Survival of equine embryos co-cultured with equine oviductal epithelium from the four- to eight-cell to the blastocyst stage after transfer to synchronous recipient mares.
Abstract: In this study we examined the ability of equine oviductal epithelial cells (OEC) to support the development of four- to eight-cell equine embryos in vitro and investigated the ability of co-cultured embryos to continue normal development after transfer to synchronous recipient mares. Equine embryos obtained at Day 2 after ovulation were cultured with or without OEC for 5 days. Those OEC co-cultured embryos that reached the blastocyst stage and embryos recovered from the uterus at Day 7 were surgically transferred to synchronous recipient mares. Co-culture with OEC improved (P < 0.01) development of four- to eight-cell embryos to blastocysts compared to medium alone (11/15 vs 0/6) during 5 days in vitro. Embryos co-cultured with OEC were smaller (P < 0.05) and more delayed in development than Day-7 uterine blastocysts. There was no difference in the Day-30 survival rate of co-cultured blastocysts (3/8) or Day-7 uterine blastocysts (5/8) after transfer to recipient mares. These results indicate that co-culture with OEC can support development of four- to eight-cell equine embryos in vitro and that co-cultured embryos can continue normal development after transfer to recipient mares.
Publication Date: 1992-05-01 PubMed ID: 16727097DOI: 10.1016/0093-691x(92)90097-bGoogle Scholar: Lookup
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- Journal Article
Summary
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This research studied the effectiveness of using equine oviductal epithelial cells in aiding the development of four- to eight-cell equine embryos in vitro, as well as the success rate of these embryos following transfer to recipient mares.
Objective of the Study
- The main objective was to determine if equine oviductal epithelial cells (OEC) had the capacity to support the growth of four- to eight-cell equine embryos in vitro.
- The study also aimed to investigate the viability of the developed embryos post-transfer to recipient mares.
Methodology
- Equine embryos acquired on the second day after ovulation were cultured with or without OEC for five days.
- The embryos that developed to the blastocyst stage with the aid of OEC, as well as the embryos recovered from the uterus at Day 7, were surgically transferred to recipient mares.
Findings
- Co-culturing with OEC substantially enhanced the development of four- to eight-cell embryos into blastocysts compared to those cultured in a medium alone over five days in vitro.
- However, the embryos cultivated with the help of OEC were noted as being smaller and less advanced in their development compared to the Day-7 uterus blastocysts.
- The survival rate of co-cultured blastocysts at Day-30 was not significantly different from that of Day-7 uterine blastocysts after being transferred to recipient mares.
Conclusion
- The findings from the study suggest that co-culturing with OEC supports the in vitro development of four- to eight-cell equine embryos.
- Moreover, it was demonstrated that the developed co-cultured embryos can continue to develop normally once transferred to recipient mares.
Cite This Article
APA
Ball BA, Miller PG.
(1992).
Survival of equine embryos co-cultured with equine oviductal epithelium from the four- to eight-cell to the blastocyst stage after transfer to synchronous recipient mares.
Theriogenology, 37(5), 979-991.
https://doi.org/10.1016/0093-691x(92)90097-b Publication
Researcher Affiliations
- Department of Clinical Sciences Cornell University, Ithaca, New York 14853 USA.
Citations
This article has been cited 1 times.- Nematollahi N, Valojerdi MR. Effect of Vero cell coculture on the development of frozen-thawed two-cell mouse embryos. J Assist Reprod Genet 1999 Aug;16(7):380-4.
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