The combination of purified recombinant human platelet-derived growth factor-BB and equine particulate bone graft for periodontal regeneration.
Abstract: The objective of this study is to evaluate the potential for periodontal regeneration of a critical-sized defect with the application of recombinant human platelet-derived growth factor (rhPDGF-BB) combined with either a particulate equine or a β-tricalcium phosphate (β-TCP) matrix. Methods: Critical-sized intrabony 2-wall defects were created bilaterally on the distal surface of the second premolar and the mesial surface of the first molar in nine hounds. Twelve defects received rhPDGF-BB/equine treatment, 12 defects received rhPDGF-BB/β-TCP treatment, and the remaining 12 sites served as sham-surgery controls. The animals were sacrificed after a 10-week healing period. Results: Clinical healing was uneventful without obvious signs of overt gingival inflammation. Histologic and histomorphometric analyses revealed statistically that there were differences among the three groups in terms of new bone formation (P <0.001). The amount of test material for both rhPDGF-BB/equine and rhPDGF-BB/β-TCP groups was comparable, but the amount of newly formed bone was significantly higher (P <0.01) in favor of the rhPDGF-BB/equine group. The amount of new cementum formed for the rhPDGF-BB/equine group (4.8 ± 1.3 mm) was significantly higher (P =0.001) than the sham-surgery control group (1.7 ± 1.9 mm). Conclusions: Both rhPDGF-BB/equine and rhPDGF-BB/β-TCP have the potential to support the regeneration of the periodontal attachment apparatus.
Publication Date: 2011-10-20 PubMed ID: 22014175DOI: 10.1902/jop.2011.110298Google Scholar: Lookup
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- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research evaluates the effectiveness of human platelet-derived growth factor in combination with a compound derived from equine bone or a phosphorus-calcium compound in regenerating periodontal tissue in dogs with artificially created dental defects.
Objective and Methodology
- The study aimed to investigate the potential of two different treatments for periodontal regeneration. The treatments involved the use of a protein, called recombinant human platelet-derived growth factor (rhPDGF-BB), in combination with either a particle of equine bone or a β-tricalcium phosphate (β-TCP) matrix.
- For the study, specific dental defects (intrabony 2-wall defects) were created on the distal surface of the second premolar and the mesial surface of the first molar in nine hounds.
- The dogs were then divided into three groups – one group treated with rhPDGF-BB combined with equine bone (12 defects), another group treated with rhPDGF-BB combined with β-TCP (12 defects), and the last group as a control which had sham surgery (12 defects).
- The results were evaluated after a healing period of 10 weeks, after which the animals were sacrificed.
Results
- The study found that both groups that received treatments showed signs of healing without any significant inflammation of the gums.
- Statistical comparison of the three groups showed there were significant differences in new bone formation. The rhPDGF-BB/equine and rhPDGF-BB/β-TCP treatments both showed equivalent amounts of test material, but new bone creation was significantly higher in the group that used the equine-based treatment.
- The rhPDGF-BB/equine group showed a significantly larger amount of new cementum (the tissue that covers the root of a tooth) when compared to the control group.
Conclusions
- In conclusion, this study indicates that both the rhPDGF-BB/equine and rhPDGF-BB/β-TCP treatments have potential in supporting the regeneration of periodontal tissue.
- The rhPDGF-BB/equine treatment appeared to hold a greater benefit in terms of new bone and cementum formation.
Cite This Article
APA
Nevins M, Nevins ML, Karimbux N, Kim SW, Schupbach P, Kim DM.
(2011).
The combination of purified recombinant human platelet-derived growth factor-BB and equine particulate bone graft for periodontal regeneration.
J Periodontol, 83(5), 565-573.
https://doi.org/10.1902/jop.2011.110298 Publication
Researcher Affiliations
- Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA, USA.
MeSH Terms
- Animals
- Becaplermin
- Bone Matrix / transplantation
- Bone Regeneration / drug effects
- Bone Substitutes / pharmacology
- Bone Transplantation
- Calcium Phosphates / pharmacology
- Dental Cementum / physiology
- Dogs
- Female
- Horses
- Humans
- Periodontal Ligament / physiology
- Proto-Oncogene Proteins c-sis / pharmacology
- Recombinant Proteins / pharmacology
- Regeneration / drug effects
Citations
This article has been cited 5 times.- Boyan BD, Lotz EM, Schwartz Z. (*) Roughness and Hydrophilicity as Osteogenic Biomimetic Surface Properties.. Tissue Eng Part A 2017 Dec;23(23-24):1479-1489.
- Zang SQ, Kang S, Hu X, Wang M, Wang XW, Zhou T, Wang QT. Comparison of Different Periodontal Healing of Critical Size Noncontained and Contained Intrabony Defects in Beagles.. Chin Med J (Engl) 2017 Feb 20;130(4):477-486.
- Fujioka-Kobayashi M, Schaller B, Kobayashi E, Hernandez M, Zhang Y, Miron RJ. Hyaluronic Acid Gel-Based Scaffolds as Potential Carrier for Growth Factors: An In Vitro Bioassay on Its Osteogenic Potential.. J Clin Med 2016 Nov 30;5(12).
- Al-Hezaimi K, Ramalingam S, Al-Askar M, ArRejaie AS, Nooh N, Jawad F, Aldahmash A, Atteya M, Wang CY. Real-time-guided bone regeneration around standardized critical size calvarial defects using bone marrow-derived mesenchymal stem cells and collagen membrane with and without using tricalcium phosphate: an in vivo micro-computed tomographic and histologic experiment in rats.. Int J Oral Sci 2016 Mar 30;8(1):7-15.
- Pellegrini G, Pagni G, Rasperini G. Surgical Approaches Based on Biological Objectives: GTR versus GBR Techniques.. Int J Dent 2013;2013:521547.
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