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Home / Equine Research Bank / BMC Vet Res / The effect of lysophosphatidic acid on myometrial contractil...
BMC veterinary research2024; 20(1); 571; doi: 10.1186/s12917-024-04384-2

The effect of lysophosphatidic acid on myometrial contractility and the mRNA transcription of its receptors in the myometrium at different stages of endometrosis in mares.

Abstract: Endometrosis (chronic degenerative endometritis) results in morphological changes in the equine endometrium and impairs its secretory function. However, the effect of this condition on the myometrium remains unclear. Lysophosphatidic acid (LPA) may affect female reproductive function and embryo transport by influencing uterine contractility through its receptors (LPARs). The objective of this study was to determine myometrial LPAR1-6 mRNA transcription, and the effects of LPA on myometrial contractions in mares with endometrosis during the mid-luteal and follicular phases of the estrous cycle. Results: A reduction in myometrial LPAR1 mRNA transcription was observed in mares with endometrosis during the mid-luteal phase, in comparison to those with category I endometria (P < 0.05). While, upregulation of myometrial LPAR3 or LPAR6 mRNA transcription was observed in mares with category III or IIB endometria; respectively (P < 0.05). An increase in myometrial LPAR1, LPAR3 and LPAR5 mRNA transcription was observed during the follicular phase in mares with category IIA endometrium in comparison to their expression in category I endometrium (P < 0.05). During endometrosis progression LPA reduced the force of myometrial contractions in both phases of the estrous cycle (P < 0.05). However, in mares with category IIA endometrium during the follicular phase, LPA was found to increase the force of contraction of myometrial strips in comparison to mares with category I endometrium (P < 0.01). Conclusions: In the course of endometrosis in mares, a disruption in the myometrial mRNA transcription of LPARs has been observed. This is the first study to examine the impact of LPA on myometrial contractility at diffrent stage of endometrosis. However, it is essential to consider that multiple factors may contribute to this process. Alternations in contractile activity and changes in myometrial LPARs mRNA transcription may indicate impaired LPA-signaling mechanisms in equine myometrium during endometrosis.
© 2024. The Author(s).
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Publication Date: 2024-12-19 PubMed ID: 39696406PubMed Central: PMC11658124DOI: 10.1186/s12917-024-04384-2Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates how lysophosphatidic acid (LPA) affects the contraction of the myometrium muscle (which forms the uterine wall) and mRNA transcription of its receptors during different stages of endometrosis in mares. Endometrosis is a condition causing changes to the equine endometrium that may affect reproductive function.

Objective of the Study

  • The main goal of this study is to map the expression of myometrial LPAR1-6 mRNA, and the effects of LPA on myometrial contractions in mares suffering from endometrosis during the mid-luteal and follicular phases of the estrous cycle. The researchers sought to understand if and how LPA and its receptors (LPARs) influence uterine contractility in mares afflicted by endometrosis.

Findings of the Study

  • Different degrees of transcription changes were observed in myometrial LPAR1, LPAR3, LPAR5, and LPAR6 mRNA during different stages of endometrosis. In the mid-luteal phase, myometrial LPAR1 mRNA transcription was reduced in mares with endometrosis. An upregulation of myometrial LPAR3 or LPAR6 mRNA was observed in mares with category III or IIB endometria. An increase in myometrial LPAR1, LPAR3, and LPAR5 mRNA transcription was observed during the follicular phase in mares with category IIA endometrium.
  • The LPA was found to have varied effects on myometrial contractility depending on the stage of endometrosis. In general, LPA reduced the force of myometrial contractions in both phases of the estrous cycle. However, in mares with category IIA endometrium (lesions between glands) during the follicular phase, LPA was found to increase the force of contraction of myometrial strips.

Conclusion

  • The researchers concluded that endometrosis in mares leads to disrupted myometrial mRNA transcription of LPARs. This understanding is significant because it indicates that LPA-signaling mechanisms in equine myometrium might be impaired during endometrosis, which could have broader implications on the female reproductive function and embryo transport.
  • Additionally, the study suggests that multiple factors may contribute to myometrial contractility during endometrosis, and further research is required to fully elucidate this complex process.

Cite This Article

APA
Piotrowska-Tomala KK, Szóstek-Mioduchowska A, Jonczyk AW, Drzewiecka EM, Wrobel MH, Hojo T, Ferreira-Dias G, Skarzynski DJ. (2024). The effect of lysophosphatidic acid on myometrial contractility and the mRNA transcription of its receptors in the myometrium at different stages of endometrosis in mares. BMC Vet Res, 20(1), 571. https://doi.org/10.1186/s12917-024-04384-2

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 20
Issue: 1
Pages: 571
PII: 571

Researcher Affiliations

Piotrowska-Tomala, Katarzyna Karolina
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland.
Szóstek-Mioduchowska, Anna
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland.
Jonczyk, Agnieszka Walentyna
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland.
Drzewiecka, Ewa Monika
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland.
Wrobel, Michał Hubert
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland.
Hojo, Takuo
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland.
  • Kyushu Okinawa Agricultural Research Center, NARO, 2421, Suya, Koshi, Kumamoto, 861-1192, Japan.
Ferreira-Dias, Graca
  • Faculty of Veterinary Medicine, C.I.I.S.A, University of Lisbon, Lisbon, Portugal.
  • AL4AnimalS-Associate Laboratory for Animal and Veterinary Sciences, Lisbon, Portugal.
Skarzynski, Dariusz Jan
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland. d.skarzynski@pan.olsztyn.pl.

MeSH Terms

  • Animals
  • Horses
  • Female
  • Horse Diseases / physiopathology
  • Horse Diseases / genetics
  • Myometrium / drug effects
  • Myometrium / metabolism
  • Receptors, Lysophosphatidic Acid / genetics
  • Receptors, Lysophosphatidic Acid / metabolism
  • Lysophospholipids / pharmacology
  • RNA, Messenger / metabolism
  • RNA, Messenger / genetics
  • Uterine Contraction / drug effects
  • Endometriosis / veterinary
  • Endometriosis / genetics
  • Estrous Cycle / drug effects

Grant Funding

  • 2011/02/A/NZ5/00338 / Narodowe Centrum Nauki
  • BPN/BPT/2021/1/00026/U/00001 / Narodowa Agencja Wymiany Akademickiej

Conflict of Interest Statement

Declarations. Ethics approval and consent to participate: All material collection procedures were approved by the Local Ethics Committee for Experiments on Animals in Olsztyn, Poland (Agreements No. 51/2011). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.

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