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Home / Equine Research Bank / Immunology / The characterization of equine herpes virus-1-infected cell ...
Immunology1988; 63(2); 193-198;

The characterization of equine herpes virus-1-infected cell polypeptides recognized by equine lymphocytes.

Abstract: Ponies, without evidence of previous exposure to Equine herpes virus-1 (EHV-1), were experimentally infected with EHV-1 subtype 2 and investigated for lymphocyte transformation to virus-infected cell polypeptides, as shown by separation with gel electrophoresis. Animals made significant responses to Western blot fractions that corresponded to molecular weights of approximately 30,000, 40,000-45,000, 60,000-65,000, 80,000-95,000 and 100,000-140,000 MW. These molecular weight ranges correlated with the positions of major EHV-1 subtype 2 glycoproteins that were found at migration distances approximating to 137,000, 111,000, 90,000, 65,000 and 47,000 MW. Responses were also made to a subset of similar points on the subtype 1 profile. Hyperimmune equine serum precipitated numerous infected-cell proteins of both subtypes; in particular the recognition of polypeptides with MW of 142,000, 132,000, 114,000, and 46,000 was in agreement with the mitogenic responses. Labelling with 125I indicated that immunoprecipitated greater than 250,000, 182,000, 142,000, 132,000, 75,000, 46,000 and 32,000/34,000 MW products were exposed on the surface of infected cells.
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Publication Date: 1988-02-01 PubMed ID: 2832309PubMed Central: PMC1454525
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper focuses on understanding the response of ponies, with no prior exposure to Equine Herpes Virus-1 (EHV-1), to an experimental EHV-1 subtype 2 infection, analyzing the response of their lymphocytes to the virus-infected cell polypeptides.

Research Methodology

  • The study designs an experimental scenario where EHV-1 naive ponies are infected with EHV-1 subtype 2.
  • The lymphocyte transformations related to virus-infected cell polypeptides are investigated by separating them using gel electrophoresis.
  • The significant responses from animals are noted with regards to Western blot fractions. This corresponds to the molecular weights of specific ranges – around 30,000, 40,000-45,000, 60,000-65,000, 80,000-95,000, and 100,000-140,000 MW.
  • The researchers also observed these molecular weight ranges in relation to major EHV-1 subtype 2 glycoproteins discovered at specific migration distances, similar to the previously mentioned molecular weights.

Findings

  • Research presents findings that reveal animals reacted to a subset of points on the subtype 1 profile, highlighting the similarities in response to subtype 2.
  • Hyperimmune equine serum identified numerous infected-cell proteins within both subtypes, specifically recognizing polypeptides with MW (molecular weight) of 142,000, 132,000, 114,000, and 46,000, which aligns with the observed mitogenic responses.
  • An application of 125I labeling disclosed that immunoprecipitated products with molecular weights exceeding 250,000, 182,000, 142,000, 132,000, 75,000, 46,000, and 32,000/34,000 MW are presented on the surface of infected cells.

Conclusion

  • The study provides valuable insights into the cellular responses in ponies when exposed to EHV-1 subtype 2 infection. It shows the transformation and interaction of lymphocytes with virus-infected cell polypeptides among these animals.
  • Significant responses from the animals were noted and correlated with specific molecular weights associated with EHV-1 subtype 2 glycoproteins.
  • Through the data captured, the paper provides a discernible pathway to understand how EHV-1 subtype 2 infects hosts, and the potential similarities or differences from EHV-1 subtype 1.

Cite This Article

APA
Bridges CG, Ledger N, Edington N. (1988). The characterization of equine herpes virus-1-infected cell polypeptides recognized by equine lymphocytes. Immunology, 63(2), 193-198.

Publication

Immunology
ISSN: 0019-2805
NlmUniqueID: 0374672
Country: England
Language: English
Volume: 63
Issue: 2
Pages: 193-198

Researcher Affiliations

Bridges, C G
  • Department of Microbiology and Parasitology, Royal Veterinary College, London, U.K.
Ledger, N
    Edington, N

      MeSH Terms

      • Animals
      • Antigens, Viral / immunology
      • Electrophoresis, Polyacrylamide Gel
      • Glycoproteins / analysis
      • Herpesviridae / immunology
      • Herpesviridae Infections / immunology
      • Herpesvirus 1, Equid / immunology
      • Horses
      • Lymphocyte Activation
      • Mitosis
      • Molecular Weight
      • Viral Proteins / analysis

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      Citations

      This article has been cited 1 times.
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        doi: 10.1007/BF01314651pubmed: 2458087google scholar: lookup
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