Technical note: a novel method for routine genotyping of horse coat color gene polymorphisms.

The research article details a new, efficient, and cost-effective technique for habitual genotyping of genetic variations that cause most horse coat color differences via real-time PCR methods.

Objective of Research

• The study’s primary objective is to outline a novel, dependable, quick, and cost-effective real-time PCR (Polymerase Chain Reaction) method for routine genotyping of mutations that results in most of the horse coat color variations.

Methodology

• Genetic markers responsible for coat color in horses, namely the melanocortin-1 receptor, Agouti-signaling peptide, and membrane-associated transporter protein alleles were determined simultaneously through the use of two PCR protocols.
• The newly devised test presents itself as an alternative way to genotypically typecast a predefined set of polymorphisms.
• All variations in alleles are detected in real-time on a ‘live’ basis, which eradicates the necessity for post-PCR manipulations, cutting costs and limiting the possibility of cross contamination in the process.
• To facilitate semiautomatic determination of genotype, the data obtained can be transferred to a Microsoft Excel spreadsheet using a freely accessible macro at the provided link.

Application and Results

• The researchers used this new method on a sample set of 156 Spanish Purebred horses, with the aim of demonstrating the effectiveness and applicability of the method in practical scenarios.

Conclusion

• The noted technique presents a new avenue for genetic researchers working on animals, here specifically horses, in their endeavor to efficiently and economically typify gene polymorphism.