Survival of 59Fe-labeled erythrocytes in cross-transfused equine blood.
Abstract: Whole blood containing 59Fe-labeled erythrocytes (RBC) and unlabeled serum was transfused from a donor horse on 2 occasions into each of 6 recipient horses. Survival of transfused cells was monitored in the recipients as a function of time after transfusion by measuring RBC radioactivity in the recipients. After the 1st transfusion, RBC concentration of 59Fe remained at 60% to 100% of the transfused dose for 4 days, after which radioactivity values dropped to less than 10% of the dose by 6 days in 3 horses. In the 3 other horses, RBC radioactivity dropped immediately after transfusion, reaching minimal values in approximately 48 hours. After the 2nd transfusion, 1 horse retained 80% of the dose in circulating RBC for 4 days; 2 horses demonstrated a rapid loss of circulating radiolabeled RBC, reaching minimal values in 48 hours; and 2 horses demonstrated minimal radioactivity in the RBC mass even immediately after the transfusion. One horse died of anaphylactic shock during the 2nd transfusion. Erythrocyte compatibility tests, using the direct agglutination test, the antiglobulin test, and the hemolytic test, were not effective in predicting survival of transfused RBC.
Publication Date: 1978-04-01 PubMed ID: 646196
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- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research focused on examining how well transfused, iron-labeled red blood cells survive in horses. The study found that the survival rate of these cells, after being transferred into oth+er horses, varied significantly – some retaining a high percentage of the iron-labeled cells for days while others showing a rapid drop off.
Research Methodology
- The researchers transfused iron (59Fe)-labeled red blood cells, which were mixed with unlabeled serum, from one donor horse into six other horses.
- This process of transfusion was carried out twice and in both instances, the survivability of the transfused cells was monitored through time by measuring the level of radioactivity in the red blood cells.
Results and Observations
- In the first transfusion, the concentration of labelled red blood cells ranged between 60% to 100% of the transferred dose for four days. Following this, the radioactivity levels plunged to less than 10% in three horses by the sixth day.
- In the three remaining horses, the radioactivity rapidly decayed, hitting minimal levels within 48 hours.
- During the second transfusion, different horses demonstrated varying responses. For one horse, 80% of the dose remained in the circulating red blood cells for four days. However, two horses showed a rapid decline in the radiolabeled red blood cells, hitting minimal levels within 48 hours. Interestingly, two other horses showcased minimal radioactivity even immediately after the transfusion.
- One horse experienced an anaphylactic shock and died during the second transfusion.
- Throughout the study, traditional tests for erythrocyte compatibility such as direct agglutination, antiglobulin, and the hemolytic tests were not effective in predicting the survival of the transfused red blood cells.
Conclusion
- The study concluded that the survival of transfused 59Fe-labeled red blood cells in horses varied significantly. Therefore, traditional methods of predicting the survival of these cells were deemed ineffective.
Cite This Article
APA
Kallfelz FA, Whitlock RH, Schultz RD.
(1978).
Survival of 59Fe-labeled erythrocytes in cross-transfused equine blood.
Am J Vet Res, 39(4), 617-620.
Publication
Researcher Affiliations
MeSH Terms
- Animals
- Blood Group Incompatibility / blood
- Blood Group Incompatibility / veterinary
- Blood Transfusion / veterinary
- Erythrocyte Aging
- Horse Diseases / blood
- Horses / blood
Citations
This article has been cited 3 times.- Casenave P, Leclere M, Beauchamp G, Blais MC. Modified stall-side crossmatch for transfusions in horses. J Vet Intern Med 2019 Jul;33(4):1775-1783.
- Tomlinson JE, Taberner E, Boston RC, Owens SD, Nolen-Walston RD. Survival Time of Cross-Match Incompatible Red Blood Cells in Adult Horses. J Vet Intern Med 2015 Nov-Dec;29(6):1683-8.
- Cotter SM. Clinical transfusion medicine. Adv Vet Sci Comp Med 1991;36:187-223.
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