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Veterinary medicine and science2026; 12(2); e70854; doi: 10.1002/vms3.70854

Survey on Faecal Microbiota Transplantation and Probiotic Use in Equine Practice in France and Belgium.

Abstract: Faecal microbiota transplantation (FMT) and probiotics are used in equine practice. Understanding veterinarians' perceptions and practices is crucial for effective implementation. Objective: (1) Evaluate the prevalence, usage patterns and perceived effectiveness of probiotics and FMT among equine veterinarians in France and Belgium. (2) Assess their knowledge, practices and influencing factors across demographics and settings. (3) Explore links between FMT protocols and treatment satisfaction. Methods: Cross-sectional survey. Methods: An online survey collected demographic data and responses on the use of probiotics and FMT. Analyses included descriptive statistics, chi-square tests and logistic regression models. Results: Ninety-six equine veterinarians participated, practicing in Belgium (52.1%), France (39.6%) or both (8.3%). Probiotic use was reported by 82.1%, more frequent in field than clinical practice (odds ratio [OR] = 3.61, 95% CI [1.09, 12.02], p = 0.036) and in France than Belgium (OR = 5.08, 95% CI [1.44, 17.94], p = 0.012). Probiotics were used for chronic diarrhoea (88.0%), acute diarrhoea (67.6%) and inflammatory bowel diseases (45.9%). Most veterinarians (83.3%) defined probiotics well, but 16.7% misidentified non-probiotic products. FMT was used by 76.0%, mainly occasionally and therapeutically, more in clinical than field practice (OR = 4.79, 95% CI [1.03, 22.27], p = 0.046). In theory, 58.3% prioritized infection-free donors, but only 22.5% tested donors before FMT, mostly using coprology (93.8%). Those who tested donors reported higher perceived efficacy (p = 0.0029). Conclusions: Potential selection bias, as participation was voluntary. Generalizability might be limited by focus on France and Belgium. Sample size, while informative, should be expanded. Conclusions: Probiotics and FMT were commonly used therapeutically by equine veterinarians in France and Belgium. Although probiotic use was widespread, some misunderstandings remained. FMT protocols varied, with donor faeces often untested. Treatment satisfaction was generally positive but estimated success rates varied. Standardized FMT protocols are needed to improve outcomes and consistency.
Publication Date: 2026-03-05 PubMed ID: 41782495PubMed Central: PMC12961356DOI: 10.1002/vms3.70854Google Scholar: Lookup
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  • Journal Article

Cite This Article

APA
Loublier C, Taminiau B, Seidel L, Moula N, Tano C, Cesarini C, Costa M, Lecoq L, Daube G, Amory H. (2026). Survey on Faecal Microbiota Transplantation and Probiotic Use in Equine Practice in France and Belgium. Vet Med Sci, 12(2), e70854. https://doi.org/10.1002/vms3.70854

Publication

ISSN: 2053-1095
NlmUniqueID: 101678837
Country: England
Language: English
Volume: 12
Issue: 2
Pages: e70854
PII: e70854

Researcher Affiliations

Loublier, Clémence
  • Equine Clinical Department, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
  • Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
Taminiau, Bernard
  • Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
  • Department of Food Sciences-Microbiology, Faculty of Veterinary Medicine, University of Liege, Liège, Belgium.
Seidel, Laurence
  • Biostatistics and Research Method Center (B-STAT), CHU-ULiège, Liège, Belgium.
Moula, Nassim
  • GIGA Animal Facilities, University of Liège, Liège, Belgium.
Tano, Colombe
  • Equine Clinical Department, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
Cesarini, Carla
  • Equine Clinical Department, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
  • Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
Costa, Marcio
  • Department of Veterinary Biomedicine, University of Montreal, Saint-Hyacinthe, Quebec, Canada.
Lecoq, Laureline
  • Equine Clinical Department, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
  • Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
Daube, Georges
  • Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
  • Department of Food Sciences-Microbiology, Faculty of Veterinary Medicine, University of Liege, Liège, Belgium.
Amory, Hélène
  • Equine Clinical Department, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
  • Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.

MeSH Terms

  • Belgium
  • Probiotics / therapeutic use
  • Horses
  • France
  • Animals
  • Fecal Microbiota Transplantation / veterinary
  • Fecal Microbiota Transplantation / statistics & numerical data
  • Cross-Sectional Studies
  • Horse Diseases / therapy
  • Veterinarians / statistics & numerical data
  • Veterinarians / psychology
  • Female
  • Male
  • Surveys and Questionnaires
  • Humans

Grant Funding

  • 40005849 / Fonds De La Recherche Scientifique - FNRS

Conflict of Interest Statement

The authors declare no conflicts of interest.

References

This article includes 53 references
  1. Arantes, J. A. , Di Pietro R., Ratté M., Arroyo L. G., Leclère M., and Costa M. C.. 2025. “Changes in Bacterial Viability After Preparation and Storage of Fecal Microbiota Transplantation Solution Using Equine Feces.” Peer Journal 13: e18860.
    pmc: PMC11847485pubmed: 39989751
  2. Baunwall, S. M. D. , Terveer E. M., Dahlerup J. F., et al. 2021. “The Use of Faecal Microbiota Transplantation (FMT) in Europe: A Europe‐Wide Survey.” Lancet Regional Health—Europe 9: 100181.
    pmc: PMC8513118pubmed: 34693388
  3. Bell, J. , Raidal S. L., Cuming R. S., Trope G., and Hughes K. J.. 2024. “Effects of Fecal Microbiota Transplantation on Clinical Outcomes and Fecal Microbiota of Foals With Diarrhea.” Veterinary Internal Medicine 38, no. 5: 2718–2728.
    pmc: PMC11423448pubmed: 39266472
  4. Benech, N. , Legendre P., Radoszycki L., Varriale P., and Sokol H.. 2022. “Patient Knowledge of Gut Microbiota and Acceptability of Fecal Microbiota Transplantation in Various Diseases.” Neurogastroenterology & Motility 34: e14320.
    pubmed: 35037353
  5. Berreta, A. , Burbick C. R., Alexander T., Kogan C., and Kopper J. J.. 2021. “Microbial Variability of Commercial Equine Probiotics.” Journal of Equine Veterinary Science 106: 103728.
    pubmed: 34670695
  6. Boscan, P. , Van Hoogmoed L. M., Farver T. B., and Snyder J. R.. 2006. “Evaluation of the Effects of the Opioid Agonist Morphine on Gastrointestinal Tract Function in Horses.” American Journal of Animal and Veterinary Sciences 67, no. 6: 992–997.
    pubmed: 16740092
  7. Boucher, L. , Leduc L., Leclère M., and Costa M. C.. 2024. “Current Understanding of Equine Gut Dysbiosis and Microbiota Manipulation Techniques: Comparison With Current Knowledge in Other Species.” Animals 14, no. 5: 758.
    pmc: PMC10931082pubmed: 38473143
  8. Boyle, A. G. 2021. “Respiratory Distress in the Adult and Foal.” Veterinary Clinics of North America: Equine Practice 37, no. 2: 311–325.
    pubmed: 34119402
  9. Brandt, L. J. , and Aroniadis O. C.. 2013. “An Overview of Fecal Microbiota Transplantation: Techniques, Indications, and Outcomes.” Gastrointestinal Endoscopy 78, no. 2: 240–249.
    pubmed: 23642791
  10. Cammarota, G. , Ianiro G., Kelly C. R., et al. 2019. “International Consensus Conference on Stool Banking for Faecal Microbiota Transplantation in Clinical Practice.” Gut 68, no. 12: 2111–2121.
    pmc: PMC6872442pubmed: 31563878
  11. Costa, M. C. , Arroyo L. G., Allen‐Vercoe E., et al. 2012. “Comparison of the Fecal Microbiota of Healthy Horses and Horses With Colitis by High Throughput Sequencing of the V3–V5 Region of the 16S rRNA Gene.” PLoS ONE 7, no. 7: e41484.
    pmc: PMC3409227pubmed: 22859989
  12. Costa, M. C. , Stämpfli H. R., Arroyo L. G., Allen‐Vercoe E., Gomes R. G., and Weese J.. 2015. “Changes in the Equine Fecal Microbiota Associated With the Use of Systemic Antimicrobial Drugs.” BMC Veterinary Research 11, no. 1: 19.
    pmc: PMC4323147pubmed: 25644524
  13. Costa, M. , Di Pietro R., Bessegatto J. A., et al. 2021. “Evaluation of Changes in Microbiota After Fecal Microbiota Transplantation in 6 Diarrheic Horses.” Canadian Veterinary Journal 62, no. 10: 1123–1130.
    pmc: PMC8439339pubmed: 34602643
  14. nnDesrochers, A. M.n, Dolente B. A., Roy M. F., Boston R., and Carlisle S.. 2005. “Efficacy of for Treatment of Horses With Acute Enterocolitis.” Journal of the American Veterinary Medical Associationn227, no. 6: 954–959.n
    pubmed: 16190596
  15. Destrez, A. , Grimm P., Cézilly F., and Julliand V.. 2015. “Changes of the Hindgut Microbiota Due to High‐Starch Diet Can be Associated With Behavioral Stress Response in Horses.” Physiology & Behavior 149: 159–164.
    pubmed: 26048306
  16. Dougal, K. , de la Fuente G., Harris P. A., et al. 2014. “Characterisation of the Faecal Bacterial Community in Adult and Elderly Horses Fed a High Fibre, High Oil or High Starch Diet Using 454 Pyrosequencing.” PLoS ONE 9, no. 2: e87424.
    pmc: PMC3913607pubmed: 24504261
  17. Feary, D. J. , and Hassel D. M.. 2006. “Enteritis and Colitis in Horses.” Veterinary Clinics of North America: Equine Practice 22, no. 2: 437–479.
    pubmed: 16882483
  18. Fernandes, K. A. , Kittelmann S., Rogers C. W., et al. 2014. “Faecal Microbiota of Forage‐Fed Horses in New Zealand and the Population Dynamics of Microbial Communities Following Dietary Change. Forster RJ, editor.” PLoS ONE 9 no. 11: e112846.
    pmc: PMC4226576pubmed: 25383707
  19. Hill, C. , Guarner F., Reid G., et al. 2014. “The International Scientific Association for Probiotics and Prebiotics Consensus Statement on the Scope and Appropriate Use of the Term Probiotic.” Nature Reviews Gastroenterology & Hepatology 11, no. 8: 506–514.
    pubmed: 24912386
  20. nnIaniro, G.n, Maida M., Burisch J., et al. 2018. “Efficacy of Different Faecal Microbiota Transplantation Protocols for Infection: A Systematic Review and Meta‐Analysis.” UEG Journaln6, no. 8: 1232–1244.n
    pmc: PMC6169051pubmed: 30288286
  21. nnJohn, J.n, Roediger K., Schroedl W., Aldaher N., and Vervuert I.. 2015. “Development of Intestinal Microflora and Occurrence of Diarrhoea in Sucking Foals: Effects of Var. Toyoi Supplementation.” BMC Veterinary Researchn11, no. 1: 34.n
    pmc: PMC4333172pubmed: 25889817
  22. Kamada, N. , Seo S. U., Chen G. Y., and Núñez G.. 2013. “Role of the Gut Microbiota in Immunity and Inflammatory Disease.” Nature Reviews Immunology 13, no. 5: 321–335.
    pubmed: 23618829
  23. Kinoshita, Y. , Niwa H., Uchida‐Fujii E., Nukada T., and Ueno T.. 2022. “Simultaneous Daily Fecal Microbiota Transplantation Fails to Prevent Metronidazole‐Induced Dysbiosis of Equine Gut Microbiota.” Journal of Equine Veterinary Science 114: 104004.
    pubmed: 35526726
  24. Laustsen, L. , Edwards J. E., Hermes G. D. A., et al. 2021. “Free Faecal Water: Analysis of Horse Faecal Microbiota and the Impact of Faecal Microbial Transplantation on Symptom Severity.” Animals : An Open Access Journal From MDPI 11, no. 10: 2776.
    pmc: PMC8533009pubmed: 34679798
  25. Loublier, C. , Costa M., Taminiau B., et al. 2025. “Longitudinal Changes in Fecal Microbiota during Hospitalization in Horses With Different Types of Colic.” Veterinary Internal Medicine 39, no. 2: e70039.
    pmc: PMC11884602pubmed: 40048584
  26. Loublier, C. , Taminiau B., Heinen J., et al. 2023. “Evaluation of Bacterial Composition and Viability of Equine Feces After Processing for Transplantation.” Microorganisms 11, no. 2: 231.
    pmc: PMC9966902pubmed: 36838196
  27. Mach, N. , Ruet A., Clark A., et al. 2020. “Priming for Welfare: Gut Microbiota Is Associated With Equitation Conditions and Behavior in Horse Athletes.” Scientific Reports 10, no. 1: 8311.
    pmc: PMC7239938pubmed: 32433513
  28. McKinney, C. A. , Bedenice D., Pacheco A. P., et al. 2021. “Assessment of Clinical and Microbiota Responses to Fecal Microbial Transplantation in Adult Horses With Diarrhea.” PLoS ONE 16, no. 1: e0244381.
    pmc: PMC7808643pubmed: 33444319
  29. McKinney, C. A. , Oliveira B. C. M., Bedenice D., et al. 2020. “The Fecal Microbiota of Healthy Donor Horses and Geriatric Recipients Undergoing Fecal Microbial Transplantation for the Treatment of Diarrhea.” PLoS ONE 15, no. 3: e0230148.
    pmc: PMC7064224pubmed: 32155205
  30. Mullen, K. R. , Yasuda K., Divers T. J., and Weese J. S.. 2018. “Equine Faecal Microbiota Transplant: Current Knowledge, Proposed Guidelines and Future Directions.” Equine Veterinary Education 30, no. 3: 151–160.
    pmc: PMC7159401pubmed: 32313396
  31. nnParraga, M. E.n, Spier S. J., Thurmond M., and Hirsh D.. 1997. “A Clinical Trial of Probiotic Administration for Prevention of Shedding in the Postoperative Period in Horses With Colic.” Veterinary Internal Medicinen11, no. 1: 36–41.
    pubmed: 9132482
  32. Pietro, R. D. , Arroyo L. G., Leclere M., and Costa M.. 2023. “Effects of Concentrated Fecal Microbiota Transplant on the Equine Fecal Microbiota After Antibiotic‐Induced Dysbiosis.” Canadian Journal of Veterinary Research 87, no. 2: 85–96.
    pmc: PMC10069150pubmed: 37020579
  33. nnQuraishi, M. N.n, Widlak M., Bhala N., et al. 2017. “Systematic Review With Meta‐Analysis: The Efficacy of Faecal Microbiota Transplantation for the Treatment of Recurrent and Refractory Infection.” Alimentary Pharmacology & Therapeuticsn46, no. 5: 479–493.n
    pubmed: 28707337
  34. Ribeiro, G. , Ferri A., Clarke G., and Cryan J. F.. 2022. “Diet and the Microbiota–Gut–Brain‐Axis: A Primer for Clinical Nutrition.” Current Opinion in Clinical Nutrition & Metabolic Care 25, no. 6: 443–450.
    pmc: PMC9553262pubmed: 36102353
  35. Salem, S. E. , Maddox T. W., Antczak P., Ketley J. M., Williams N. J., and Archer D. C.. 2019. “Acute Changes in the Colonic Microbiota Are Associated With Large Intestinal Forms of Surgical Colic.” BMC Veterinary Research 15, no. 1: 468.
    pmc: PMC6925886pubmed: 31864369
  36. Schoster, A. , Staempfli H. R., Guardabassi L. G., Jalali M., and Weese J. S.. 2017. “Comparison of the Fecal Bacterial Microbiota of Healthy and Diarrheic Foals at Two and Four Weeks of Life.” BMC Veterinary Research 13, no. 1: 144.
    pmc: PMC5450145pubmed: 28558788
  37. Schoster, A. , Weese J. S., and Guardabassi L.. 2014. “Probiotic Use in Horses—What Is the Evidence for Their Clinical Efficacy?” Journal of Veterinary Internal Medicine 28, no. 6: 1640–1652.
    pmc: PMC4895607pubmed: 25231539
  38. Shaw, S. D. , and Stämpfli H.. 2018. “Diagnosis and Treatment of Undifferentiated and Infectious Acute Diarrhea in the Adult Horse.” Veterinary Clinics of North America: Equine Practice 34, no. 1: 39–53.
    pmc: PMC7134835pubmed: 29426709
  39. nnSlykerman, R. F.n, Hood F., Wickens K., et al. 2017. “Effect of HN001 in Pregnancy on Postpartum Symptoms of Depression and Anxiety: a Randomised Double‐Blind Placebo‐Controlled Trial.” EBioMedicinen24: 159–165.n
    pmc: PMC5652021pubmed: 28943228
  40. Steelman, S. M. , Chowdhary B. P., Dowd S., Suchodolski J., and Janečka J. E.. 2012. “Pyrosequencing of 16S rRNA Genes in Fecal Samples Reveals High Diversity of Hindgut Microflora in Horses and Potential Links to Chronic Laminitis.” BMC Veterinary Research 8, no. 1: 231.
    pmc: PMC3538718pubmed: 23186268
  41. Sun, S. , Chang G., and Zhang L.. 2022. “The Prevention Effect of Probiotics Against Eczema in Children: An Update Systematic Review and Meta‐Analysis.” Journal of Dermatological Treatment 33, no. 4: 1844–1854.
    pubmed: 34006167
  42. Tanabe, S. , Suzuki T., Wasano Y., et al. 2014. “Anti‐Inflammatory and Intestinal Barrier–Protective Activities of Commensal Lactobacilli and Bifidobacteria in Thoroughbreds: Role of Probiotics in Diarrhea Prevention in Neonatal Thoroughbreds.” Journal of Equine Science 25, no. 2: 37–43.
    pmc: PMC4090357pubmed: 25013357
  43. Theelen, M. J. P. , Luiken R. E. C., Wagenaar J. A., van Oldruitenborgh‐Oosterbaan M. M. S., Rossen J. W. A., and Zomer A. L.. 2021. “The Equine Faecal Microbiota of Healthy Horses and Ponies in The Netherlands: Impact of Host and Environmental Factors.” Animals 11, no. 6: 1762.
    pmc: PMC8231505pubmed: 34204691
  44. Tuniyazi, M. , Wang W., Fu Y., Hu X., and Zhang N.. 2023. “A Systematic Review of Current Applications of Fecal Microbiota Transplantation in Horses.” Animals 13, no. 8: 1304.
    pmc: PMC10141098pubmed: 37104445
  45. Van Den Abbeele, P. , Gérard P., Rabot S., et al. 2011. “Arabinoxylans and Inulin Differentially Modulate the Mucosal and Luminal Gut Microbiota and Mucin‐Degradation in Humanized Rats.” Environmental Microbiology 13, no. 10: 2667–2680.
    pubmed: 21883787
  46. Ward, M. P. , Alinovi C. A., Couëtil L. L., Glickman L. T., and Wu C. C.. 2004. “A Randomized Clinical Trial Using Probiotics to Prevent Salmonella Fecal Shedding in Hospitalized Horses.” Journal of Equine Veterinary Science 24, no. 6: 242–247.
  47. Weese, J. S. 2002. “Microbiologic Evaluation of Commercial Probiotics.” Journal of the American Veterinary Medical Association 220, no. 6: 794–797.
    pubmed: 11918274
  48. Weese, J. S. 2025. “Evaluation of Publication Bias in the Assessment of Probiotic Treatment for Gastrointestinal Disease in Dogs and Cats.” Canadian Veterinary Journal 66, no. 3: 250–254.
    pmc: PMC11897921pubmed: 40084240
  49. Weese, J. S. , Holcombe S. J., Embertson R. M., et al. 2015. “Changes in the Faecal Microbiota of Mares Precede the Development of Post Partum Colic.” Equine Veterinary Journal 47, no. 6: 641–649.
    pubmed: 25257320
  50. Weese, J. S. , and Rousseau J.. 2005. “Evaluation of Lactobacillus Pentosus WE7 for Prevention of Diarrhea in Neonatal Foals.” Journal of the American Veterinary Medical Association 226, no. 12: 2031–2034.
    pubmed: 15989186
  51. Williams, S. , Horner J., Orton E., et al. 2015. “Water Intake, Faecal Output and Intestinal Motility in Horses Moved From Pasture to a Stabled Management Regime With Controlled Exercise.” Equine Veterinary Journal 47, no. 1: 96–100.
    pmc: PMC4303976pubmed: 24528106
  52. Willing, B. , Vörös A., Roos S., Jones C., Jansson A., and Lindberg J. E.. 2009. “Changes in Faecal Bacteria Associated With Concentrate and Forage‐Only Diets Fed to Horses in Training.” Equine Veterinary Journal 41, no. 9: 908–914.
    pubmed: 20383990
  53. Yuyama, T. , Yusa S., Takai S., Tsubaki S., Kado Y., and Morotomi M.. 2004. “Evaluation of a Host‐Specific Lactobacillus Probiotic in Neonatal Foals.” The International Journal of Applied Research in Veterinary Medicine 2, no. 1: 26–33.

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