T and B lymphocytes in horses persistently infected with equine infectious anaemia virus.
Abstract: The percentage of T and B lymphocytes in the peripheral blood of horses chronically infected with equine infectious anaemia (EIA) virus was determined and the results were compared with the percentage of these cells in healthy uninfected horses. Cells with membrane receptors for sheep erythrocytes (T and active T lymphocytes) were determined by E and A rosette techniques, while cells with receptors for the C3b component of complement and those with receptors for mouse erythrocytes (B lymphocytes), were determined by the EAC rosette method. The percentage of Fe positive cells was assayed by the EA rosette test. The majority of peripheral blood lymphocytes (PBL) from both uninfected and EIA-infected horses formed rosettes of each kind with only three erythrocytes indicating a low density of the corresponding receptors on the cell membrane under the condition of the assays used. The percentage of T lymphocytes in the peripheral blood of diseased horses (52.4 +/- 1.6%), as detected by E rosettes, was significantly (p less than 0.01) higher than in control animals (42.4 +/- 3.5%). In clinically healthy horses 8.9 +/- 1.1% of PBL were identified by A rosettes as active T cells, whereas animals with a chronic form of EIA had a much lower (p less than 0.001) percentage of these cells (4.7 +/- 0.7%). In the B lymphocyte subpopulations the percentages of cells bearing Fc and C3b receptors were markedly elevated (p less than 0.001) in EIA-infected horses (24.7 +/- 0.8% and 42.8 +/- 2.2% respectively) as compared to uninfected animals (15.1 +/- 1.4% and 29.6 +/- 1.2% respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1989-01-01 PubMed ID: 2788955DOI: 10.1007/BF00366853Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article investigates the percentage of T and B lymphocytes, types of white blood cells that play an essential role in the immune system, in the blood of horses chronically infected with the Equine Infectious Anaemia (EIA) virus. The study discovered significant differences in T and B lymphocyte percentages between uninfected horses and those with long-term EIA infection.
Study Methodology
- The research began by determining the concentrations of T and B lymphocytes, crucial components of the immune response system, in the peripheral blood of both uninfected and EIA-infected horses. Peripheral blood lymphocytes (PBL) refer to lymphocytes found in the bloodstream rather than lymphoid tissue.
- Cells with membrane receptors for sheep erythrocytes, such as T and active T lymphocytes, were identified by E (sheep erythrocyte) and A (active T cell) rosette techniques. A rosette test is an assay that identifies specific cells by their ability to form a rosette, or circular formation, around foreign cells or particles.
- Simultaneously, the detection of B lymphocytes, cells bearing receptors for the C3b component of complement and those with receptors for mouse erythrocytes, was executed using the EAC (sheep erythrocyte, antibody, complement) rosette method.
- The research also profiled the percentage of Fe positive cells by employing the EA rosette test.
Key Findings
- T lymphocytes in the peripheral blood of horses suffering from EIA were significantly higher (52.4 +/- 1.6%), as determined by E rosettes, in comparison to control animals (42.4 +/- 3.5%).
- While 8.9 +/- 1.1% of PBL in clinically healthy horses were marked as active T cells by A rosettes, EIA-afflicted horses showed a significantly lower percentage (4.7 +/- 0.7%) of these cells.
- Marked elevations in the percentages of B cells with Fc and C3b receptors were noted in EIA-infected horses (24.7 +/- 0.8% and 42.8 +/- 2.2% respectively) when compared to uninfected ones (15.1 +/- 1.4% and 29.6 +/- 1.2% respectively).
In summary, the researchers found specific changes in the immune cell profiles of horses affected by EIA, which may underpin immune responses and disease progression in these animals. This research aids understanding of the equine immune system’s response to chronic EIA infection, shedding light on possible intervention strategies.
Cite This Article
APA
Valpotić I, Kastelan M, Rudolf M, Gerencer M, Jukić B, Basić I.
(1989).
T and B lymphocytes in horses persistently infected with equine infectious anaemia virus.
Vet Res Commun, 13(1), 57-65.
https://doi.org/10.1007/BF00366853 Publication
Researcher Affiliations
- Veterinary Faculty, University of Zagreb, Yugoslavia.
MeSH Terms
- Animals
- B-Lymphocytes
- Equine Infectious Anemia / blood
- Equine Infectious Anemia / immunology
- Female
- Horses
- Male
- Rosette Formation
- T-Lymphocytes
References
This article includes 13 references
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