVC)
- In Vitro Culture (IVC) is a technique that involves the cultivation of preantral follicles enclosed in ovarian tissue in a controlled, artificial environment. This method has demonstrated promising results in livestock animals.
- However, the study mentions that the embryo produced from the oocytes from IVC are of low quality and quantity.
- For the efficiency of the IVC system, various factors need to be controlled to provide an ideal culture environment that can sustain the viability and growth of the preantral follicles present in the ovarian tissue.
Cryopreservation
- Cryopreservation is another essential technique discussed in the research. This technique involves low-temperature preservation of ovarian tissue, aiming to preserve its reproductive potential.
- This approach has been beneficial in preserving human ovarian tissue and shows potential for use in valuable domestic animals and endangered species in the future.
- The study emphasizes that several improvements are needed in cryopreservation protocols to support the utilization of ovarian tissue of different species in future assisted reproductive technologies (ARTs) like ovarian fragment transplantation.
Study Objective
- The paper aims to shed light on the current advancements regarding the supportive in vitro techniques used in handling preantral follicles and ovarian tissue in mares. Specifically, the methodologies used during In Vitro Culture (IVC) and the cryopreservation techniques are reviewed.
Cite This Article
APA
Aguiar FLN, Gastal GDA, Alves KA, Alves BG, Figueiredo JR, Gastal EL.
(2020).
Supportive techniques to investigate in vitro culture and cryopreservation efficiencies of equine ovarian tissue: A review.
Theriogenology, 156, 296-309.
https://doi.org/10.1016/j.theriogenology.2020.06.043 Publication
Researcher Affiliations
- Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Ceará, Brazil; Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraíba, Sousa, Paraíba, Brazil. Electronic address: francisco.aguiar@ifpb.edu.br.
- Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA; National Institute of Agricultural Research, INIA La Estanzuela, Colonia, Uruguay.
- Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Ceará, Brazil; Postgraduate Program of Gynecology and Obstetrics, Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
- Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Ceará, Brazil; Animal Bioscience Postgraduate Program, Federal University of Goiás, Jataí, Goiás, Brazil.
- Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Ceará, Brazil.
- Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA. Electronic address: egastal@siu.edu.
MeSH Terms
- Animals
- Cryopreservation / veterinary
- Female
- Horses
- Mammals
- Oocytes
- Ovarian Follicle
- Ovary
- Tissue Culture Techniques / veterinary
Citations
This article has been cited 5 times.- Hyde KA, Aguiar FLN, Alvarenga PB, Rezende AL, Alves BG, Alves KA, Gastal GDA, Gastal MO, Gastal EL. Characterization of preantral follicle clustering and neighborhood patterns in the equine ovary. PLoS One 2022;17(10):e0275396.
- Azevedo AR, Pais AS, Almeida-Santos T, Pires VMR, Pessa P, Marques CC, Nolasco S, Castelo-Branco P, Prates JAM, Lopes-da-Costa L, Laranjo M, Botelho MF, Pereira RMLN, Pimenta JMBGA. Medical Grade Honey as a Promising Treatment to Improve Ovarian Tissue Transplantation. Bioengineering (Basel) 2022 Jul 30;9(8).
- Mohammadzadeh F, Ajdary M, Mohammadzadeh A, Safdarian L, Amidi F, Sobhani A. Leukemia inhibitory factor's effect on the growth and survival of sheep's follicles of ovarian tissue during vitrification. Cell Tissue Bank 2023 Mar;24(1):109-123.
- Souza SS, Aguiar FLN, Alves BG, Alves KA, Brandão FAS, Brito DCC, Raposo RDS, Gastal MO, Rodrigues APR, Figueiredo JR, Teixeira DÍA, Gastal EL. Equine ovarian tissue xenografting: impacts of cooling, vitrification, and VEGF. Reprod Fertil 2021 Dec;2(4):251-266.
- Maeda N. Brief Overview of Ice Nucleation. Molecules 2021 Jan 13;26(2).
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