Temporal effects of freezing on plasma nitric oxide concentrations in ponies.
Abstract: The purpose of this study was to compare concentrations of nitric oxide (NO) in fresh plasma versus frozen plasma, and determine the temporal effects of freezing on jugular venous plasma NO concentrations in clinically healthy ponies. Twenty-eight helminth-naive ponies, aged from 4 to 6 mo, were raised and maintained under parasite-free conditions. Blood was collected from the jugular vein, centrifuged, and the plasma supernatant was analyzed fresh for NO concentrations using a chemiluminescent method. The remaining samples were aliquoted into 12 samples and stored at -70 degrees C until they were analyzed for NO concentration. Plasma NO concentration was measured at monthly intervals for 12 mo. There were significant differences in the plasma NO concentration across time compared with the baseline value at 1, 3, 4, 6, 7, and 11 mo. However, these values remained within the range for clinically healthy equids compared with concentrations of NO from horses with increased NO from diseased states.
Publication Date: 2003-01-17 PubMed ID: 12528834PubMed Central: PMC227032
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research assesses how freezing affects the nitric oxide (NO) levels in a pony’s plasma over time. Results indicate that while freezing can significantly alter the NO concentration, the levels generally remained within the range typical for healthy equids.
Research Purposes and Methodology
- This research aimed to investigate how freezing impacts nitric oxide (NO) concentrations in the plasma of healthy ponies, and to compare these concentrations to those in fresh plasma.
- The ponies selected were helminth-naive (meaning they’d never been infected with parasitic worms), aged between 4 and 6 months, and kept under parasite-free conditions.
- Blood was collected from the jugular veins of 28 ponies, centrifuged, and the resulting plasma supernatant was analyzed immediately (fresh) for nitric oxide concentration using a chemiluminescent method, which is a technique that measures the light emitted as a result of a chemical reaction.
- The residual samples were divided into 12 portions and frozen at -70 degrees Celsius to be analyzed for NO concentration at a later date.
Findings of the Study
- Plasma NO concentration was evaluated at monthly intervals for a year.
- There were significant differences in plasma NO concentrations over time compared to the initial (baseline) value at 1, 3, 4, 6, 7, and 11 months.
- Despite these differences, the NO levels remained within the normal range for healthy equids, when compared to NO concentrations from horses with diseases that cause increased NO.
Implications of the Results
- The fluctuations in NO levels – as influenced by freezing – suggest that for accurate nitric oxide measurement, it would be ideal to analyze fresh samples rather than frozen ones.
- These findings offer valuable insights into the impact of preservation methods on biological samples, which could be instrumental in future research and diagnostic procedures involving equids or other species.
Cite This Article
APA
Hubert JD, Seahorn TL, Klei TR, Hosgood G, Moore RM.
(2003).
Temporal effects of freezing on plasma nitric oxide concentrations in ponies.
Can J Vet Res, 67(1), 72-74.
Publication
Researcher Affiliations
- Equine Studies Health Program, Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana 70803-8410, USA. jhubert@vetmed.lsu.edu
MeSH Terms
- Animals
- Blood Preservation / methods
- Blood Preservation / standards
- Blood Preservation / veterinary
- Female
- Freezing
- Horse Diseases / blood
- Horses / blood
- Male
- Nitric Oxide / blood
- Specimen Handling / methods
- Specimen Handling / veterinary
- Time Factors
References
This article includes 14 references
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Citations
This article has been cited 1 times.- Mirza MH, Seahorn TL, Oliver JL, Hosgood G, Moore RM. Detection and comparison of nitric oxide in clinically healthy horses and those with naturally acquired strangulating large colon volvulus. Can J Vet Res 2005 Apr;69(2):106-15.
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