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Home / Equine Research Bank / Animals (Basel) / The Effect of Treatment with Oestradiol Benzoate on Oestrus ...
Animals : an open access journal from MDPI2023; 13(5); doi: 10.3390/ani13050938

The Effect of Treatment with Oestradiol Benzoate on Oestrus Expression and Endometrial Oedema in Anovulatory and Cyclic Mares.

Abstract: Oestrogens treatment is often used to induce oestrus behaviour in anoestrous mares to aid in the collection of stallion semen and as recipient mares to receive embryos when combined with progesterone. However, there are no studies to describe the effect of dose and individual mare on the intensity and duration of the response, in both anoestrous and cyclic mares. In Experiment 1, 13 anoestrous mares were treated with one of five doses of oestradiol benzoate (OB) (1, 1.5, 2, 3 and 4 mg) per mare in five consecutive treatment periods (n = 65), to determine the response in terms of endometrial oedema and oestrous behaviour. Experiment 2 and 3 used 3 mg of OB in cyclic mares to confirm or deny the presence of an active corpus luteum (CL). There was a dose rate of OB and individual mare effect (p < 0.05) on the intensity and persistence of endometrial oedema and oestrous behaviour. A total of 2 mg OB was enough to induce endometrial oedema and oestrous behaviour within 48 h in most mares. Mares with an active CL did not show endometrial oedema following treatment of 3 mg OB.
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Publication Date: 2023-03-05 PubMed ID: 36899795PubMed Central: PMC10000200DOI: 10.3390/ani13050938Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on understanding the effect of different doses of the hormone oestradiol benzoate on inducing oestrus behaviour and endometrial oedema in anovulatory and cyclic mares for the convenience of semen collection and embryo transfer.

Objective of the Study

The primary aim of this study was to determine the impact of various doses of oestradiol benzoate (OB), a type of estrogen, on the initiation of oestrus behaviour and induction of endometrial oedema in both anovulatory (not ovulating) and cyclic (ovulating regularly) mares. There was also an interest in the investigation of the intensity and duration of the response, which has been previously unstudied.

Methodology

In the first experiment:

  • 13 anovulatory mares were administered one of the five predetermined doses of OB (1, 1.5, 2, 3, and 4 mg) in five following treatment cycles (totalling 65 administrations).
  • The response, in terms of endometrial oedema (swelling of the uterus lining) and oestrous behaviour (actions indicating readiness for mating), was observed and recorded.

In the second and third experiments:

  • 3 mg of OB was administered to cyclic mares.
  • The presence or absence of a corpus luteum (CL, the structure that develops after ovulation) was noted.

Results

From the results it was clear that OB dose rate and individual mare attributes greatly influenced the severity and consistency of endometrial oedema and oestrous behaviour.

  • It was found that 2 mg OB was enough to induce endometrial oedema and oestrous behaviour within 48 hours in most mares.
  • Mares with an active CL did not display endometrial oedema when treated with 3 mg of OB, possibly indicating a hormonal resistance during the active luteal phase.

In essence, this study provides pivotal data on the application of hormone therapy in breeding and veterinary practices, setting guidelines on the optimal treatment dosage for inducing oestrus behaviour in mares.

Cite This Article

APA
Silva ESM, Newcombe JR, Cuervo-Arango J. (2023). The Effect of Treatment with Oestradiol Benzoate on Oestrus Expression and Endometrial Oedema in Anovulatory and Cyclic Mares. Animals (Basel), 13(5). https://doi.org/10.3390/ani13050938

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 13
Issue: 5

Researcher Affiliations

Silva, Elisa S M
  • Faculty of Veterinary Medicine, Federal University of Uberlandia, Uberlândia 38408-100, Brazil.
Newcombe, John R
  • Warren House Farm, Equine Fertility Clinic, Brownhills WS8 6LU, UK.
Cuervo-Arango, Juan
  • Equine Fertility Group, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46115 Valencia, Spain.

Conflict of Interest Statement

The authors declare no conflict of interest.

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