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Home / Equine Research Bank / Int J Artif Organs / Tenogenic differentiation of equine mesenchymal progenitor c...
The International journal of artificial organs2012; 35(11); 996-1005; doi: 10.5301/ijao.5000129

Tenogenic differentiation of equine mesenchymal progenitor cells under indirect co-culture.

Abstract: Adult bone marrow mesenchymal stem cells (BM-MSCs) are a potential cell source for tendon repair in direct cell therapy and tissue engineering investigations. The purpose of this study was to evaluate the tenogenic induction of undifferentiated BM-MSCs under indirect co-culture technique with trimmed native tendon tissue. Since the horse represents a preferred species to study tendon regenerative strategies, this work was conducted on equine BM-MSCs. Methods: Equine BM-MSCs were co-cultured in a transwell system with tendon tissue fragments. The BM-MSC tenogenic differentiation was evaluated by cytochemical staining and real time PCR for gene expression. Cell viability in tendon fragments and cultured cells was analyzed. Results: Our results indicate that under indirect co-culture with native and healthy tendon tissue the BM-MSCs expressed tendon-specific markers such as decorin, tenomodulin, tenascin-C, and collagen type I. They also retained a tenocyte-like phenotype during monolayer culture. Conclusions: Data are very encouraging for future in vitro investigations into committing cells to the tenogenic lineage without adding growth factors or serum to the culture medium for both cell therapy and tissue engineering.
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Publication Date: 2012-10-16 PubMed ID: 23065882DOI: 10.5301/ijao.5000129Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study examined the potential of adult bone marrow mesenchymal stem cells in inducing tendon repair. It found that when these cells are co-cultured with native tendon tissue, they show an increased expression of tendon-specific markers, suggesting a possible future application in the field of tendon regeneration.

Objective of the Research

  • The primary aim was to evaluate the tenogenic induction (a process where cells are differentiated to form tendon-like tissue) of undifferentiated bone marrow mesenchymal stem cells (BM-MSCs).
  • The researchers used an indirect co-culture technique, wherein the BM-MSCs were grown together with trimmed native tendon tissue in a controlled environment.
  • The study specifically focused on equine BM-MSCs because horses are considered a preferred species for studying tendon regenerative strategies due to the similarities in tendon anatomy and characteristics with humans.

Methodology

  • Equine BM-MSCs were co-cultured in a transwell system with tendon tissue fragments. A transwell system is a device used in laboratories to grow cells in a controlled environment.
  • The tenogenic differentiation of BM-MSCs was then evaluated by monitoring the gene expression with real-time PCR (Polymerase Chain Reaction) and cytochemical staining, a technique used to visualize specific cellular components.
  • The tendon-specific markers that were analyzed included decorin, tenomodulin, tenascin-C, and collagen type I.

Results

  • The study found that BM-MSCs, when indirectly co-cultured with healthy and native tendon tissue, expressed increased levels of tendon-specific markers, suggesting a transformation into a tenocyte-like phenotype.
  • The tendon-like cells were able to maintain their phenotype during monolayer culture, which is a standard technique of growing cells in a laboratory.

Conclusion

  • The findings suggest a promising potential for inducing tenogenic differentiation without the need for additional growth factors or serum in the culture medium.
  • These results are promising for future in vitro (outside the living body) investigations into directing cells towards tenogenic lineage. This could significantly contribute to both cell therapy and tissue engineering efforts for tendon repair.

Cite This Article

APA
Lovati AB, Corradetti B, Cremonesi F, Bizzaro D, Consiglio AL. (2012). Tenogenic differentiation of equine mesenchymal progenitor cells under indirect co-culture. Int J Artif Organs, 35(11), 996-1005. https://doi.org/10.5301/ijao.5000129

Publication

The International journal of artificial organs
ISSN: 1724-6040
NlmUniqueID: 7802649
Country: United States
Language: English
Volume: 35
Issue: 11
Pages: 996-1005

Researcher Affiliations

Lovati, Arianna B
  • Reproduction Unit, Department of Veterinary Clinical Science, University of Milan, Lodi, Italy. arianna.lovati@grupposandonato.it
Corradetti, Bruna
    Cremonesi, Fausto
      Bizzaro, Davide
        Consiglio, Anna Lange

          MeSH Terms

          • Animals
          • Cell Culture Techniques
          • Cell Differentiation / physiology
          • Cell Survival / physiology
          • Coculture Techniques
          • Collagen Type I / genetics
          • Collagen Type I / metabolism
          • Decorin / genetics
          • Decorin / metabolism
          • Horses
          • Mesenchymal Stem Cells / cytology
          • RNA, Messenger / metabolism
          • Tenascin / genetics
          • Tenascin / metabolism
          • Tendons / cytology
          • Tissue Engineering

          Citations

          This article has been cited 17 times.
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