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Animal reproduction science2000; 60-61; 493-509; doi: 10.1016/s0378-4320(00)00115-9

Techniques for evaluating selected reproductive disorders of stallions.

Abstract: Numerous techniques may be used for evaluation of the different reproductive disorders of the stallion. Approaches may vary from real-time ultrasonography and biopsy for evaluating testicular tumors to use of special assays for evaluating sperm or plasma for presence of antisperm antibodies. This communication addresses techniques used to evaluate five relatively uncommon, but perplexing, disorders of breeding stallions: (1) seminal vesiculitis, (2) hemospermia associated with idiopathic urethral defects, (3) acrosomal dysfunction, (4) abnormal spermatozoal chromatin, and (5) azoospermia.
Publication Date: 2000-06-14 PubMed ID: 10844219DOI: 10.1016/s0378-4320(00)00115-9Google Scholar: Lookup
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Summary

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This research article discusses various methods that can be used to assess five specific, yet relatively rare and puzzling, reproductive disorders in stallions: seminal vesiculitis, hemospermia, acrosomal dysfunction, abnormal spermatozoal chromatin, and azoospermia.

Overview of the Study

The paper explores several techniques that can be employed to examine various reproductive issues in stallions. The disorders under discussion are selected based on their rarity and the challenges they pose in terms of diagnosis and management. The methods discussed range from real-time ultrasonography and biopsy for the evaluation of testicular tumors, to special assay tests for examining the presence of sperm or antisperm antibodies in the sperm or plasma.

Evaluation Techniques for Reproductive Disorders

There are a number of techniques that can be used to evaluate reproductive disorders:

  • For the diagnosis of testicular tumors, real-time ultrasonography and biopsy are the preferred techniques. These methods provide detailed insight into the structure and composition of the tumors, enabling targeted interventions.
  • For issues related to the presence of antisperm antibodies in the sperm or plasma, specialized assays are the technique of choice. These tests are designed to detect antibodies that might impact the fertility of the stallion.

Five Puzzling Disorders of Breeding Stallions

The paper focuses on following five reproductive disorders in stallions:

  • Seminal vesiculitis: An inflammation of the seminal vesicle, often resulting in decreased fertility. The study explores techniques to diagnose and manage this condition.
  • Hemospermia associated with idiopathic urethral defects: This refers to the presence of blood in sperm, which can often be traced back to unexplained defects in the urethra. The study discusses ways to identify and address these defects.
  • Acrosomal dysfunction: This disorder pertains to functional issues with the acrosome, a cap-like structure on the sperm that aids in the process of fertilization. The article presents methods to assess acrosomal functionality and recommend interventions.
  • Abnormal spermatozoal chromatin: This involves issues with the DNA packaging within the sperm cells. Unable to fertilize, such sperm can lead to infertility. This study outlines techniques for diagnosing and managing this condition.
  • Azoospermia: This is a condition where no sperm are present in the semen. Techniques to detect and manage this disorder are also discussed in the paper.

The article presents an important resource for professionals dealing with equine reproductive health, providing guidance on diagnosing and managing challenging and less common disorders.

Cite This Article

APA
Varner DD, Blanchard TL, Brinsko SP, Love CC, Taylor TS, Johnson L. (2000). Techniques for evaluating selected reproductive disorders of stallions. Anim Reprod Sci, 60-61, 493-509. https://doi.org/10.1016/s0378-4320(00)00115-9

Publication

ISSN: 0378-4320
NlmUniqueID: 7807205
Country: Netherlands
Language: English
Volume: 60-61
Pages: 493-509

Researcher Affiliations

Varner, D D
  • Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station 7784-4475, USA.
Blanchard, T L
    Brinsko, S P
      Love, C C
        Taylor, T S
          Johnson, L

            MeSH Terms

            • Animals
            • Horse Diseases / physiopathology
            • Horses
            • Infertility, Male / physiopathology
            • Infertility, Male / veterinary
            • Male
            • Testicular Neoplasms / diagnostic imaging
            • Testicular Neoplasms / veterinary
            • Ultrasonography

            Citations

            This article has been cited 5 times.
            1. Cruz A, Sullivan DB, Doty KF, Hess RA, Canisso IF, Reddi PP. Acrosomal marker SP-10 (gene name Acrv1) for staging of the cycle of seminiferous epithelium in the stallion. Theriogenology 2020 Oct 15;156:214-221.
            2. Das PJ, McCarthy F, Vishnoi M, Paria N, Gresham C, Li G, Kachroo P, Sudderth AK, Teague S, Love CC, Varner DD, Chowdhary BP, Raudsepp T. Stallion sperm transcriptome comprises functionally coherent coding and regulatory RNAs as revealed by microarray analysis and RNA-seq. PLoS One 2013;8(2):e56535.
              doi: 10.1371/journal.pone.0056535pubmed: 23409192google scholar: lookup
            3. Raudsepp T, McCue ME, Das PJ, Dobson L, Vishnoi M, Fritz KL, Schaefer R, Rendahl AK, Derr JN, Love CC, Varner DD, Chowdhary BP. Genome-wide association study implicates testis-sperm specific FKBP6 as a susceptibility locus for impaired acrosome reaction in stallions. PLoS Genet 2012;8(12):e1003139.
              doi: 10.1371/journal.pgen.1003139pubmed: 23284302google scholar: lookup
            4. Hardefeldt L, Thomas K, Page S, Norris J, Browning G, El Hage C, Stewart A, Gilkerson J, Muscatello G, Verwilghen D, van Galen G, Bauquier J, Cuming R, Reynolds B, Whittaker C, Wilkes E, Clulow J, Burden C, Begg L. Antimicrobial prescribing guidelines for horses in Australia. Aust Vet J 2025 Dec;103(12):781-889.
              doi: 10.1111/avj.70003pubmed: 40903020google scholar: lookup
            5. Bucci R, Parrillo S, Probo M, Varasano V, Romano A, Carluccio A. Case Report: Reproductive evaluation of a Murgese stallion with obstructive azoospermia, accumulation of hyaline material in the ampullae ducts, and corpora amylacea in vesicular glands. Front Vet Sci 2025;12:1571637.
              doi: 10.3389/fvets.2025.1571637pubmed: 40290475google scholar: lookup