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Home / Equine Research Bank / Can Vet J / The effect of a gelatin β-tricalcium phosphate sponge loade...
The Canadian veterinary journal = La revue veterinaire canadienne2013; 54(6); 573-580;

The effect of a gelatin β-tricalcium phosphate sponge loaded with mesenchymal stem cells (MSC), bone morphogenic protein-2, and platelet-rich plasma (PRP) on equine articular cartilage defect.

Abstract: We evaluated the curative efficacy of a gelatin β-tricalcium phosphate (β-TCP) sponge loaded with mesenchymal stem cells (MSC), bone morphogenic protein-2 (BMP-2), and platelet-rich plasma (PRP) by insertion into an experimentally induced osteochondral defect. A hole of 10 mm diameter and depth was drilled in the bilateral medial femoral condyles of 7 thoroughbred horses, and into each either a loaded sponge (treatment) or a saline-infused β-TCP sponge (control) was inserted. After 16 weeks, defects were examined by computed tomography, macroscopic analyses, and histological analyses. The median subchondral bone density and macroscopic subscores for joint healing were significantly higher in the treatment legs (P < 0.05). Although there was no significant difference in total histological scores between groups, hyaline cartilaginous tissue was observed across a wider area in the treatment group. Equine joint healing can be enhanced by inserting a BMP-2-, MSC-, and PRP-impregnated β-TCP sponge at the lesion site. L’effet d’une éponge de phosphate β-tricalcique de gélatine imbibée de cellules souches mésenchymateuses (CSM), d’une protéine-2 morphogénétique osseuse et d’un plasma riche en plaquettes (PRP) sur un défaut de cartilage articulaire équin. Nous avons évalué l’efficacité curative d’une éponge de phosphate β-tricalcique de gélatine (β-TCP) imbibée de cellules souches mésenchymateuses (CSM), d’une protéine-2 morphogénétique osseuse (P2MO) et d’un plasma riche en plaquettes (PRP) en l’insérant dans un défaut ostéo-cartilagineux induit par expérimentation. Un trou de 10 mm de diamètre et de profondeur a été percé dans les condyles fémoraux médiaux bilatéraux de 7 pur-sang et, chez chaque cheval, une éponge imbibée (traitement) ou une éponge β-TCP infusée d’une solution saline (témoin) a été insérée. Après 16 semaines, les défauts ont été examinés par tomographie par ordinateur, analyses macroscopiques et analyses histologiques. La densité osseuse sous-chondrale et les sous-notes médianes de la guérison des articulations étaient significativement supérieures dans les jambes traitées (P < 0,05). Même s’il n’y avait pas de différences significatives au niveau des notes histologiques totales entre les groupes, le tissu cartilagineux hyalin a été observé sur une région plus vaste dans le groupe de traitement. La guérison des articulations équines peut être améliorée en insérant une éponge β-TCP imbibée de P2MO, de CSM et de PRP sur le site de la lésion.(Traduit par Isabelle Vallières).
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Publication Date: 2013-10-25 PubMed ID: 24155448PubMed Central: PMC3659453
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  • Controlled Clinical Trial
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the effectiveness of using a particular biomedical sponge combined with mesenchymal stem cells, proteins, and platelet-rich plasma on healing cartilage defects in horses.

Methodology

  • The experiment involved creating an osteochondral (relating to bone and cartilage) defect in seven thoroughbred horses. These were experimentally induced and consisted of a hole with a diameter and depth of 10mm in the medial femoral condyles of the animals.
  • The researchers then used a gelatin β-tricalcium phosphate (β-TCP) sponge in two different ways. For the treatment group, the sponge was loaded with mesenchymal stem cells (MSC), important for making and repairing skeletal tissues, bone morphogenic protein-2 (a protein signals cells to form bone and cartilage), and platelet-rich plasma (PRP-a concentration of blood cells that aids healing). The control group instead received a β-TCP sponge soaked in saline.
  • After 16 weeks, the defects were then assessed using computed tomography (a type of imaging), macroscopic analysis (the visual examination), and histological analysis (study of the microscopic structure of tissues).

Results

  • The research revealed that the treatment using the sponge loaded with MSC, BMP-2, and PRP resulted in a higher median density of the subchondral bone, which is the layer of bone just beneath the cartilage, compared to the control group.
  • The findings indicated more successful joint healing in the treatment legs than those of the control.
  • Additionally, the researchers did not find a significant difference in the total histological scores between the two groups. However, they did observe a wider area of hyaline cartilaginous tissue, a type of cartilage found on joint surfaces, in the treatment group than the control.

Conclusion

  • Therefore, the research suggests that equine joint healing can be promoted by inserting a sponge loaded with bone proteins, mesenchymal stem cells, and platelet-rich plasma at the lesion site.
  • This finding may be of clinical significance in the field of equine medicine and surgery, potentially offering a new approach to the treatment of joint injuries in horses.

Cite This Article

APA
Tsuzuki N, Seo JP, Yamada K, Haneda S, Furuoka H, Tabata Y, Sasaki N. (2013). The effect of a gelatin β-tricalcium phosphate sponge loaded with mesenchymal stem cells (MSC), bone morphogenic protein-2, and platelet-rich plasma (PRP) on equine articular cartilage defect. Can Vet J, 54(6), 573-580.

Publication

The Canadian veterinary journal = La revue veterinaire canadienne
ISSN: 0008-5286
NlmUniqueID: 0004653
Country: Canada
Language: English
Volume: 54
Issue: 6
Pages: 573-580

Researcher Affiliations

Tsuzuki, Nao
  • Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-city, Hokkaido, 080-8555, Japan (Tsuzuki, Seo, Yamada, Haneda, Sasaki); Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-city, Hokkaido, 080-8555, Japan (Furuoka); and Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, Sakyo-ku, Kyoto, 606-8507, Japan (Tabata).
Seo, Jong-pil
    Yamada, Kazutaka
      Haneda, Shingo
        Furuoka, Hidefumi
          Tabata, Yasuhiko
            Sasaki, Naoki

              MeSH Terms

              • Animals
              • Biomechanical Phenomena
              • Bone Morphogenetic Protein 2 / pharmacology
              • Calcium Phosphates / chemistry
              • Cartilage Diseases / therapy
              • Cartilage Diseases / veterinary
              • Cartilage, Articular / injuries
              • Cartilage, Articular / pathology
              • Female
              • Gelatin Sponge, Absorbable / chemistry
              • Gelatin Sponge, Absorbable / therapeutic use
              • Horse Diseases / therapy
              • Horses
              • Male
              • Mesenchymal Stem Cell Transplantation / methods
              • Mesenchymal Stem Cell Transplantation / veterinary
              • Platelet-Rich Plasma
              • Tissue Engineering
              • Weight-Bearing

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              Citations

              This article has been cited 4 times.
              1. Kováč J, Priščáková P, Gbelcová H, Heydari A, Žiaran S. Bioadhesive and Injectable Hydrogels and Their Correlation with Mesenchymal Stem Cells Differentiation for Cartilage Repair: A Mini-Review. Polymers (Basel) 2023 Oct 26;15(21).
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