The effect of growth hormone (GH) and insulin-like growth factor-I (IGF-I) on in vitro maturation of equine oocytes.
Abstract: The objective of this study was to test the hypothesis that equine growth hormone (eGH), in combination with insulin growth factor-I (IGF-I), influences positively in vitro nuclear and cytoplasmic maturation of equine oocytes. Cumulus-oocyte complexes were recovered from follicles that were < 25 mm in diameter, characterized by morphology and were allocated randomly as follow: (a) control (no additives); (b) 400 ng/ml eGH; (c) 200 ng/ml IGF-I; (d) eGH + IGF-I; and (e) eGH + IGF-I + 400 ng/ml anti-IGF-I antibody. Oocytes were matured for 30 h at 38.5°C in air with 5% CO2 and then stained with 10 μg/ml propidium iodide (PI) to evaluate nuclear status and 10 μg/ml Lens culinaris agglutinin-fluorescein complex (FITC-LCA) to assess cortical granule migration by confocal microscopy. The proportion of immature oocytes that developed to the metaphase II (MII) stage in the eGH + IGF-I group (15 of 45) was greater than in the groups that were treated only with IGF-I (7 of 36, p = 0.03). Oocytes that reached MII in the control group (20 of 56; 35.7%) showed a tendency to be different when compared with eGH + IGF-I group (15 of 45; 33.3%, p = 0.08). The treated group that contained anti-IGF-I (15 of 33; 45.4%) decreased the number of oocytes reaching any stage of development when compared with eGH (47 of 72; 65.3%) and eGH + IGF-I (33 of 45; 73.3%) groups (p = 0.05) when data from MI and MII were combined. We concluded that the addition of eGH to in vitro maturation (IVM) medium influenced the in vitro nuclear and cytoplasmic maturation of equine oocytes. The use of GH and IGF-I in vitro may represent a potential alternative for IVM of equine oocytes.
Publication Date: 2011-07-28 PubMed ID: 21794202DOI: 10.1017/S0967199411000335Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research study tested the joint influence of equine growth hormone and insulin growth factor-1 on the in vitro (laboratory-based) maturation process of horse oocytes, which are cells in an ovary involved in reproduction. The research concluded that the addition of these hormones could potentially improve the maturation process of the oocytes.
Research Setup
- The researchers collected cumulus-oocyte complexes from follicles that were smaller than 25 mm in diameter. The follicles’ morphology was assessed before they were randomly assigned to one of several groups, each treated differently:
- A control group, without added hormones.
- A group treated with 400 ng/ml of equine growth hormone (eGH).
- A group treated with 200 ng/ml of insulin growth factor-I (IGF-I).
- A group treated with a combination of eGH and IGF-I.
- A group treated with eGH, IGF-I, and an anti-IGF-I antibody.
- The oocytes were matured for 30 hours in a controlled atmosphere, then stained with propidium iodide (PI) and Lens culinaris agglutinin-fluorescein complex (FITC-LCA) to examine their nuclear status and cortical granule migration, respectively, under a confocal microscope.
Research Findings
- The study found that a higher proportion of immature oocytes developed to the Metaphase II (MII) stage in the group treated with both eGH and IGF-I than in the group treated solely with IGF-I.
- There was a marginal difference between the control group and the eGH + IGF-I group in the number of oocytes reaching MII, suggesting the hormones’ potential influence on this process.
- The group treated with anti-IGF-I showed a decreased number of oocytes reaching any stage of development than both the eGH only group and the eGH + IGF-I group, signifying the positive influence of IGF-I on oocyte development.
Conclusion
- Based on their observations, the researchers concluded that the addition of equine growth hormone to the in vitro maturation medium positively affects the in vitro maturation of equine oocytes, both at the nuclear and cytoplasmic level.
- The usage of growth hormone and insulin-like growth factor-I in vitro offers a promising alternative method for the in vitro maturation of equine oocytes.
Cite This Article
APA
Pereira GR, Lorenzo PL, Carneiro GF, Ball BA, Gonçalves PB, Pegoraro LM, Bilodeau-Goeseels S, Kastelic JP, Casey PJ, Liu IK.
(2011).
The effect of growth hormone (GH) and insulin-like growth factor-I (IGF-I) on in vitro maturation of equine oocytes.
Zygote, 20(4), 353-360.
https://doi.org/10.1017/S0967199411000335 Publication
Researcher Affiliations
- Laboratory of Biotechnology and Animal Reproduction-BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil. gabrielrp@biorep.ufsm.br
MeSH Terms
- Animals
- Cell Nucleus / drug effects
- Female
- Growth Hormone / pharmacology
- Horses
- Insulin-Like Growth Factor I / pharmacology
- Microscopy, Confocal
- Oocytes / cytology
- Oocytes / drug effects
- Oocytes / metabolism
- Ovarian Follicle / cytology
- Ovarian Follicle / drug effects
- Ovarian Follicle / metabolism
Citations
This article has been cited 6 times.- Deng K, Du D, Fan D, Pei Z, Zhang S, Xu C. Growth Hormone Promotes Oocyte Maturation In Vitro by Protecting Mitochondrial Function and Reducing Apoptosis.. Reprod Sci 2023 Jul;30(7):2219-2230.
- Chang CW, Sung YW, Hsueh YW, Chen YY, Ho M, Hsu HC, Yang TC, Lin WC, Chang HM. Growth hormone in fertility and infertility: Mechanisms of action and clinical applications.. Front Endocrinol (Lausanne) 2022;13:1040503.
- Pan P, Huang X. The Clinical Application of Growth Hormone and Its Biological and Molecular Mechanisms in Assisted Reproduction.. Int J Mol Sci 2022 Sep 15;23(18).
- Liu X, Hao Y, Li Z, Zhou J, Zhu H, Bu G, Liu Z, Hou X, Zhang X, Miao YL. Maternal Cytokines CXCL12, VEGFA, and WNT5A Promote Porcine Oocyte Maturation via MAPK Activation and Canonical WNT Inhibition.. Front Cell Dev Biol 2020;8:578.
- Lan KC, Lin PY, Chang YC, Chen YJ, Tsai YR, Ismaeil Mohamed IS, Kang HY. Growth hormone supplementation may improve the pregnancy rate and endometrial receptivity among women aged more than 40 years undergoing in vitro fertilization.. Biomed J 2019 Dec;42(6):411-416.
- Hull KL, Harvey S. Growth hormone and reproduction: a review of endocrine and autocrine/paracrine interactions.. Int J Endocrinol 2014;2014:234014.
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