The development and integrity of equine pre-antral follicles cultured in vitro with follicle-stimulating hormone (FSH) supplementation.
Abstract: This study investigated the effects of different concentrations of FSH (10, 50, 100 and 200 ng/ml) in supplemented MEM+ on the development of equine pre-antral follicles that were cultured in vitro for 2 or 6 days. The ovaries (n = 5) from mares in seasonal anoestrus were collected from a local abattoir. Ten ovarian tissue fragments of approximately 3 × 3 × 1 mm were obtained from each animal. The fragments were cultured in situ for 2 days (D2) or 6 days (D6) in MEM+ or MEM+ supplemented with FSH at four different concentrations, establishing the following 11 groups: control (D0); MEM + (D2); MEM + (D6); MEM + 10 ng/ml of FSH (D2); MEM + 10 ng/ml of FSH (D6); MEM + 50 ng/ml of FSH (D2); MEM + 50 ng/ml of FSH (D6); MEM + 100 ng/ml of FSH (D2); MEM + 100 ng/ml of FSH (D6); MEM + 200 ng/ml of FSH (D2); and MEM + 200 ng/ml of FSH (D6). Follicles were observed in only 9.65% (388 of 4,018) of the histological sections. Of the 861 follicles evaluated, 488 were in the primordial stage, and 373 were in various developmental stages; 59.7% were morphologically normal. Regarding the integrity of the pre-antral follicles, the groups with 100 ng/ml FSH of 2-days culture as well as 50, 100 and 200 ng/ml FSH of 6-days culture provided the best results. In conclusion, the in vitro culture of abattoir-derived equine ovarian fragments presented better morphological integrity when supplemented with FSH for 6 days, in comparison with the MEM culture group. However, no clear effects were observed with FSH regarding the promotion of activation from a primordial to a developing follicle.
© 2017 Blackwell Verlag GmbH.
Publication Date: 2017-06-04 PubMed ID: 28580717DOI: 10.1111/rda.12996Google Scholar: Lookup
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- Journal Article
Summary
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This research explores how different levels of Follicle-Stimulating Hormone (FSH) affect the development of pre-antral follicles in horse ovaries. The study found that culturing fragments of equine ovarian tissue with FSH for six days resulted in better morphological integrity, compared with the control group.
Objective of the Study
- The main objective of this study was to research the impact of different concentrations of Follicle-Stimulating Hormone (FSH) on the development of equine pre-antral follicles when cultured in vitro for either two or six days.
Method and Experiment Groups
- The study used ovaries that were sourced from mares in seasonal anoestrus, obtained from a local abattoir. From each animal, the researchers took ten ovarian tissue fragments of approximately 3 x 3 x 1 mm.
- The researchers set up eleven experiment groups for the fragments to be cultured in situ, with groups differing based on the concentration of FSH used and whether the fragments were cultured for two or six days. These FSH concentrations included 10, 50, 100 and 200 ng/ml.
- There was also a control group that was not supplemented with FSH.
Observations and Results
- From the histological sections, only 9.65% (388 out of 4,018) showed follicles. Of the 861 follicles evaluated, 488 were at the primordial stage, and 373 were in various stages of development. About 59.7% were morphologically normal.
- When considering the morphological integrity of the pre-antral follicles, the groups that were cultured with 100 ng/ml FSH for two days, and the groups cultured with 50, 100, and 200 ng/ml FSH for six days provided the best results.
Conclusions
- The researchers concluded that culturing equine ovarian fragments sourced from an abattoir with an FSH supplement for six days resulted in better morphological integrity as compared to the control group.
- However, no clear effect could be seen with FSH regarding promoting the activation from a primordial follicle to a developing follicle.
Cite This Article
APA
Max MC, Silva CB, González SM, Lindquist AG, Búfalo I, Gomes RG, Morotti F, Costa CB, Barreiros T, Lisboa LA, Seneda MM.
(2017).
The development and integrity of equine pre-antral follicles cultured in vitro with follicle-stimulating hormone (FSH) supplementation.
Reprod Domest Anim, 52(5), 899-904.
https://doi.org/10.1111/rda.12996 Publication
Researcher Affiliations
- Laboratory of Animal Reproduction, University of Londrina, Londrina, PR, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Londrina, PR, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Londrina, PR, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Londrina, PR, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Londrina, PR, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Londrina, PR, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Londrina, PR, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Londrina, PR, Brazil.
- Laboratory of Biotechnology of Animal Reproduction, UENP, Bandeirantes, PR, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Londrina, PR, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Londrina, PR, Brazil.
MeSH Terms
- Anestrus
- Animals
- Culture Media
- Female
- Follicle Stimulating Hormone / pharmacology
- Horses
- Ovarian Follicle / drug effects
- Ovarian Follicle / growth & development
- Tissue Culture Techniques / methods
- Tissue Culture Techniques / veterinary
Citations
This article has been cited 1 times.- Max MC, Bizarro-Silva C, Búfalo I, González SM, Lindquist AG, Gomes RG, Barreiros TRR, Lisboa LA, Morotti F, Seneda MM. In vitro culture supplementation of EGF for improving the survival of equine preantral follicles. In Vitro Cell Dev Biol Anim 2018 Dec;54(10):687-691.
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