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Home / Equine Research Bank / Molecules / The Effect of Methyl-β-cyclodextrin on Apoptosis, Prol...
Molecules (Basel, Switzerland)2018; 23(2); 287; doi: 10.3390/molecules23020287

The Effect of Methyl-β-cyclodextrin on Apoptosis, Proliferative Activity, and Oxidative Stress in Adipose-Derived Mesenchymal Stromal Cells of Horses Suffering from Metabolic Syndrome (EMS).

Abstract: Methyl-β-cyclodextrin (MβCD) is a cyclic oligosaccharide, commonly used as a pharmacological agent to deplete membrane cholesterol. In this study, we examined the effect of MβCD on adipose-derived mesenchymal stromal cells (ASCs) isolated form healthy horses (ASC) and from horses suffering from metabolic syndrome (ASC). We investigated the changes in the mRNA levels of the glucose transporter 4 (GLUT4) and found that MβCD application may lead to a significant improvement in glucose transport in ASC. We also showed that MβCD treatment affected GLUT4 upregulation in an insulin-independent manner via an NO-dependent signaling pathway. Furthermore, the analysis of superoxide dismutase activity (SOD) and reactive oxygen species (ROS) levels showed that MβCD treatment was associated with an increased antioxidant capacity in ASC. Moreover, we indicated that methyl-β-cyclodextrin treatment did not cause a dysfunction of the endoplasmic reticulum and lysosomes. Thereby, we propose the possibility of improving the functionality of ASC by increasing their metabolic stability.
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Publication Date: 2018-01-30 PubMed ID: 29385746PubMed Central: PMC6017619DOI: 10.3390/molecules23020287Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the impact of methyl-β-cyclodextrin (MβCD), a drug used to reduce cholesterol, on the health and function of adipose-derived mesenchymal stromal cells (ASCs) in horses, particularly those suffering from metabolic syndrome. The study provides optimism by suggesting MβCD treatment may improve glucose transport in ASCs, enhance antioxidant capacity, and improve metabolic stability without damaging specific cell structures.

Overview of the Research

  • The purpose of this study is to understand the influence of methyl-β-cyclodextrin (MβCD) on adipose-derived mesenchymal stromal cells (ASCs) in horses, especially those with metabolic syndrome.
  • MβCD is a cyclic oligosaccharide and is widely used as a pharmacological agent to remove cholesterol from cell membranes.
  • The researchers were interested in how MβCD affects the health and performance of ASCs in horses.

Effect on Glucose Transporter 4

  • The study particularly investigates the impact on glucose transporter 4 (GLUT4), a protein involved in the movement of glucose across cell membranes.
  • The researchers found that treatment with MβCD potentially improves the transportation of glucose in the ASCs, contributing to cell health and function.
  • Fascinatingly, this GLUT4 upregulation occurs in an insulin-independent manner, which means that MβCD treatment affects GLUT4 activity regardless of the presence or absence of insulin.
  • This GLUT4 upregulation is achieved through an NO-dependent signaling pathway, involving Nitric Oxide, a potent vasodilator.

Antioxidant Capacity and Cell Dysfunction

  • The researchers also evaluated superoxide dismutase activity (SOD) and reactive oxygen species (ROS) levels associated with antioxidant activity.
  • Increased SOD activity and ROS levels indicated that MβCD treatment is linked to higher antioxidant capacity in ASCs, improving the ability to protect against oxidative stress.
  • Along with the effect on GLUT4 and antioxidant capacity, researchers found no evidence that MβCD treatment would cause disruption to the endoplasmic reticulum and lysosomes—critical components of cell function.

Implications of the Study

  • The study hence suggests that the use of MβCD could lead to improved functionality of ASCs, especially by enhancing their metabolic stability.
  • Although the research is focused on horses, findings related to cellular function and the effects of MβCD may transfer across species, providing insights for human medicine as well.

Cite This Article

APA
Szydlarska J, Weiss C, Marycz K. (2018). The Effect of Methyl-β-cyclodextrin on Apoptosis, Proliferative Activity, and Oxidative Stress in Adipose-Derived Mesenchymal Stromal Cells of Horses Suffering from Metabolic Syndrome (EMS). Molecules, 23(2), 287. https://doi.org/10.3390/molecules23020287

Publication

Molecules (Basel, Switzerland)
ISSN: 1420-3049
NlmUniqueID: 100964009
Country: Switzerland
Language: English
Volume: 23
Issue: 2
PII: 287

Researcher Affiliations

Szydlarska, Joanna
  • Department of Experimental Biology and Electron Microscope Facility, The Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, 50-631 Wroclaw, Poland. joanna.szydlarska@gmail.com.
Weiss, Christine
  • Department of Experimental Biology and Electron Microscope Facility, The Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, 50-631 Wroclaw, Poland. d.weiss@horsedoc.ch.
Marycz, Krzysztof
  • Department of Experimental Biology and Electron Microscope Facility, The Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, 50-631 Wroclaw, Poland. krzysztofmarycz@interia.pl.
  • Wroclaw Research Centre EIT+, 54-066 Wroclaw, Poland. krzysztofmarycz@interia.pl.

MeSH Terms

  • Adipose Tissue / metabolism
  • Adipose Tissue / pathology
  • Animals
  • Cell Proliferation / drug effects
  • Horse Diseases / metabolism
  • Horse Diseases / pathology
  • Horses
  • Mesenchymal Stem Cells / metabolism
  • Mesenchymal Stem Cells / pathology
  • Metabolic Syndrome / metabolism
  • Metabolic Syndrome / pathology
  • Metabolic Syndrome / veterinary
  • Oxidative Stress / drug effects
  • beta-Cyclodextrins / pharmacology

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 1 times.
  1. Ferreira-Baptista C, Queirós A, Ferreira R, Fernandes MH, Colaço B, Gomes PS. The Osteogenic Potential of Falciform Ligament-Derived Stromal Cells-A Comparative Analysis between Two Osteogenic Induction Programs.. Bioengineering (Basel) 2022 Dec 15;9(12).
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