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The absence of ER-β results in altered gene expression in ovarian granulosa cells isolated from in vivo preovulatory follicles.
Abstract: Determining the spatial and temporal expression of genes involved in the ovulatory pathway is critical for the understanding of the role of each estrogen receptor in the modulation of folliculogenesis and ovulation. Estrogen receptor (ER)-β is highly expressed in ovarian granulosa cells, and mice lacking ER-β are subfertile due to inefficient ovulation. Previous work has focused on isolated granulosa cells or cultured follicles and, although informative, provides confounding results due to the heterogeneous cell types present including granulosa and theca cells and oocytes and exposure to in vitro conditions. Herein we isolated preovulatory granulosa cells from wild-type (WT) and ERβ-null mice using laser capture microdissection to examine the genomic transcriptional response downstream of pregnant mare serum gonadotropin (mimicking FSH) and pregnant mare serum gonadotropin/human chorionic gonadotropin (mimicking LH) stimulation. This allows for a direct comparison of in vivo granulosa cells at the same stage of development from both WT and ERβ-null ovaries. ERβ-null granulosa cells showed altered expression of genes known to be regulated by FSH (Akap12 and Runx2) as well as not previously reported (Arnt2 and Pou5f1) in WT granulosa cells. Our analysis also identified 304 genes not previously associated with ERβ in granulosa cells. LH-responsive genes including Abcb1b and Fam110c show reduced expression in ERβ-null granulosa cells; however, novel genes including Rassf2 and Megf10 were also identified as being downstream of LH signaling in granulosa cells. Collectively, our data suggest that granulosa cells from ERβ-null ovaries may not be appropriately differentiated and are unable to respond properly to gonadotropin stimulation.
Publication Date: 2013-04-11 PubMed ID: 23580569PubMed Central: PMC3740481DOI: 10.1210/en.2012-2256Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- N.I.H.
- Intramural
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research investigated the role of estrogen receptor (ER)β in the expression of specific genes in ovarian granulosa cells, vital for the ovulatory process. The study found that the absence of ERβ alters the expression of genes related to folliculogenesis and ovulation, potentially impeding the cells’ ability to respond to gonadotropin stimulation.
Methodology
- The study utilized granulosa cells isolated from the ovaries of both wild-type (WT) and ERβ-null (without ERβ) mice.
- These cells were isolated using laser capture microdissection, allowing for precise selection of cell types, and reducing confounding factors that previous studies might have encountered due to mixing of cell types or in vitro conditions.
- The granulosa cells were stimulated with pregnant mare serum gonadotropin, which mimics follicle-stimulating hormone (FSH), and a combination of this with human chorionic gonadotropin (mimicking luteinizing hormone, LH).
Findings
- The research found that the absence of ERβ in granulosa cells alters the expression of several genes. Some of these altered genes are known to be regulated by FSH, such as Akap12 and Runx2, while others, including Arnt2 and Pou5f1, hadn’t been previously reported in WT granulosa cells.
- The study also identified over 300 genes associated with ERβ.
- LH-responsive genes, like Abcb1b and Fam110c, were found to have reduced expression in ERβ-null granulosa cells. Nonetheless, novel genes, such as Rassf2 and Megf10, were also identified as being impacted by LH in granulosa cells.
- These results indicate that ERβ-null granulosa cells might not be appropriately differentiated, and may not respond properly to gonadotropin stimulation. This could lead to issues with ovulation, and further, to subfertility.
Conclusion
- This research provides a clearer depiction of the importance of ERβ in folliculogenesis and ovulation by mapping the genes influenced by this receptor.
- It also gives leads for future research, especially concerning the novel genes identified during the study.
- The understanding of how the absence of ERβ impacts gene expression could shed light on fertility issues and can potentially lead to effective therapeutic interventions for subfertility.
Cite This Article
APA
Binder AK, Rodriguez KF, Hamilton KJ, Stockton PS, Reed CE, Korach KS.
(2013).
The absence of ER-β results in altered gene expression in ovarian granulosa cells isolated from in vivo preovulatory follicles.
Endocrinology, 154(6), 2174-2187.
https://doi.org/10.1210/en.2012-2256 Publication
Researcher Affiliations
- National Institute of Environmental Health Sciences, Laboratory of Reproduction and Developmental Toxicology, 111 TW Alexander Drive, MD B3-02, Research Triangle Park, North Carolina 27709, USA.
MeSH Terms
- Animals
- Cells, Cultured
- Chorionic Gonadotropin / pharmacology
- Estrogen Receptor beta / deficiency
- Estrogen Receptor beta / genetics
- Female
- Gene Expression Profiling
- Gonadotropins, Equine / pharmacology
- Granulosa Cells / drug effects
- Granulosa Cells / metabolism
- Horses
- Humans
- Mice
- Mice, Knockout
- Oligonucleotide Array Sequence Analysis
- Ovarian Follicle / cytology
- Ovarian Follicle / metabolism
- Ovary / cytology
- Ovary / metabolism
- Ovulation / genetics
- Pregnancy
- Time Factors
Grant Funding
- Z01 ES070065 / Intramural NIH HHS
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