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Equine veterinary journal2011; 44(5); 587-593; doi: 10.1111/j.2042-3306.2011.00514.x

The effect of intralesional injection of bone marrow derived mesenchymal stem cells and bone marrow supernatant on collagen fibril size in a surgical model of equine superficial digital flexor tendonitis.

Abstract: Collagen fibril size is decreased in repair tissue following tendon injury compared to normal tendon matrix in horses. Mesenchymal stem cells have been suggested to promote regeneration of tendon matrix rather than fibrotic repair following injury, although this concept remains unproven. Objective: To explore the hypothesis that implantation of autologous mesenchymal stem cells derived from bone marrow into a surgically created central core defect in the superficial digital flexor tendon (SDFT) of horses would induce the formation of a matrix with greater ultrastructural similarities to tendon matrix than the fibrotic scar tissue formed in control defects. Methods: Tissue was collected 16 weeks after induction of injury and 12 weeks after treatment from normal and injured regions of control and treated limbs of 6 horses and examined using transmission electron microscopy. Collagen fibril diameters were measured manually with image analysis software and surface areas calculated. Three parameters assessed for normal and injured tissue were mass average diameter (MAD), collagen fibril index (CFI) and the area dependent diameter (ADD). Results: Normal regions from both treated and control limbs displayed higher MAD and CFI values, as well as a characteristic bimodal distribution in fibril size. Injured regions from both treated and control limbs displayed significantly lower MAD and CFI values, as well as a unimodal distribution in fibril size. There were no significant differences between treated and control limbs for any of the parameters assessed. Conclusions: Intralesional injection of autologous bone marrow derived mesenchymal stem cells had no measurable effect on the fibril diameter of collagen in healing tissue in the SDFT of this experimental model 16 weeks after injury. Conclusions: Favouring matrix regeneration over fibrotic repair may not be the mechanism by which autologous mesenchymal stem cells assist healing of tendon injury.
© 2011 EVJ Ltd.
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Publication Date: 2011-12-11 PubMed ID: 22150794DOI: 10.1111/j.2042-3306.2011.00514.xGoogle Scholar: Lookup
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  • Clinical Trial
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aims to investigate if injecting stem cells derived from bone marrow into equine tendon injuries can lead to better healing by replicating the structural properties of a healthy tendon. However, the study found there was no significant change in the collagen fibril size in the injured area, suggesting that stem cells may not promote this kind of healing in tendon injuries.

Research Overview

  • Healing tendon injuries in horses is often associated with the formation of fibrotic scar tissue, which has less structural integrity compared to normal tendon tissue. Researchers have been considering the use of mesenchymal stem cells (MSCs), specifically those derived from the bone marrow, as an approach to resolve this issue.
  • The objective of this study was to investigate if such stem cells could stimulate a better form of healing in equine tendon injuries. The ‘better healing’ is defined as the creation of a matrix that more closely resembles that of a normal tendon rather than the fibrotic scarring observed in conventional healing.

Methodology

  • A surgical injury was created in the superficial digital flexor tendon (SDFT) of horses, followed by treatment using MSCs derived from bone marrow.
  • Sixteen weeks after the injury and twelve weeks after the treatment, tissue samples were collected from both normal and injured regions of the horses.
  • Transmission electron microscopy was used to examine the collagen fibril diameter within these tissue samples.
  • Parameters including mass average diameter (MAD), collagen fibril index (CFI), and the area-dependent diameter (ADD) were assessed.

Results

  • The study found that while normal regions showed higher MAD and CFI (indicating larger collagen fibrils), the injured regions showed significantly smaller values, suggesting the formation of scar tissue.
  • This result was uniform between both the treated (received stem cell injections) and control (no treatment) groups, indicating no significant effect of the stem cell treatment on collagen fibril size.

Conclusions

  • The study concludes that injecting autologous bone marrow-derived mesenchymal stem cells into equine tendon injuries does not promote the formation of larger collagen fibrils, which would more closely resemble a healthy tendon matrix.
  • Instead, it could hint that the stem cells might operate through an alternative healing mechanism while aiding in tendon injuries, if at all.

Cite This Article

APA
Caniglia CJ, Schramme MC, Smith RK. (2011). The effect of intralesional injection of bone marrow derived mesenchymal stem cells and bone marrow supernatant on collagen fibril size in a surgical model of equine superficial digital flexor tendonitis. Equine Vet J, 44(5), 587-593. https://doi.org/10.1111/j.2042-3306.2011.00514.x

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 44
Issue: 5
Pages: 587-593

Researcher Affiliations

Caniglia, C J
  • North Carolina State University, College of Veterinary Medicine, NC, USA.
Schramme, M C
    Smith, R K

      MeSH Terms

      • Animals
      • Bone Marrow Cells / physiology
      • Collagen / chemistry
      • Collagen / metabolism
      • Forelimb
      • Horse Diseases / etiology
      • Horse Diseases / therapy
      • Horses
      • Mesenchymal Stem Cell Transplantation / veterinary
      • Tendon Injuries / therapy
      • Tendon Injuries / veterinary
      • Wound Healing

      Citations

      This article has been cited 22 times.
      1. Roberts JH, Halper J. Growth Factor Roles in Soft Tissue Physiology and Pathophysiology. Adv Exp Med Biol 2021;1348:139-159.
        doi: 10.1007/978-3-030-80614-9_6pubmed: 34807418google scholar: lookup
      2. Gaesser AM, Underwood C, Linardi RL, Even KM, Reef VB, Shetye SS, Mauck RL, King WJ, Engiles JB, Ortved KF. Evaluation of Autologous Protein Solution Injection for Treatment of Superficial Digital Flexor Tendonitis in an Equine Model. Front Vet Sci 2021;8:697551.
        doi: 10.3389/fvets.2021.697551pubmed: 34291103google scholar: lookup
      3. Harman RM, Marx C, Van de Walle GR. Translational Animal Models Provide Insight Into Mesenchymal Stromal Cell (MSC) Secretome Therapy. Front Cell Dev Biol 2021;9:654885.
        doi: 10.3389/fcell.2021.654885pubmed: 33869217google scholar: lookup
      4. Chung MJ, Son JY, Park S, Park SS, Hur K, Lee SH, Lee EJ, Park JK, Hong IH, Kim TH, Jeong KS. Mesenchymal Stem Cell and MicroRNA Therapy of Musculoskeletal Diseases. Int J Stem Cells 2021 May 30;14(2):150-167.
        doi: 10.15283/ijsc20167pubmed: 33377459google scholar: lookup
      5. Ribitsch I, Baptista PM, Lange-Consiglio A, Melotti L, Patruno M, Jenner F, Schnabl-Feichter E, Dutton LC, Connolly DJ, van Steenbeek FG, Dudhia J, Penning LC. Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do. Front Bioeng Biotechnol 2020;8:972.
        doi: 10.3389/fbioe.2020.00972pubmed: 32903631google scholar: lookup
      6. Han Y, Li X, Zhang Y, Han Y, Chang F, Ding J. Mesenchymal Stem Cells for Regenerative Medicine. Cells 2019 Aug 13;8(8).
        doi: 10.3390/cells8080886pubmed: 31412678google scholar: lookup
      7. Shojaee A, Parham A. Strategies of tenogenic differentiation of equine stem cells for tendon repair: current status and challenges. Stem Cell Res Ther 2019 Jun 18;10(1):181.
        doi: 10.1186/s13287-019-1291-0pubmed: 31215490google scholar: lookup
      8. Tsang AS, Dart AJ, Biasutti SA, Jeffcott LB, Smith MM, Little CB. Effects of tendon injury on uninjured regional tendons in the distal limb: An in-vivo study using an ovine tendinopathy model. PLoS One 2019;14(4):e0215830.
        doi: 10.1371/journal.pone.0215830pubmed: 31013317google scholar: lookup
      9. Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H, Canciello A, Greco L, Muttini A, Gatta V, Stuppia L, Mattioli M. Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance. Cell Transplant 2018 Jan;27(1):93-116.
        doi: 10.1177/0963689717724797pubmed: 29562773google scholar: lookup
      10. Jacquet-Guibon S, Dupays AG, Coudry V, Crevier-Denoix N, Leroy S, Siñeriz F, Chiappini F, Barritault D, Denoix JM. Randomized controlled trial demonstrates the benefit of RGTA® based matrix therapy to treat tendinopathies in racing horses. PLoS One 2018;13(3):e0191796.
        doi: 10.1371/journal.pone.0191796pubmed: 29522564google scholar: lookup
      11. Geburek F, Roggel F, van Schie HTM, Beineke A, Estrada R, Weber K, Hellige M, Rohn K, Jagodzinski M, Welke B, Hurschler C, Conrad S, Skutella T, van de Lest C, van Weeren R, Stadler PM. Effect of single intralesional treatment of surgically induced equine superficial digital flexor tendon core lesions with adipose-derived mesenchymal stromal cells: a controlled experimental trial. Stem Cell Res Ther 2017 Jun 5;8(1):129.
        doi: 10.1186/s13287-017-0564-8pubmed: 28583184google scholar: lookup
      12. Jiang T, Xu G, Wang Q, Yang L, Zheng L, Zhao J, Zhang X. In vitro expansion impaired the stemness of early passage mesenchymal stem cells for treatment of cartilage defects. Cell Death Dis 2017 Jun 1;8(6):e2851.
        doi: 10.1038/cddis.2017.215pubmed: 28569773google scholar: lookup
      13. Lang HM, Schnabel LV, Cassano JM, Fortier LA. Effect of needle diameter on the viability of equine bone marrow derived mesenchymal stem cells. Vet Surg 2017 Jul;46(5):731-737.
        doi: 10.1111/vsu.12639pubmed: 28328147google scholar: lookup
      14. Berglund AK, Schnabel LV. Allogeneic major histocompatibility complex-mismatched equine bone marrow-derived mesenchymal stem cells are targeted for death by cytotoxic anti-major histocompatibility complex antibodies. Equine Vet J 2017 Jul;49(4):539-544.
        doi: 10.1111/evj.12647pubmed: 27862236google scholar: lookup
      15. Williams LB, Co C, Koenig JB, Tse C, Lindsay E, Koch TG. Response to Intravenous Allogeneic Equine Cord Blood-Derived Mesenchymal Stromal Cells Administered from Chilled or Frozen State in Serum and Protein-Free Media. Front Vet Sci 2016;3:56.
        doi: 10.3389/fvets.2016.00056pubmed: 27500136google scholar: lookup
      16. Williams LB, Russell KA, Koenig JB, Koch TG. Aspiration, but not injection, decreases cultured equine mesenchymal stromal cell viability. BMC Vet Res 2016 Mar 7;12:45.
        doi: 10.1186/s12917-016-0671-2pubmed: 26952099google scholar: lookup
      17. Behfar M, Javanmardi S, Sarrafzadeh-Rezaei F. Comparative study on functional effects of allotransplantation of bone marrow stromal cells and adipose derived stromal vascular fraction on tendon repair: a biomechanical study in rabbits. Cell J 2014 Fall;16(3):263-70.
        pubmed: 24611149
      18. Smith RK, Werling NJ, Dakin SG, Alam R, Goodship AE, Dudhia J. Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy. PLoS One 2013;8(9):e75697.
        doi: 10.1371/journal.pone.0075697pubmed: 24086616google scholar: lookup
      19. Carvalho Ade M, Badial PR, Álvarez LE, Yamada AL, Borges AS, Deffune E, Hussni CA, Garcia Alves AL. Equine tendonitis therapy using mesenchymal stem cells and platelet concentrates: a randomized controlled trial. Stem Cell Res Ther 2013 Jul 22;4(4):85.
        doi: 10.1186/scrt236pubmed: 23876512google scholar: lookup
      20. Muttini A, Salini V, Valbonetti L, Abate M. Stem cell therapy of tendinopathies: suggestions from veterinary medicine. Muscles Ligaments Tendons J 2012 Jul;2(3):187-92.
        pubmed: 23738296
      21. Gadallah S, Sharshar A, Fadel M, Mahran E, Hammad A. Ultrasonographic characterization of tendons and ligaments of palmar/plantar aspect of the cannon region in Egyptian donkeys. Iran J Vet Res 2024;25(2):143-155.
        doi: 10.22099/IJVR.2024.47480.6859pubmed: 39624193google scholar: lookup
      22. Tamura N, Heidari N, Faragher RGA, Smith RKW, Dudhia J. Effects of resveratrol and its analogues on the cell cycle of equine mesenchymal stem/stromal cells. J Equine Sci 2023 Sep;34(3):67-72.
        doi: 10.1294/jes.34.67pubmed: 37781569google scholar: lookup
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