FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Immunogenetics / The common equine class I molecule Eqca-1*00101 (ELA-A3.1) i...
Immunogenetics2015; 67(11-12); 675-689; doi: 10.1007/s00251-015-0872-z

The common equine class I molecule Eqca-1*00101 (ELA-A3.1) is characterized by narrow peptide binding and T cell epitope repertoires.

Abstract: Here we describe a detailed quantitative peptide-binding motif for the common equine leukocyte antigen (ELA) class I allele Eqca-1*00101, present in roughly 25 % of Thoroughbred horses. We determined a preliminary binding motif by sequencing endogenously bound ligands. Subsequently, a positional scanning combinatorial library (PSCL) was used to further characterize binding specificity and derive a quantitative motif involving aspartic acid in position 2 and hydrophobic residues at the C-terminus. Using this motif, we selected and tested 9- and 10-mer peptides derived from the equine herpesvirus type 1 (EHV-1) proteome for their capacity to bind Eqca-1*00101. PSCL predictions were very efficient, with an receiver operating characteristic (ROC) curve performance of 0.877, and 87 peptides derived from 40 different EHV-1 proteins were identified with affinities of 500 nM or higher. Quantitative analysis revealed that Eqca-1*00101 has a narrow peptide-binding repertoire, in comparison to those of most human, non-human primate, and mouse class I alleles. Peripheral blood mononuclear cells from six EHV-1-infected, or vaccinated but uninfected, Eqca-1*00101-positive horses were used in IFN-γ enzyme-linked immunospot (ELISPOT) assays. When we screened the 87 Eqca-1*00101-binding peptides for T cell reactivity, only one Eqca-1*00101 epitope, derived from the intermediate-early protein ICP4, was identified. Thus, despite its common occurrence in several horse breeds, Eqca-1*00101 is associated with a narrow binding repertoire and a similarly narrow T cell response to an important equine viral pathogen. Intriguingly, these features are shared with other human and macaque major histocompatibility complex (MHC) molecules with a similar specificity for D in position 2 or 3 in their main anchor motif.
Get Full Text
Publication Date: 2015-09-23 PubMed ID: 26399241PubMed Central: PMC4783141DOI: 10.1007/s00251-015-0872-zGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the specificity of the equine leukocyte antigen (ELA) class I allele Eqca-1*00101 in binding with various peptides derived from the equine herpesvirus. It suggests that the allele, found in about 25% of Thoroughbred horses, has a limited binding and T cell response repertoire which may have implications for equine viral diseases.

Research Methodology

  • The researchers first established a preliminary binding motif which characterizes the specific pattern or structure that a certain molecule, in this case the Eqca-1*00101, uses to interact with other molecules. This was accomplished by sequencing the ligands, molecules that bind to the central molecule, endogenously bound to the Eqca-1*00101.
  • Following this, a positional scanning combinatorial library (PSCL) was employed to get finer details about the binding specificity of the Eqca-1*00101 and to derive a quantitative motif. This revealed the importance of aspartic acid in the 2nd position and hydrophobic residues at the C-terminus in the binding activity.

Peptide Binding

  • Using the quantitative motif, the researchers then proceeded to test 9- and 10-mer peptides derived from the equine herpesvirus type 1 (EHV-1) proteome. Here, the PSCL predictions were found to be quite effective, with an receiver operating characteristic (ROC) curve performance of 0.877.
  • 87 peptides in all, originating from 40 different EHV-1 proteins, exhibited binding affinities of 500 nM or higher with Eqca-1*00101 showing that Eqca-1*00101 has a somewhat limited peptide-binding repertoire, when compared to other human, non-human primate, and mouse class I alleles.

T Cell Reactivity and Epitope Identification

  • Peripheral blood mononuclear cells from six EHV-1-infected, or vaccinated but uninfected, Eqca-1*00101-positive horses were used in an interferon gamma (IFN-γ) enzyme-linked immunospot (ELISPOT) assay.
  • From the aforementioned 87 Eqca-1*00101-binding peptides, the assay revealed only one Eqca-1*00101 epitope that came from the intermediate-early protein ICP4. An epitope is the part of an antigen that an antibody attaches itself to.
  • This highlights that in addition to a narrow peptide binding repertoire, Eqca-1*00101 is also associated with a narrow T cell response.

Summary

  • Overall, the study provides an in-depth understanding of how Eqca-1*00101 interacts with, and responds to, certain viral proteins. Similarities in such narrow repertoires in human and macaque major histocompatibility complex (MHC) molecules highlight a potentially significant element of both equine and primate immunology.

Cite This Article

APA
Bergmann T, Moore C, Sidney J, Miller D, Tallmadge R, Harman RM, Oseroff C, Wriston A, Shabanowitz J, Hunt DF, Osterrieder N, Peters B, Antczak DF, Sette A. (2015). The common equine class I molecule Eqca-1*00101 (ELA-A3.1) is characterized by narrow peptide binding and T cell epitope repertoires. Immunogenetics, 67(11-12), 675-689. https://doi.org/10.1007/s00251-015-0872-z

Publication

Immunogenetics
ISSN: 1432-1211
NlmUniqueID: 0420404
Country: United States
Language: English
Volume: 67
Issue: 11-12
Pages: 675-689

Researcher Affiliations

Bergmann, Tobias
  • Institut für Virologie, Freie Universtiät Berlin, 14163, Berlin, Germany.
Moore, Carrie
  • Department of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA, 92037, USA.
Sidney, John
  • Department of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA, 92037, USA.
Miller, Donald
  • Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA.
Tallmadge, Rebecca
  • Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA.
Harman, Rebecca M
  • Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA.
Oseroff, Carla
  • Department of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA, 92037, USA.
Wriston, Amanda
  • Department of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.
Shabanowitz, Jeffrey
  • Department of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.
Hunt, Donald F
  • Department of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA.
  • Department of Pathology, University of Virginia, Charlottesville, VA, 22904, USA.
Osterrieder, Nikolaus
  • Institut für Virologie, Freie Universtiät Berlin, 14163, Berlin, Germany.
Peters, Bjoern
  • Department of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA, 92037, USA.
Antczak, Douglas F
  • Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA.
Sette, Alessandro
  • Department of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA, 92037, USA. alex@lji.org.

MeSH Terms

  • Alleles
  • Animals
  • Epitopes, T-Lymphocyte / immunology
  • Epitopes, T-Lymphocyte / metabolism
  • Herpesviridae Infections / genetics
  • Herpesviridae Infections / immunology
  • Herpesviridae Infections / veterinary
  • Herpesviridae Infections / virology
  • Herpesvirus 1, Equid / genetics
  • Herpesvirus 1, Equid / immunology
  • Histocompatibility Antigens Class I / immunology
  • Histocompatibility Antigens Class I / metabolism
  • Horse Diseases / genetics
  • Horse Diseases / immunology
  • Horse Diseases / virology
  • Horses
  • Humans
  • Leukocytes, Mononuclear
  • Mice
  • Peptide Fragments / immunology
  • Peptide Fragments / metabolism
  • Protein Binding
  • Proteome / immunology
  • T-Lymphocytes, Cytotoxic / immunology
  • T-Lymphocytes, Cytotoxic / metabolism
  • Tandem Mass Spectrometry

Grant Funding

  • U19 AI090023 / NIAID NIH HHS
  • R01 AI112566 / NIAID NIH HHS
  • U19AI090023 / NIAID NIH HHS
  • HHSN272200900042C / PHS HHS
  • HHSN272200900042C / NIAID NIH HHS
  • R37 AI033993 / NIAID NIH HHS
  • HHSN272201400045C / NIAID NIH HHS
  • AI 033993 / NIAID NIH HHS
  • R01AI112566 / NIAID NIH HHS
  • R01 AI033993 / NIAID NIH HHS
  • HHSN272201400045C / PHS HHS

References

This article includes 80 references
  1. Albrecht RA, Jang HK, Kim SK, O'Callaghan DJ. Direct interaction of TFIIB and the IE protein of equine herpesvirus 1 is required for maximal trans-activation function.. Virology 2003 Nov 25;316(2):302-12.
    pubmed: 14644612doi: 10.1016/j.virol.2003.08.017google scholar: lookup
  2. Allen G, Yeargan M, Costa LR, Cross R. Major histocompatibility complex class I-restricted cytotoxic T-lymphocyte responses in horses infected with equine herpesvirus 1.. J Virol 1995 Jan;69(1):606-12.
    pmc: PMC188619pubmed: 7983765doi: 10.1128/jvi.69.1.606-612.1995google scholar: lookup
  3. Allen GP. Risk factors for development of neurologic disease after experimental exposure to equine herpesvirus-1 in horses.. Am J Vet Res 2008 Dec;69(12):1595-600.
    pubmed: 19046006doi: 10.2460/ajvr.69.12.1595google scholar: lookup
  4. Allen GP, Kydd JH, Slater JD, Smith KC. Equid herpesvirus 1 and equid herpesvirus 4 infections. In: Coetzer JAW, Tustin RC, editors. Infectious diseases of livestock. Newmarket: Oxford University Press; 2004.
  5. Ambagala APN, Gopinath RS, Srikumaran S. Peptide transport activity of the transporter associated with antigen processing (TAP) is inhibited by an early protein of equine herpesvirus-1.. J Gen Virol 2004 Feb;85(Pt 2):349-353.
    pubmed: 14769892doi: 10.1099/vir.0.19563-0google scholar: lookup
  6. Antczak DF, Bailey E, Barger B, Guerin G, Lazary S, McClure J, Mottironi VD, Symons R, Templeton J, Varewyck H. Joint report of the Third International Workshop on Lymphocyte Alloantigens of the Horse, Kennett Square, Pennsylvania, 25-27 April 1984.. Anim Genet 1986;17(4):363-73.
    pubmed: 3826760doi: 10.1111/j.1365-2052.1986.tb00730.xgoogle scholar: lookup
  7. Antczak DF, Bright SM, Remick LH, Bauman BE. Lymphocyte alloantigens of the horse. I. Serologic and genetic studies.. Tissue Antigens 1982 Sep;20(3):172-87.
    pubmed: 6182639doi: 10.1111/j.1399-0039.1982.tb00343.xgoogle scholar: lookup
  8. Assarsson E, Sidney J, Oseroff C, Pasquetto V, Bui HH, Frahm N, Brander C, Peters B, Grey H, Sette A. A quantitative analysis of the variables affecting the repertoire of T cell specificities recognized after vaccinia virus infection.. J Immunol 2007 Jun 15;178(12):7890-901.
    pubmed: 17548627doi: 10.4049/jimmunol.178.12.7890google scholar: lookup
  9. Azab W, Harman R, Miller D, Tallmadge R, Frampton AR, Antczak DF, Osterrieder N. Equid herpesvirus type 4 uses a restricted set of equine major histocompatibility complex class I proteins as entry receptors.. J Gen Virol 2014 Jul;95(Pt 7):1554-1563.
    pubmed: 24722677doi: 10.1099/vir.0.066407-0google scholar: lookup
  10. Bailey E, Marti E, Fraxer DG, Antczak DF, Lazary S. Immunogenetics of the horse. In: Bowling AT, Ruvinski T, editors. The genetics of the horse. Wallingford, UK: CABI; 2000.
  11. Bairoch A, Apweiler R. The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000.. Nucleic Acids Res 2000 Jan 1;28(1):45-8.
    pmc: PMC102476pubmed: 10592178doi: 10.1093/nar/28.1.45google scholar: lookup
  12. Cheng Y, Prusoff WH. Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction.. Biochem Pharmacol 1973 Dec 1;22(23):3099-108.
    pubmed: 4202581doi: 10.1016/0006-2952(73)90196-2google scholar: lookup
  13. Derbigny WA, Kim SK, Caughman GB, O'Callaghan DJ. The EICP22 protein of equine herpesvirus 1 physically interacts with the immediate-early protein and with itself to form dimers and higher-order complexes.. J Virol 2000 Feb;74(3):1425-35.
    pmc: PMC111477pubmed: 10627553doi: 10.1128/jvi.74.3.1425-1435.2000google scholar: lookup
  14. DOLL ER, CROWE ME, BRYANS JT, McCOLLUM WH. Infection immunity in equine virus abortion.. Cornell Vet 1955 Jul;45(3):387-410.
    pubmed: 14390961
  15. Earley L, Anderson LC, Bai DL, Mullen C, Syka JE, English AM, Dunyach JJ, Stafford GC Jr, Shabanowitz J, Hunt DF, Compton PD. Front-end electron transfer dissociation: a new ionization source.. Anal Chem 2013 Sep 3;85(17):8385-90.
    pmc: PMC3822909pubmed: 23909443doi: 10.1021/ac401783fgoogle scholar: lookup
  16. Gaddum RM, Ellis SA, Willis AC, Cook RS, Staines KA, Thomas LH, Taylor G. Identification of potential CTL epitopes of bovine RSV using allele-specific peptide motifs from bovine MHC class I molecules.. Vet Immunol Immunopathol 1996 Nov;54(1-4):211-9.
    pubmed: 8988867doi: 10.1016/s0165-2427(96)05686-3google scholar: lookup
  17. Geer LY, Markey SP, Kowalak JA, Wagner L, Xu M, Maynard DM, Yang X, Shi W, Bryant SH. Open mass spectrometry search algorithm.. J Proteome Res 2004 Sep-Oct;3(5):958-64.
    pubmed: 15473683doi: 10.1021/pr0499491google scholar: lookup
  18. Germain RN, Margulies DH. The biochemistry and cell biology of antigen processing and presentation.. Annu Rev Immunol 1993;11:403-50.
    pubmed: 8476568doi: 10.1146/annurev.iy.11.040193.002155google scholar: lookup
  19. Gillet L, May JS, Colaco S, Stevenson PG. The murine gammaherpesvirus-68 gp150 acts as an immunogenic decoy to limit virion neutralization.. PLoS One 2007 Aug 8;2(8):e705.
    pmc: PMC1931612pubmed: 17684552doi: 10.1371/journal.pone.0000705google scholar: lookup
  20. Gubler B, Daniel S, Armandola EA, Hammer J, Caillat-Zucman S, van Endert PM. Substrate selection by transporters associated with antigen processing occurs during peptide binding to TAP.. Mol Immunol 1998 Jun;35(8):427-33.
    pubmed: 9798647doi: 10.1016/s0161-5890(98)00059-5google scholar: lookup
  21. Gulukota K, Sidney J, Sette A, DeLisi C. Two complementary methods for predicting peptides binding major histocompatibility complex molecules.. J Mol Biol 1997 Apr 18;267(5):1258-67.
    pubmed: 9150410doi: 10.1006/jmbi.1997.0937google scholar: lookup
  22. Gustafson AL, Tallmadge RL, Ramlachan N, Miller D, Bird H, Antczak DF, Raudsepp T, Chowdhary BP, Skow LC. An ordered BAC contig map of the equine major histocompatibility complex.. Cytogenet Genome Res 2003;102(1-4):189-95.
    pubmed: 14970701doi: 10.1159/000075747google scholar: lookup
  23. Guzman E, Taylor G, Charleston B, Ellis SA. Induction of a cross-reactive CD8(+) T cell response following foot-and-mouth disease virus vaccination.. J Virol 2010 Dec;84(23):12375-84.
    pmc: PMC2976409pubmed: 20861264doi: 10.1128/jvi.01545-10google scholar: lookup
  24. Hagen J, Hartnett C, Houchins JP, Giguère S, Kalyuzhny AE. Equine ELISPOT assay to study secretion of IFNγ and IL-4 from peripheral blood mononuclear cells.. Methods Mol Biol 2012;792:39-45.
    pubmed: 21956499doi: 10.1007/978-1-61779-325-7_3google scholar: lookup
  25. Hannant D, Jessett DM, O'Neill T, Dolby CA, Cook RF, Mumford JA. Responses of ponies to equid herpesvirus-1 ISCOM vaccination and challenge with virus of the homologous strain.. Res Vet Sci 1993 May;54(3):299-305.
    pubmed: 8393207doi: 10.1016/0034-5288(93)90126-zgoogle scholar: lookup
  26. Heldens JG, Hannant D, Cullinane AA, Prendergast MJ, Mumford JA, Nelly M, Kydd JH, Weststrate MW, van den Hoven R. Clinical and virological evaluation of the efficacy of an inactivated EHV1 and EHV4 whole virus vaccine (Duvaxyn EHV1,4). Vaccination/challenge experiments in foals and pregnant mares.. Vaccine 2001 Jul 20;19(30):4307-17.
    pubmed: 11457558doi: 10.1016/s0264-410x(01)00131-1google scholar: lookup
  27. Huang T, Lehmann MJ, Said A, Ma G, Osterrieder N. Major histocompatibility complex class I downregulation induced by equine herpesvirus type 1 pUL56 is through dynamin-dependent endocytosis.. J Virol 2014 Nov;88(21):12802-15.
    pmc: PMC4248885pubmed: 25165105doi: 10.1128/jvi.02079-14google scholar: lookup
  28. Kim SK, Jang HK, Albrecht RA, Derbigny WA, Zhang Y, O'Callaghan DJ. Interaction of the equine herpesvirus 1 EICP0 protein with the immediate-early (IE) protein, TFIIB, and TBP may mediate the antagonism between the IE and EICP0 proteins.. J Virol 2003 Feb;77(4):2675-85.
    pmc: PMC141080pubmed: 12552007doi: 10.1128/jvi.77.4.2675-2685.2003google scholar: lookup
  29. Koppers-Lalic D, Verweij MC, Lipińska AD, Wang Y, Quinten E, Reits EA, Koch J, Loch S, Marcondes Rezende M, Daus F, Bieńkowska-Szewczyk K, Osterrieder N, Mettenleiter TC, Heemskerk MH, Tampé R, Neefjes JJ, Chowdhury SI, Ressing ME, Rijsewijk FA, Wiertz EJ. Varicellovirus UL 49.5 proteins differentially affect the function of the transporter associated with antigen processing, TAP.. PLoS Pathog 2008 May 30;4(5):e1000080.
    pmc: PMC2386557pubmed: 18516302doi: 10.1371/journal.ppat.1000080google scholar: lookup
  30. Kotturi MF, Peters B, Buendia-Laysa F Jr, Sidney J, Oseroff C, Botten J, Grey H, Buchmeier MJ, Sette A. The CD8+ T-cell response to lymphocytic choriomeningitis virus involves the L antigen: uncovering new tricks for an old virus.. J Virol 2007 May;81(10):4928-40.
    pmc: PMC1900207pubmed: 17329346doi: 10.1128/jvi.02632-06google scholar: lookup
  31. Kydd JH, Davis-Poynter NJ, Birch J, Hannant D, Minke J, Audonnet JC, Antczak DF, Ellis SA. A molecular approach to the identification of cytotoxic T-lymphocyte epitopes within equine herpesvirus 1.. J Gen Virol 2006 Sep;87(Pt 9):2507-2515.
    pubmed: 16894188doi: 10.1099/vir.0.82070-0google scholar: lookup
  32. Kydd JH, Wattrang E, Hannant D. Pre-infection frequencies of equine herpesvirus-1 specific, cytotoxic T lymphocytes correlate with protection against abortion following experimental infection of pregnant mares.. Vet Immunol Immunopathol 2003 Dec 15;96(3-4):207-17.
    pubmed: 14592733doi: 10.1016/j.vetimm.2003.08.004google scholar: lookup
  33. Lanier LL. Natural killer cell receptors and MHC class I interactions.. Curr Opin Immunol 1997 Feb;9(1):126-31.
    pubmed: 9039769doi: 10.1016/s0952-7915(97)80169-0google scholar: lookup
  34. Lauemøller SL, Holm A, Hilden J, Brunak S, Holst Nissen M, Stryhn A, Østergaard Pedersen L, Buus S. Quantitative predictions of peptide binding to MHC class I molecules using specificity matrices and anchor-stratified calibrations.. Tissue Antigens 2001 May;57(5):405-14.
    pubmed: 11556965doi: 10.1034/j.1399-0039.2001.057005405.xgoogle scholar: lookup
  35. Lazary S, Antczak DF, Bailey E, Bell TK, Bernoco D, Byrns G, McClure JJ. Joint Report of the Fifth International Workshop on Lymphocyte Alloantigens of the Horse, Baton Rouge, Louisiana, 31 October-1 November 1987.. Anim Genet 1988;19(4):447-56.
    pubmed: 2466424doi: 10.1111/j.1365-2052.1988.tb00836.xgoogle scholar: lookup
  36. Loffredo JT, Sidney J, Bean AT, Beal DR, Bardet W, Wahl A, Hawkins OE, Piaskowski S, Wilson NA, Hildebrand WH, Watkins DI, Sette A. Two MHC class I molecules associated with elite control of immunodeficiency virus replication, Mamu-B*08 and HLA-B*2705, bind peptides with sequence similarity.. J Immunol 2009 Jun 15;182(12):7763-75.
    pmc: PMC2701622pubmed: 19494300doi: 10.4049/jimmunol.0900111google scholar: lookup
  37. Lund O, Nielsen M, Kesmir C, Petersen AG, Lundegaard C, Worning P, Sylvester-Hvid C, Lamberth K, Røder G, Justesen S, Buus S, Brunak S. Definition of supertypes for HLA molecules using clustering of specificity matrices.. Immunogenetics 2004 Mar;55(12):797-810.
    pubmed: 14963618doi: 10.1007/s00251-004-0647-4google scholar: lookup
  38. Ma G, Azab W, Osterrieder N. Equine herpesviruses type 1 (EHV-1) and 4 (EHV-4)--masters of co-evolution and a constant threat to equids and beyond.. Vet Microbiol 2013 Nov 29;167(1-2):123-34.
    pubmed: 23890672doi: 10.1016/j.vetmic.2013.06.018google scholar: lookup
  39. McGuire TC, Leib SR, Mealey RH, Fraser DG, Prieur DJ. Presentation and binding affinity of equine infectious anemia virus CTL envelope and matrix protein epitopes by an expressed equine classical MHC class I molecule.. J Immunol 2003 Aug 15;171(4):1984-93.
    pubmed: 12902502doi: 10.4049/jimmunol.171.4.1984google scholar: lookup
  40. Mealey RH, Lee JH, Leib SR, Littke MH, McGuire TC. A single amino acid difference within the alpha-2 domain of two naturally occurring equine MHC class I molecules alters the recognition of Gag and Rev epitopes by equine infectious anemia virus-specific CTL.. J Immunol 2006 Nov 15;177(10):7377-90.
    pmc: PMC3342702pubmed: 17082657doi: 10.4049/jimmunol.177.10.7377google scholar: lookup
  41. Neefjes J, Jongsma ML, Paul P, Bakke O. Towards a systems understanding of MHC class I and MHC class II antigen presentation.. Nat Rev Immunol 2011 Nov 11;11(12):823-36.
    pubmed: 22076556doi: 10.1038/nri3084google scholar: lookup
  42. O'Neill T, Kydd JH, Allen GP, Wattrang E, Mumford JA, Hannant D. Determination of equid herpesvirus 1-specific, CD8+, cytotoxic T lymphocyte precursor frequencies in ponies.. Vet Immunol Immunopathol 1999 Sep 1;70(1-2):43-54.
    pubmed: 10507286doi: 10.1016/s0165-2427(99)00037-9google scholar: lookup
  43. Ostlund EN. The equine herpesviruses.. Vet Clin North Am Equine Pract 1993 Aug;9(2):283-94.
    pubmed: 8395324doi: 10.1016/s0749-0739(17)30396-6google scholar: lookup
  44. Paillot R, Ellis SA, Daly JM, Audonnet JC, Minke JM, Davis-Poynter N, Hannant D, Kydd JH. Characterisation of CTL and IFN-gamma synthesis in ponies following vaccination with a NYVAC-based construct coding for EHV-1 immediate early gene, followed by challenge infection.. Vaccine 2006 Mar 6;24(10):1490-500.
    pubmed: 16269205doi: 10.1016/j.vaccine.2005.10.019google scholar: lookup
  45. Patel JR, Heldens J. Equine herpesviruses 1 (EHV-1) and 4 (EHV-4)--epidemiology, disease and immunoprophylaxis: a brief review.. Vet J 2005 Jul;170(1):14-23.
    pubmed: 15993786doi: 10.1016/j.tvjl.2004.04.018google scholar: lookup
  46. Paul S, Weiskopf D, Angelo MA, Sidney J, Peters B, Sette A. HLA class I alleles are associated with peptide-binding repertoires of different size, affinity, and immunogenicity.. J Immunol 2013 Dec 15;191(12):5831-9.
    pmc: PMC3872965pubmed: 24190657doi: 10.4049/jimmunol.1302101google scholar: lookup
  47. Peters B, Bui HH, Frankild S, Nielson M, Lundegaard C, Kostem E, Basch D, Lamberth K, Harndahl M, Fleri W, Wilson SS, Sidney J, Lund O, Buus S, Sette A. A community resource benchmarking predictions of peptide binding to MHC-I molecules.. PLoS Comput Biol 2006 Jun 9;2(6):e65.
    pmc: PMC1475712pubmed: 16789818doi: 10.1371/journal.pcbi.0020065google scholar: lookup
  48. Pinilla C, Appel JR, Blanc P, Houghten RA. Rapid identification of high affinity peptide ligands using positional scanning synthetic peptide combinatorial libraries.. Biotechniques 1992 Dec;13(6):901-5.
    pubmed: 1476743
  49. Rappocciolo G, Birch J, Ellis SA. Down-regulation of MHC class I expression by equine herpesvirus-1.. J Gen Virol 2003 Feb;84(Pt 2):293-300.
    pubmed: 12560560doi: 10.1099/vir.0.18612-0google scholar: lookup
  50. Reed JS, Sidney J, Piaskowski SM, Glidden CE, León EJ, Burwitz BJ, Kolar HL, Eernisse CM, Furlott JR, Maness NJ, Walsh AD, Rudersdorf RA, Bardet W, McMurtrey CP, O'Connor DH, Hildebrand WH, Sette A, Watkins DI, Wilson NA. The role of MHC class I allele Mamu-A*07 during SIV(mac)239 infection.. Immunogenetics 2011 Dec;63(12):789-807.
    pmc: PMC3706270pubmed: 21732180doi: 10.1007/s00251-011-0541-9google scholar: lookup
  51. Rosas CT, Goodman LB, von Einem J, Osterrieder N. Equine herpesvirus type 1 modified live virus vaccines: quo vaditis?. Expert Rev Vaccines 2006 Feb;5(1):119-31.
    pubmed: 16451114doi: 10.1586/14760584.5.1.119google scholar: lookup
  52. Rötzschke O, Falk K. Naturally-occurring peptide antigens derived from the MHC class-I-restricted processing pathway.. Immunol Today 1991 Dec;12(12):447-55.
    pubmed: 1723878doi: 10.1016/0167-5699(91)90018-ogoogle scholar: lookup
  53. Ruppert J, Sidney J, Celis E, Kubo RT, Grey HM, Sette A. Prominent role of secondary anchor residues in peptide binding to HLA-A2.1 molecules.. Cell 1993 Sep 10;74(5):929-37.
    pubmed: 8104103doi: 10.1016/0092-8674(93)90472-3google scholar: lookup
  54. Saper MA, Bjorkman PJ, Wiley DC. Refined structure of the human histocompatibility antigen HLA-A2 at 2.6 A resolution.. J Mol Biol 1991 May 20;219(2):277-319.
    pubmed: 2038058doi: 10.1016/0022-2836(91)90567-pgoogle scholar: lookup
  55. Serwold T, Gaw S, Shastri N. ER aminopeptidases generate a unique pool of peptides for MHC class I molecules.. Nat Immunol 2001 Jul;2(7):644-51.
    pubmed: 11429550doi: 10.1038/89800google scholar: lookup
  56. Sette A, Vitiello A, Reherman B, Fowler P, Nayersina R, Kast WM, Melief CJ, Oseroff C, Yuan L, Ruppert J, Sidney J, del Guercio MF, Southwood S, Kubo RT, Chesnut RW, Grey HM, Chisari FV. The relationship between class I binding affinity and immunogenicity of potential cytotoxic T cell epitopes.. J Immunol 1994 Dec 15;153(12):5586-92.
    pubmed: 7527444
  57. Sidney J, Assarsson E, Moore C, Ngo S, Pinilla C, Sette A, Peters B. Quantitative peptide binding motifs for 19 human and mouse MHC class I molecules derived using positional scanning combinatorial peptide libraries.. Immunome Res 2008 Jan 25;4:2.
    pmc: PMC2248166pubmed: 18221540doi: 10.1186/1745-7580-4-2google scholar: lookup
  58. Sidney J, Peters B, Frahm N, Brander C, Sette A. HLA class I supertypes: a revised and updated classification.. BMC Immunol 2008 Jan 22;9:1.
    pmc: PMC2245908pubmed: 18211710doi: 10.1186/1471-2172-9-1google scholar: lookup
  59. Sidney J, Peters B, Moore C, Pencille TJ, Ngo S, Masterman KA, Asabe S, Pinilla C, Chisari FV, Sette A. Characterization of the peptide-binding specificity of the chimpanzee class I alleles A 0301 and A 0401 using a combinatorial peptide library.. Immunogenetics 2007 Sep;59(9):745-51.
    pubmed: 17701407doi: 10.1007/s00251-007-0243-5google scholar: lookup
  60. Sidney J, Southwood S, Moore C, Oseroff C, Pinilla C, Grey HM, Sette A. Measurement of MHC/peptide interactions by gel filtration or monoclonal antibody capture.. Curr Protoc Immunol 2013 Feb;Chapter 18:Unit 18.3..
    pmc: PMC3626435pubmed: 23392640doi: 10.1002/0471142735.im1803s100google scholar: lookup
  61. Slingluff CL Jr, Cox AL, Henderson RA, Hunt DF, Engelhard VH. Recognition of human melanoma cells by HLA-A2.1-restricted cytotoxic T lymphocytes is mediated by at least six shared peptide epitopes.. J Immunol 1993 Apr 1;150(7):2955-63.
    pubmed: 7681084
  62. Soboll G, Breathnach CC, Kydd JH, Hussey SB, Mealey RM, Lunn DP. Vaccination of ponies with the IE gene of EHV-1 in a recombinant modified live vaccinia vector protects against clinical and virological disease.. Vet Immunol Immunopathol 2010 May 15;135(1-2):108-117.
    pubmed: 20018383doi: 10.1016/j.vetimm.2009.11.009google scholar: lookup
  63. Soboll G, Whalley JM, Koen MT, Allen GP, Fraser DG, Macklin MD, Swain WF, Lunn DP. Identification of equine herpesvirus-1 antigens recognized by cytotoxic T lymphocytes.. J Gen Virol 2003 Oct;84(Pt 10):2625-2634.
    pubmed: 13679596doi: 10.1099/vir.0.19268-0google scholar: lookup
  64. Southwood S, Solomon C, Hoof I, Rudersdorf R, Sidney J, Peters B, Wahl A, Hawkins O, Hildebrand W, Mothé BR, Sette A. Functional analysis of frequently expressed Chinese rhesus macaque MHC class I molecules Mamu-A1*02601 and Mamu-B*08301 reveals HLA-A2 and HLA-A3 supertypic specificities.. Immunogenetics 2011 May;63(5):275-90.
    pmc: PMC3068250pubmed: 21274527doi: 10.1007/s00251-010-0502-8google scholar: lookup
  65. Spurgin LG, Richardson DS. How pathogens drive genetic diversity: MHC, mechanisms and misunderstandings.. Proc Biol Sci 2010 Apr 7;277(1684):979-88.
    pmc: PMC2842774pubmed: 20071384doi: 10.1098/rspb.2009.2084google scholar: lookup
  66. Stryhn A, Pedersen LO, Romme T, Holm CB, Holm A, Buus S. Peptide binding specificity of major histocompatibility complex class I resolved into an array of apparently independent subspecificities: quantitation by peptide libraries and improved prediction of binding.. Eur J Immunol 1996 Aug;26(8):1911-8.
    pubmed: 8765039doi: 10.1002/eji.1830260836google scholar: lookup
  67. Swets JA. Measuring the accuracy of diagnostic systems.. Science 1988 Jun 3;240(4857):1285-93.
    pubmed: 3287615doi: 10.1126/science.3287615google scholar: lookup
  68. Tallmadge RL, Campbell JA, Miller DC, Antczak DF. Analysis of MHC class I genes across horse MHC haplotypes.. Immunogenetics 2010 Mar;62(3):159-72.
    pmc: PMC2872545pubmed: 20099063doi: 10.1007/s00251-009-0420-9google scholar: lookup
  69. Tallmadge RL, Lear TL, Antczak DF. Genomic characterization of MHC class I genes of the horse.. Immunogenetics 2005 Nov;57(10):763-74.
    pubmed: 16220348doi: 10.1007/s00251-005-0034-9google scholar: lookup
  70. Trolle T, Metushi IG, Greenbaum JA, Kim Y, Sidney J, Lund O, Sette A, Peters B, Nielsen M. Automated benchmarking of peptide-MHC class I binding predictions.. Bioinformatics 2015 Jul 1;31(13):2174-81.
    pmc: PMC4481849pubmed: 25717196doi: 10.1093/bioinformatics/btv123google scholar: lookup
  71. Tseng CT, Miller D, Cassano J, Bailey E, Antczak DF. Identification of equine major histocompatibility complex haplotypes using polymorphic microsatellites.. Anim Genet 2010 Dec;41 Suppl 2(Suppl 2):150-3.
    pmc: PMC3289534pubmed: 21070289doi: 10.1111/j.1365-2052.2010.02125.xgoogle scholar: lookup
  72. Udaka K, Wiesmüller KH, Kienle S, Jung G, Tamamura H, Yamagishi H, Okumura K, Walden P, Suto T, Kawasaki T. An automated prediction of MHC class I-binding peptides based on positional scanning with peptide libraries.. Immunogenetics 2000 Aug;51(10):816-28.
    pubmed: 10970096doi: 10.1007/s002510000217google scholar: lookup
  73. Udaka K, Wiesmüller KH, Kienle S, Jung G, Walden P. Decrypting the structure of major histocompatibility complex class I-restricted cytotoxic T lymphocyte epitopes with complex peptide libraries.. J Exp Med 1995 Jun 1;181(6):2097-108.
    pmc: PMC2192062pubmed: 7539039doi: 10.1084/jem.181.6.2097google scholar: lookup
  74. Udeshi ND, Compton PD, Shabanowitz J, Hunt DF, Rose KL. Methods for analyzing peptides and proteins on a chromatographic timescale by electron-transfer dissociation mass spectrometry.. Nat Protoc 2008;3(11):1709-17.
    pmc: PMC2860270pubmed: 18927556doi: 10.1038/nprot.2008.159google scholar: lookup
  75. Vaiman M, Chardon P, Cohen D. DNA polymorphism in the major histocompatibility complex of man and various farm animals.. Anim Genet 1986;17(2):113-33.
    pubmed: 2426996doi: 10.1111/j.1365-2052.1986.tb00732.xgoogle scholar: lookup
  76. Van de Walle GR, Goupil R, Wishon C, Damiani A, Perkins GA, Osterrieder N. A single-nucleotide polymorphism in a herpesvirus DNA polymerase is sufficient to cause lethal neurological disease.. J Infect Dis 2009 Jul 1;200(1):20-5.
    pubmed: 19456260doi: 10.1086/599316google scholar: lookup
  77. Wang P, Sidney J, Dow C, Mothé B, Sette A, Peters B. A systematic assessment of MHC class II peptide binding predictions and evaluation of a consensus approach.. PLoS Comput Biol 2008 Apr 4;4(4):e1000048.
    pmc: PMC2267221pubmed: 18389056doi: 10.1371/journal.pcbi.1000048google scholar: lookup
  78. Weiskopf D, Yauch LE, Angelo MA, John DV, Greenbaum JA, Sidney J, Kolla RV, De Silva AD, de Silva AM, Grey H, Peters B, Shresta S, Sette A. Insights into HLA-restricted T cell responses in a novel mouse model of dengue virus infection point toward new implications for vaccine design.. J Immunol 2011 Oct 15;187(8):4268-79.
    pmc: PMC3186824pubmed: 21918184doi: 10.4049/jimmunol.1101970google scholar: lookup
  79. Zhang W, Lonning SM, McGuire TC. Gag protein epitopes recognized by ELA-A-restricted cytotoxic T lymphocytes from horses with long-term equine infectious anemia virus infection.. J Virol 1998 Dec;72(12):9612-20.
    pmc: PMC110470pubmed: 9811694doi: 10.1128/jvi.72.12.9612-9620.1998google scholar: lookup
  80. Zhao Y, Holden VR, Smith RH, O'Callaghan DJ. Regulatory function of the equine herpesvirus 1 ICP27 gene product.. J Virol 1995 May;69(5):2786-93.
    pmc: PMC188972pubmed: 7707500doi: 10.1128/jvi.69.5.2786-2793.1995google scholar: lookup

Citations

This article has been cited 4 times.
  1. Vasoya D, Tzelos T, Benedictus L, Karagianni AE, Pirie S, Marr C, Oddsdóttir C, Fintl C, Connelley T. High-Resolution Genotyping of Expressed Equine MHC Reveals a Highly Complex MHC Structure.. Genes (Basel) 2023 Jul 10;14(7).
    doi: 10.3390/genes14071422pubmed: 37510326google scholar: lookup
  2. Yan S, Huang B, Bai X, Zhou Y, Guo L, Wang T, Shan Y, Wang Y, Tan F, Tian K. Construction and Immunogenicity of a Recombinant Pseudorabies Virus Variant With TK/gI/gE/11k/28k Deletion.. Front Vet Sci 2021;8:797611.
    doi: 10.3389/fvets.2021.797611pubmed: 35146013google scholar: lookup
  3. Sturm T, Sautter B, Wörner TP, Stevanović S, Rammensee HG, Planz O, Heck AJR, Aebersold R. Mild Acid Elution and MHC Immunoaffinity Chromatography Reveal Similar Albeit Not Identical Profiles of the HLA Class I Immunopeptidome.. J Proteome Res 2021 Jan 1;20(1):289-304.
    doi: 10.1021/acs.jproteome.0c00386pubmed: 33141586google scholar: lookup
  4. Bergmann T, Lindvall M, Moore E, Moore E, Sidney J, Miller D, Tallmadge RL, Myers PT, Malaker SA, Shabanowitz J, Osterrieder N, Peters B, Hunt DF, Antczak DF, Sette A. Peptide-binding motifs of two common equine class I MHC molecules in Thoroughbred horses.. Immunogenetics 2017 May;69(5):351-358.
    doi: 10.1007/s00251-017-0978-6pubmed: 28315936google scholar: lookup
Subscribe to our newsletter
Join 99,357 horse owners like you who receive our email updates on horse health and nutrition.