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Home / Equine Research Bank / Vet Med Sci / The effect of equine bone marrow-derived mesenchymal stem ce...
Veterinary medicine and science2021; 7(3); 626-633; doi: 10.1002/vms3.427

The effect of equine bone marrow-derived mesenchymal stem cells on the expression of apoptotic genes in neutrophils.

Abstract: Bone marrow mesenchymal stem cells (BM-MSCs), as multipotent cells with self-renewal and plastic-adherent properties, have immunomodulatory effects on immune cells, including neutrophils. These cells are in close proximity in bone marrow (BM) sinusoids with non-multiplicative immature neutrophils. BM-MSCs exert their immunomodulatory effects on adjacent cells both directly (cell-to-cell contact) and indirectly (secretion of soluble factors). The aim of this study was to evaluate the effect of equine bone marrow mesenchymal stem cells (BM-MSCs) on the expression of some pro- and anti-apoptotic genes (p53, survivin and Bcl ) in neutrophils co-cultured with BM-MSCs. For this purpose, peripheral blood neutrophils were isolated and separately co-cultured for 12 hr with both BM-MSCs and the BM-MSCs΄ supernatant. Four groups were included: neutrophils with only culture media (as control), neutrophils co-cultured with BM-MScs, neutrophils cultured with BM-MSCs' supernatant and neutrophils cultured with lipopolysaccharide (LPS, as positive control). Then, the expression of mentioned genes (p53, survivin and Bcl ) was evaluated by quantitative polymerase chain reaction (qPCR). Compared with control neutrophils, in neutrophils co-cultured with both BM-MSCs and BM-MSCs' supernatant, the mRNA expression levels of p53, as pro-apoptotic gene, and survivin and Bcl , as anti-apoptotic genes, were remarkably increased and decreased (p < .05), respectively. These data revealed the notion that the direct contact of BM-MSCs is not obligatory for their effects on the apoptotic status of neutrophils and they affect neutrophils via soluble secreted factors, which is promising for clinical implications in equine medicine.
© 2021 The Authors Veterinary Medicine and Science Published by John Wiley & Sons Ltd.
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Publication Date: 2021-01-20 PubMed ID: 33471967PubMed Central: PMC8136922DOI: 10.1002/vms3.427Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study looked into how equine bone marrow mesenchymal stem cells (BM-MSCs) affect the expression of certain apoptosis-related genes in neutrophils. It specifically studied the potency of the stem cells both through direct contact and via secreted soluble factors, providing insights valuable for equine medicine.

Research Background and Objective

  • The study focuses on bone marrow mesenchymal stem cells (BM-MSCs), specifically from horses (equine). These stem cells are multipotent, meaning they can develop into different kinds of cells, and have the capacity to renew themselves.
  • BM-MSCs are known for their immunomodulatory effects on immune cells, particularly neutrophils. Neutrophils are a type of white blood cell that play a vital role in the body’s immune response.
  • The aim was to determine how the equine BM-MSCs impact the expression of some apoptosis-related genes in neutrophils. Apoptosis is the natural, programmed death of cells, and its regulation is important for maintaining health.

Experimental Procedures

  • To study this, the researchers isolated peripheral blood neutrophils and co-cultured them either directly with BM-MSCs or with the soluble factors released by these BM-MSCs (their supernatant).
  • There were four experimental groups: neutrophils with only culture media (as a control), neutrophils co-cultured with BM-MSCs, neutrophils cultured with the BM-MSCs’ supernatant, and neutrophils cultured with lipopolysaccharide (LPS, a substance used as a positive control).
  • They then measured the levels of mRNAs for a pro-apoptotic gene (p53) and two anti-apoptotic genes (survivin and Bcl) using quantitative polymerase chain reaction (qPCR).

Research Findings

  • The researchers discovered that, compared with the control neutrophils, those co-cultured with both the BM-MSCs and the BM-MSCs’ supernatant showed significant changes in gene expression.
  • The levels of mRNA for the p53 gene, which promotes apoptosis, were markedly increased. In contrast, the levels of mRNA for the survivin and Bcl genes, which inhibit apoptosis, were notably decreased.
  • These results suggest that direct contact with the BM-MSCs is not necessary for their effects on apoptosis to be manifested in neutrophils. Instead, the soluble secreted factors from these stem cells are enough to exert an effect.

Conclusion

  • This study concludes that BM-MSCs significantly affect apoptosis in neutrophils, not just through direct cell-to-cell contact but also via soluble factors they secreted.
  • This observation can have important implications for equine medicine, particularly in further understanding the immunomodulatory effects of stem cells and how they could be harnessed for health benefits.

Cite This Article

APA
Salami F, Ghodrati M, Parham A, Mehrzad J. (2021). The effect of equine bone marrow-derived mesenchymal stem cells on the expression of apoptotic genes in neutrophils. Vet Med Sci, 7(3), 626-633. https://doi.org/10.1002/vms3.427

Publication

Veterinary medicine and science
ISSN: 2053-1095
NlmUniqueID: 101678837
Country: England
Language: English
Volume: 7
Issue: 3
Pages: 626-633

Researcher Affiliations

Salami, Fatemeh
  • Division of Physiology, Department of Basic Sciences, Veterinary Faculty, Ferdowsi University of Mashhad, Mashhad, Iran.
Ghodrati, Mitra
  • Division of Physiology, Department of Basic Sciences, Veterinary Faculty, Ferdowsi University of Mashhad, Mashhad, Iran.
Parham, Abbas
  • Division of Physiology, Department of Basic Sciences, Veterinary Faculty, Ferdowsi University of Mashhad, Mashhad, Iran.
  • Stem Cell Biology and Regenerative Medicine Research Group, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
Mehrzad, Jalil
  • Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

MeSH Terms

  • Animals
  • Apoptosis / genetics
  • Bone Marrow
  • Female
  • Gene Expression Regulation, Developmental
  • Horses
  • Mesenchymal Stem Cells / metabolism
  • Neutrophils / metabolism

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Citations

This article has been cited 1 times.
  1. Abatay-Sel F, Erol A, Suleymanoglu M, Demirayak G, Kekik-Cinar C, Kuruca DS, Savran-Oguz F. The in vitro treatment of mesenchymal stem cells for colorectal cancer cells.. Med Oncol 2023 Feb 22;40(3):103.
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