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Inflammation research : official journal of the European Histamine Research Society ... [et al.]2014; 63(8); 637-647; doi: 10.1007/s00011-014-0736-2

The effect of tumour necrosis factor-α and insulin on equine digital blood vessel function in vitro.

Abstract: Insulin and inflammatory cytokines may be involved in equine laminitis, which might be associated with digital vascular dysfunction. This study determined the effects of TNF-α and insulin on the endothelial-dependent relaxant responses of equine digital blood vessels and on equine digital vein endothelial cell (EDVEC) cGMP production. Methods: Isolated rings of equine digital arteries (EDAs) and veins (EDVs) were obtained and EDVECs were cultured from horses euthanized at an abattoir. Methods: The effect of incubation with TNF-α (10 ng/ml) and/or insulin (1,000 μIU/ml) for 1.5 h or overnight under hyperoxic and hypoxic conditions on carbachol (endothelium-dependent) induced relaxation was assessed. The time course and concentration dependency of the effect of TNF-α and the effect of insulin (1,000 μIU/ml) on EDVEC cGMP production was determined. Results: Incubation of EDAs overnight with TNF-α under hypoxic conditions resulted in endothelial-dependent vascular dysfunction. EDVs produced a more variable response. TNF-α increased EDVEC cGMP formation in a time- and concentration-dependent manner. Insulin had no significant effects. Conclusions: There is a mismatch between the results obtained from isolated vessel rings and cultured endothelial cells suggesting TNF-α may reduce the biological effect of NO by reducing its bioavailability rather than its formation, leading to endothelial cell dysregulation.
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Publication Date: 2014-04-24 PubMed ID: 24764104DOI: 10.1007/s00011-014-0736-2Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research looked into the effects of insulin and tumour necrosis factor-alpha (TNF-α), a protein involved in inflammation, on the functioning of blood vessels in the horse hoof. The results found a negative impact on the vessels when TNF-α was present, suggesting a possible role in the development of a horse disease known as laminitis.

Research Methodology

  • Various portions of equine digital arteries (EDAs) and veins (EDVs) were obtained from horses euthanized at an abattoir.
  • The extracted vessels were then treated with TNF-α and/or insulin for different times under various oxygen conditions.
  • The effect of TNF-α and insulin was assessed on the vasculature dependent relaxation induced by carbachol, a medication that stimulates the parasympathetic nervous system.
  • The study also determined the time course and concentration dependency of the effect of TNF-α and insulin on equine digital vein endothelial cell (EDVEC) cGMP (a messenger molecule) production.

Research Findings

  • Treatment of equine digital arteries with TNF-α under oxygen-deprived conditions caused a significant vascular dysfunction, which shows TNF-α can negatively affect the normal functioning of the vessels.
  • The study exhibited a more inconsistent response in digital veins.
  • TNF-α was found to augment the formation of EDVEC cGMP in a time- and concentration-dependent way.
  • Contrary to TNF-α, insulin had no appreciable impact on EDVEC cGMP production.

Conclusions

  • There was a difference between the results obtained from the experiments using detached vessel rings and the cultured endothelial cells.
  • This discrepancy indicates that TNF-α may decrease the biological effect of a molecule called Nitric Oxide (NO) by lowering its availability, rather than its formation.
  • This could consequently lead to poor regulation of endothelial cells’ functioning thereby leading to vascular dysfunction, which may play a role in the tendency towards digital laminitis in horses.

Cite This Article

APA
Menzies-Gow NJ, Wray H, Bailey SR, Harris PA, Elliott J. (2014). The effect of tumour necrosis factor-α and insulin on equine digital blood vessel function in vitro. Inflamm Res, 63(8), 637-647. https://doi.org/10.1007/s00011-014-0736-2

Publication

Inflammation research : official journal of the European Histamine Research Society ... [et al.]
ISSN: 1420-908X
NlmUniqueID: 9508160
Country: Switzerland
Language: English
Volume: 63
Issue: 8
Pages: 637-647

Researcher Affiliations

Menzies-Gow, Nicola J
  • Royal Veterinary College, London, UK, nmenziesgow@rvc.ac.uk.
Wray, H
    Bailey, S R
      Harris, P A
        Elliott, J

          MeSH Terms

          • Animals
          • Arteries / drug effects
          • Arteries / physiology
          • Carbachol
          • Cells, Cultured
          • Cyclic GMP / metabolism
          • Endothelial Cells / drug effects
          • Endothelial Cells / metabolism
          • Horses
          • Hyperoxia / physiopathology
          • Hypoxia / physiopathology
          • In Vitro Techniques
          • Insulin / pharmacology
          • Metatarsophalangeal Joint
          • Tumor Necrosis Factor-alpha / pharmacology
          • Vasodilation / drug effects
          • Veins / drug effects
          • Veins / physiology

          References

          This article includes 89 references
          1. Am J Physiol Heart Circ Physiol. 2005 Aug;289(2):H813-22
            pubmed: 15792994
          2. Eur J Pharmacol. 2007 Aug 27;569(3):212-21
            pubmed: 17560569
          3. Vet Immunol Immunopathol. 2006 Jan 15;109(1-2):161-6
            pubmed: 16169600
          4. Arterioscler Thromb Vasc Biol. 2006 Feb;26(2):274-80
            pubmed: 16322532
          5. Circulation. 2003 Oct 14;108(15):1815-21
            pubmed: 14530204
          6. J Vet Pharmacol Ther. 2013 Aug;36(4):382-8
            pubmed: 22943152
          7. J Appl Physiol (1985). 1993 Dec;75(6):2392-9
            pubmed: 8125855
          8. Am J Vet Res. 2007 Jul;68(7):722-9
            pubmed: 17605607
          9. Diabetes Res Clin Pract. 2007 Sep;77 Suppl 1:S161-4
            pubmed: 17481767
          10. J Am Vet Med Assoc. 2004 Apr 1;224(7):1123-7
            pubmed: 15074858
          11. Equine Vet J. 1999 May;31(3):212-8
            pubmed: 10402134
          12. Eur J Pharmacol. 1995 Jan 24;273(1-2):121-8
            pubmed: 7537677
          13. Equine Vet J. 2011 Jan;43(1):54-61
            pubmed: 21143634
          14. Equine Vet J. 2013 Sep;45(5):613-8
            pubmed: 23489109
          15. J Vet Pharmacol Ther. 2013 Jun;36(3):267-74
            pubmed: 22762272
          16. Can J Vet Res. 2006 Jul;70(3):197-205
            pubmed: 16850942
          17. Circ Res. 1993 Jul;73(1):205-9
            pubmed: 7685252
          18. Eur J Pharmacol. 1998 Aug 21;355(2-3):175-87
            pubmed: 9760032
          19. Am J Physiol Heart Circ Physiol. 2002 Nov;283(5):H1785-94
            pubmed: 12384455
          20. Circulation. 2006 Apr 18;113(15):1888-904
            pubmed: 16618833
          21. Brain. 2002 Nov;125(Pt 11):2446-59
            pubmed: 12390971
          22. Biochem Soc Trans. 2004 Feb;32(Pt 1):110-2
            pubmed: 14748725
          23. Equine Vet J. 1994 Sep;26(5):378-84
            pubmed: 7988541
          24. Brain Res Bull. 1996;39(4):241-7
            pubmed: 8963690
          25. Am J Physiol Cell Physiol. 2001 May;280(5):C1057-65
            pubmed: 11287317
          26. J Am Vet Med Assoc. 2006 May 15;228(10):1538-45
            pubmed: 16677122
          27. J Vet Intern Med. 2013 Jan-Feb;27(1):157-63
            pubmed: 23216530
          28. Horm Metab Res. 1997 Mar;29(3):111-4
            pubmed: 9137980
          29. J Am Vet Med Assoc. 1970 Jan 1;156(1):76-83
            pubmed: 5460644
          30. J Vet Pharmacol Ther. 1998 Feb;21(1):74-81
            pubmed: 9507461
          31. Mol Cell Biol. 1997 Oct;17(10):5719-26
            pubmed: 9315630
          32. Am J Physiol Heart Circ Physiol. 2000 Sep;279(3):H946-51
            pubmed: 10993754
          33. Am J Vet Res. 2008 Jan;69(1):122-9
            pubmed: 18167097
          34. Vet Immunol Immunopathol. 2009 Jun 15;129(3-4):216-20
            pubmed: 19108899
          35. Am J Vet Res. 1994 Apr;55(4):551-5
            pubmed: 8017702
          36. Diabet Med. 1999 Mar;16(3):207-11
            pubmed: 10227565
          37. Equine Vet J. 1990 Nov;22(6):416-21
            pubmed: 2269265
          38. J Clin Invest. 1995 Mar;95(3):1086-91
            pubmed: 7883956
          39. Arterioscler Thromb Vasc Biol. 2010 Nov;30(11):2156-63
            pubmed: 20814014
          40. Vet Immunol Immunopathol. 2006 Apr 15;110(3-4):195-206
            pubmed: 16290066
          41. Am J Vet Res. 1998 Jul;59(7):814-7
            pubmed: 9659543
          42. Circ Res. 2006 Jul 7;99(1):69-77
            pubmed: 16741160
          43. Cardiovasc Diabetol. 2012 Jun 18;11:72
            pubmed: 22709426
          44. Neurosci Lett. 1992 Dec 14;148(1-2):137-40
            pubmed: 1300486
          45. J Clin Invest. 1996 Aug 15;98(4):894-8
            pubmed: 8770859
          46. Lancet. 1997 Apr 26;349(9060):1210-3
            pubmed: 9130942
          47. Am Surg. 1998 Mar;64(3):203-9; discussion 209-10
            pubmed: 9520808
          48. Am J Vet Res. 2000 Aug;61(8):862-8
            pubmed: 10951973
          49. Am J Physiol Endocrinol Metab. 2009 Sep;297(3):E568-77
            pubmed: 19491294
          50. Equine Vet J. 2003 Mar;35(2):164-9
            pubmed: 12638793
          51. Endocr Rev. 2007 Aug;28(5):463-91
            pubmed: 17525361
          52. Am J Physiol. 1998 Nov;275(5):H1592-8
            pubmed: 9815065
          53. Nature. 1980 Nov 27;288(5789):373-6
            pubmed: 6253831
          54. Hypertens Pregnancy. 2008;27(1):29-38
            pubmed: 18293202
          55. Am J Vet Res. 1989 Apr;50(4):508-17
            pubmed: 2712417
          56. J Appl Physiol (1985). 2006 Mar;100(3):759-63
            pubmed: 16269525
          57. J Biol Chem. 2004 Jan 9;279(2):963-9
            pubmed: 14581470
          58. Am J Vet Res. 2003 Nov;64(11):1438-43
            pubmed: 14620782
          59. Chest. 1991 Oct;100(4):1133-7
            pubmed: 1914573
          60. J Vet Pharmacol Ther. 2003 Oct;26(5):361-8
            pubmed: 14633189
          61. Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2691-5
            pubmed: 7708707
          62. Neurosci Lett. 1994 Feb 28;168(1-2):65-8
            pubmed: 7518067
          63. J Vet Pharmacol Ther. 1997 Aug;20(4):308-17
            pubmed: 9280371
          64. Nature. 2006 Apr 13;440(7086):944-8
            pubmed: 16612386
          65. Vet J. 2007 Nov;174(3):530-5
            pubmed: 17719811
          66. Equine Vet J. 2011 Nov;43(6):744-9
            pubmed: 21496102
          67. Am J Vet Res. 1976 Oct;37(10):1171-6
            pubmed: 984542
          68. Am J Vet Res. 2012 Jul;73(7):1047-56
            pubmed: 22738057
          69. Am J Vet Res. 2003 Sep;64(9):1124-31
            pubmed: 13677390
          70. Clin Sci (Lond). 2009 Feb;116(3):219-30
            pubmed: 19118493
          71. Br J Anaesth. 2004 Jul;93(1):105-13
            pubmed: 15121728
          72. Circ Res. 2009 Jun 5;104(11):1275-82
            pubmed: 19423845
          73. Equine Vet J. 2007 Jan;39(1):42-7
            pubmed: 17228594
          74. Domest Anim Endocrinol. 2011 Oct;41(3):111-7
            pubmed: 21696910
          75. Vet Immunol Immunopathol. 2009 Jun 15;129(3-4):231-41
            pubmed: 19131116
          76. Equine Vet J. 1998 Mar;30(2):124-30
            pubmed: 9535068
          77. Equine Vet J. 2014 May;46(3):317-21
            pubmed: 23819851
          78. J Huazhong Univ Sci Technolog Med Sci. 2012 Oct;32(5):642-647
            pubmed: 23073791
          79. Equine Vet J Suppl. 1998 Sep;(26):125-32
            pubmed: 9932103
          80. Equine Vet J. 1996 Jan;28(1):38-46
            pubmed: 8565952
          81. Equine Vet J. 2010 Mar;42(2):129-35
            pubmed: 20156248
          82. Vet J. 2004 Mar;167(2):129-42
            pubmed: 14975387
          83. Am J Physiol Heart Circ Physiol. 2007 Aug;293(2):H1115-21
            pubmed: 17496212
          84. Am J Vet Res. 2003 Jun;64(6):708-14
            pubmed: 12828256
          85. J Anim Sci. 2007 May;85(5):1144-55
            pubmed: 17264235
          86. Stroke. 1998 Feb;29(2):509-15
            pubmed: 9472897
          87. Vitam Horm. 2006;74:443-77
            pubmed: 17027526
          88. Arterioscler Thromb Vasc Biol. 1995 Jan;15(1):52-7
            pubmed: 7538424
          89. Am J Vet Res. 2001 Jul;62(7):1167-72
            pubmed: 11453497

          Citations

          This article has been cited 2 times.
          1. Zamith Cunha R, Gobbo F, Morini M, Salamanca G, Zanoni A, Bernardini C, Gramenzi A, Chiocchetti R. Cannabinoid and cannabinoid related receptors in fibroblasts, inflammatory and endothelial cells of the equine hoof with and without laminitis: novel pharmacological target. Front Vet Sci 2025;12:1723160.
            doi: 10.3389/fvets.2025.1723160pubmed: 41394912google scholar: lookup
          2. Hobbs KJ, Bayless R, Sheats MK. A Comparative Review of Cytokines and Cytokine Targeting in Sepsis: From Humans to Horses. Cells 2024 Sep 5;13(17).
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