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Journal of equine science2013; 24(2); 17-24; doi: 10.1294/jes.24.17

Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration.

Abstract: In vitro cell studies might be a useful tool for studying tendon pathology, but no suitable in vitro models exist for tendon disorders. The purpose of this study was to confirm whether cell scratch culture using tendon-derived fibroblasts can provide a suitable in vitro tendon disorder model. Extracellular matrix components were examined immunohistochemically in tendon tissue, and then their related gene expression levels were analyzed by conventional reverse transcription polymerase chain reaction (RT-PCR) and/or quantitative real-time RT-PCR in tissues and cells. Collagen type I (Col I), collagen type III (Col III), tenascin-C (TN-C) and cartilage oligomeric matrix protein (COMP) were detected in tendon tissue sections, and RT-PCR confirmed their expression in tendon tissue and cells. Cells that had been cultured from explanted tendon tissue maintained the characteristics of in vivo tendon cells. The combination of TN-C and COMP might be a useful marker of tendon cells because they display more tendon-specific expression than Col I and III. In particular, the significant increase of TN-C mRNA expression in the scratch wound assay, at 12 hr after scratching, concomitant with the regeneration of the cell sheet, indicates its crucial role in tendon cell proliferation and migration. Thus, TN-C appears to be a key factor in tendon wound healing. In vitro cell scratch assays using tendon cells appear to mimic the repair of tendon tissue after injury.
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Publication Date: 2013-06-28 PubMed ID: 24833997PubMed Central: PMC4013982DOI: 10.1294/jes.24.17Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores a potential method to create an in vitro model of tendon disorders using tissue derived from tendons. The researchers looked specifically at the extracellular matrix components and found that tenascin-C, a glycoprotein in the extracellular matrix, could be vital in tendon cell proliferation and migration, and thus helpful in tendon wound healing.

Objectives and Purpose of the Study

  • The purpose of the study was to discover whether using tendon-derived fibroblasts in cell scratch culture could serve as a suitable in vitro model for tendon disorders.
  • The research was motivated by the lack of existing in vitro models for studying tendon pathology.

Approach and Methodology

  • The study analyzed extracellular matrix components in tendon tissue both immunohistochemically and at the gene expression level.
  • They looked specifically at collagen type I (Col I), collagen type III (Col III), tenascin-C (TN-C) and cartilage oligomeric matrix protein (COMP) in the tendon tissue sections.
  • The research utilized conventional reverse transcription polymerase chain reaction (RT-PCR) and/or quantitative real-time RT-PCR to check the expression of the aforementioned components in tissues and cells.
  • It also conducted a cell scratch wound assay and noted changes in TN-C mRNA expressions over time.

Results

  • The study confirmed that cells cultured from explanted tendon tissue maintain characteristics akin to in vivo tendon cells.
  • The researchers deduced that the combination of TN-C and COMP can be useful markers of tendon cells because their expression is more specific to tendons than that of Collagen types I and III.
  • Notably, the study found a significant increase in TN-C mRNA expression 12 hours following the scratch wound assay, coinciding with the cell sheet’s regeneration. This suggests TN-C’s critical role in tendon cell proliferation and migration.

Conclusions and Implications

  • The finding suggests that TN-C may be a key player in the healing of tendon wounds.
  • The in vitro cell scratch assays using tendon cells were found to mimic tendon tissue repair after injury, presenting a promising potential model for studying tendon disorders.

Cite This Article

APA
Nemoto M, Kizaki K, Yamamoto Y, Oonuma T, Hashizume K. (2013). Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration. J Equine Sci, 24(2), 17-24. https://doi.org/10.1294/jes.24.17

Publication

Journal of equine science
ISSN: 1340-3516
NlmUniqueID: 9503751
Country: Japan
Language: English
Volume: 24
Issue: 2
Pages: 17-24

Researcher Affiliations

Nemoto, Manabu
  • Laboratory of Veterinary Physiology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan ; Present address: Epizootic Research Center, Equine Research Institute, Japan Racing Association, Shiba 1400-4, Shimotsuke, Tochigi 329-0412, Japan.
Kizaki, Keiichiro
  • Laboratory of Veterinary Physiology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
Yamamoto, Yoshio
  • Laboratory of Veterinary Biochemistry and Cell Biology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
Oonuma, Toshina
  • Laboratory of Veterinary Physiology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan ; Present address: Department of Biological Resources, Integrated Center for Sciences, Ehime University, Shitsukawa, Toon, Ehime 791-0295, Japan.
Hashizume, Kazuyoshi
  • Laboratory of Veterinary Physiology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.

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Citations

This article has been cited 5 times.
  1. Inguito KL, Schofield MM, Faghri AD, Bloom ET, Heino M, West VC, Ebron KMM, Elliott DM, Parreno J. Stress deprivation of tendon explants or Tpm3.1 inhibition in tendon cells reduces F-actin to promote a tendinosis-like phenotype. Mol Biol Cell 2022 Dec 1;33(14):ar141.
    doi: 10.1091/mbc.E22-02-0067pubmed: 36129771google scholar: lookup
  2. Mirghaderi SP, Valizadeh Z, Shadman K, Lafosse T, Oryadi-Zanjani L, Yekaninejad MS, Nabian MH. Cell therapy efficacy and safety in treating tendon disorders: a systemic review of clinical studies. J Exp Orthop 2022 Aug 30;9(1):85.
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  3. Cho WS, Chung SG, Kim W, Jo CH, Lee SU, Lee SY. Mesenchymal Stem Cells Use in the Treatment of Tendon Disorders: A Systematic Review and Meta-Analysis of Prospective Clinical Studies. Ann Rehabil Med 2021 Aug;45(4):274-283.
    doi: 10.5535/arm.21078pubmed: 34496470google scholar: lookup
  4. Antoniel M, Traina F, Merlini L, Andrenacci D, Tigani D, Santi S, Cenni V, Sabatelli P, Faldini C, Squarzoni S. Tendon Extracellular Matrix Remodeling and Defective Cell Polarization in the Presence of Collagen VI Mutations. Cells 2020 Feb 11;9(2).
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  5. Sardone F, Traina F, Bondi A, Merlini L, Santi S, Maraldi NM, Faldini C, Sabatelli P. Tendon Extracellular Matrix Alterations in Ullrich Congenital Muscular Dystrophy. Front Aging Neurosci 2016;8:131.
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