FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Clin Exp Dermatol / The effects of equine peripheral blood stem cells on cutaneo...
Clinical and experimental dermatology2013; 38(3); 280-284; doi: 10.1111/ced.12068

The effects of equine peripheral blood stem cells on cutaneous wound healing: a clinical evaluation in four horses.

Abstract: Stem-cell therapy represents a promising strategy for the treatment of challenging pathologies, such as large, infected wounds that are unresponsive to conventional therapies. The present study describes the clinical application of peripheral blood stem cells (PBSCs) for the treatment of four adult Warmblood horses with naturally occurring wounds, which were unresponsive to conventional therapies for at least 3 months. A visual assessment was performed, and a number of wound-healing parameters (granulation tissue, crust formation and scar formation) were evaluated. In all cases, tissue overgrowth was visible within 4 weeks after PBSC injection, followed by the formation of crusts and small scars in the centre of the wound, with hair regeneration at the edges. In conclusion, this is the first report of PBSC therapy of skin wounds in horses, and it produced a positive visual and clinical outcome.
© The Author(s) CED © 2013 British Association of Dermatologists.
Get Full Text
Publication Date: 2013-03-23 PubMed ID: 23517358PubMed Central: PMC3627309DOI: 10.1111/ced.12068Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Case Reports
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article presents a study on the application of peripheral blood stem cells (PBSCs) in the treatment of persistent wounds in four adult Warmblood horses, demonstrating promising results in tissue regeneration and improved wound healing.

Objective of the Research

  • The main purpose of this study is to explore the therapeutic potential of peripheral blood stem cells (PBSCs) in healing persistent, non-healing, naturally occurring wounds in adult Warmblood horses, which hadn’t responded to conventional treatment for at least three months.

Methodology and Approach

  • The researchers evaluated the effects of PBSC therapy on wound healing by assessing a number of parameters including the construction of granulation tissue, formation of crusts, scar generation, and hair regeneration at the edges of the wound.
  • A visual assessment of the wound healing process was also carried out to visually observe the changes over time.

Findings of the Research

  • The study found that, in all cases, there was visible tissue overgrowth within 4 weeks after the injection of PBSCs into the wound site. This was followed by the development of crusts and small scars in the center of the wound.
  • Also, hair began to regrow at the edges of the wounds, indicating a progression in the wound healing process.

Conclusions from the Research

  • This research presents the first detailed report on the use of peripheral blood stem cell therapy for skin wounds in horses. The application of this type of stem cell therapy demonstrated a positive outcome both visually and clinically.
  • The results suggest that PBSC therapy could be an effective alternative solution for healing large, challenging, and infected wounds that don’t respond to traditional treatment methods.

Cite This Article

APA
Spaas JH, Broeckx S, Van de Walle GR, Polettini M. (2013). The effects of equine peripheral blood stem cells on cutaneous wound healing: a clinical evaluation in four horses. Clin Exp Dermatol, 38(3), 280-284. https://doi.org/10.1111/ced.12068

Publication

Clinical and experimental dermatology
ISSN: 1365-2230
NlmUniqueID: 7606847
Country: England
Language: English
Volume: 38
Issue: 3
Pages: 280-284

Researcher Affiliations

Spaas, J H
  • Department of Comparative Physiology and Biometrics, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Broeckx, S
    Van de Walle, G R
      Polettini, M

        MeSH Terms

        • Animals
        • Female
        • Horses / injuries
        • Leg Injuries / therapy
        • Leg Injuries / veterinary
        • Male
        • Peripheral Blood Stem Cell Transplantation / veterinary
        • Skin / injuries
        • Wound Healing / physiology

        References

        This article includes 23 references
        1. Funkquist B, Obel N. Fixation of skin grafts in the horse using stainless steel staples.. Equine Vet J 1979;11:117–21.
          pubmed: 383478
        2. Bristol DG. Skin grafts and skin flaps in the horse.. Vet Clin North Am Equine Pract 2005;21:125–44.
          pubmed: 15691604
        3. Wilmink JM, Stolk PW, Van Weeren PR, Barneveld A. Differences in second-intention wound healing between horses and ponies: macroscopic aspects.. Equine Vet J 1999;31:53–60.
          pubmed: 9952330
        4. Jacobs KA, Leach DH, Fretz PB, Townsend HGG. Comparative aspects of the healing of excisional wounds on the leg and body of horses.. Vet Surg 1984;13:83–90.
        5. Bigbie RB, Schumacher J, Swaim SF. Effects of amnion and live yeast cell derivative on second-intention healing in horses.. Am J Vet Res 1991;52:1376–82.
          pubmed: 1928923
        6. Schwartz AJ, Wilson DA, Keegan KG. Factors regulating collagen synthesis and degradation during second-intention healing of wounds in the thoracic region and the distal aspect of the forelimb of horses.. Am J Vet Res 2002;63:1564–70.
          pubmed: 12428668
        7. Turner TD. Interactive dressings used in the management of human soft tissue injuries and their potential in veterinary practice.. Vet Dermatol 1997;8:235–42.
          pubmed: 34645021
        8. Wilmink JM, van Herten J, van Weeren PR, Barneveld A. Retrospective study of primary intention healing and sequestrum formation in horses compared to ponies under clinical circumstances.. Equine Vet J 2002;34:270–3.
          pubmed: 12108745
        9. Theoret CL, Barber SM, Moyana TN, Gordon JR. Expression of transforming growth factor beta (1), beta (3), and basic fibroblast growth factor in full-thickness skin wounds of equine limbs and thorax.. Vet Surg 2001;30:269–77.
          pubmed: 11340559
        10. Toth F, Schumacher J, Castro F, Perkin J. Full-thickness skin grafting to cover equine wounds caused by laceration or tumor resection.. Vet Surg 2010;39:708–14.
          pubmed: 20459503
        11. Booth LC. Equine wound reconstruction using free skin grafting.. Calif Vet 1991;45:13–6.
        12. Wilmink JM, Van den Boom R, van Weeren PR, Barneveld A. The modified Meek technique as a novel method for skin grafting in horses: evaluation of acceptance, wound contraction and closure in chronic wounds.. Equine Vet J 2006;38:324–9.
          pubmed: 16866199
        13. Rapaport FT, Thomas L, Converse JM, Lawrence JM. The specificity of skin homograft rejection in man.. Ann New York Acad Sciences 1960;87:217–22.
          pubmed: 13739547
        14. Gomez JH, Schumacher J, Lauten SD. Effects of 3 biologic dressings on healing of cutaneous wounds on the limbs of horses.. Can J Vet Res 2004;68:49–55.
          pmc: PMC1142129pubmed: 14979435
        15. Hanpanich BS. Tissue engineering of skin and soft tissue augmentation, medical view.. J Med Assoc Thai 2010;93(Suppl 7):S332–6.
          pubmed: 21294434
        16. Cotran SC, Kumare V, Robbins SL. Cellular growth and differentiation: normal regulation and adaptations: inflammation and repair.. .
        17. Karp JM, Leng Teo GS. Mesenchymal stem cell homing: the devil is in the details.. Cell Stem Cell 2009;4:206–16.
          pubmed: 19265660
        18. Jain P, Perakath B, Jesudason MR, Nayak S. The effect of autologous bone marrow-derived cells on healing chronic lower extremity wounds: results of a randomized controlled study.. Ostomy Wound Manage 2011;57:38–44.
          pubmed: 21904014
        19. Marfe G, Massaro-Giordano M, Ranalli M. Blood derived stem cells: an ameliorative therapy in veterinary ophthalmology.. J Cell Physiol 2012;227:1250–6.
          pubmed: 21792938
        20. Spaas JH, Gambacurta A, Polettini M. Purification and expansion of stem cells from equine peripheral blood, with clinical applications.. Vlaams Diergeneeskundig Tijdschrift 2011;80:129–35.
        21. Carrade DD, Affolter VK, Outerbridge CA. Intradermal injections of equine allogeneic umbilical cord-derived mesenchymal stem cells are well tolerated and do not elicit immediate or delayed hypersensitivity reactions.. Cytotherapy 2011;13:1180–92.
          pubmed: 21899391
        22. Berry DB, Sullins KE. Effects of topical application of antimicrobials and bandaging on healing and granulation tissue formation in wounds of the distal aspect of the limbs in horses.. Am J Vet Res 2003;64:88–92.
          pubmed: 12518884
        23. Theoret CL. Physiology of wound healing.. .

        Citations

        This article has been cited 9 times.
        1. Ribeiro G, Carvalho L, Borges J, Prazeres J. The Best Protocol to Treat Equine Skin Wounds by Second Intention Healing: A Scoping Review of the Literature. Animals (Basel) 2024 May 18;14(10).
          doi: 10.3390/ani14101500pubmed: 38791717google scholar: lookup
        2. El-Husseiny HM, Mady EA, Helal MAY, Tanaka R. The Pivotal Role of Stem Cells in Veterinary Regenerative Medicine and Tissue Engineering. Vet Sci 2022 Nov 21;9(11).
          doi: 10.3390/vetsci9110648pubmed: 36423096google scholar: lookup
        3. Jørgensen E, Bjarnsholt T, Jacobsen S. Biofilm and Equine Limb Wounds. Animals (Basel) 2021 Sep 27;11(10).
          doi: 10.3390/ani11102825pubmed: 34679846google scholar: lookup
        4. Cequier A, Sanz C, Rodellar C, Barrachina L. The Usefulness of Mesenchymal Stem Cells beyond the Musculoskeletal System in Horses. Animals (Basel) 2021 Mar 25;11(4).
          doi: 10.3390/ani11040931pubmed: 33805967google scholar: lookup
        5. Voga M, Adamic N, Vengust M, Majdic G. Stem Cells in Veterinary Medicine-Current State and Treatment Options. Front Vet Sci 2020;7:278.
          doi: 10.3389/fvets.2020.00278pubmed: 32656249google scholar: lookup
        6. Lanci A, Merlo B, Mariella J, Castagnetti C, Iacono E. Heterologous Wharton's Jelly Derived Mesenchymal Stem Cells Application on a Large Chronic Skin Wound in a 6-Month-Old Filly. Front Vet Sci 2019;6:9.
          doi: 10.3389/fvets.2019.00009pubmed: 30761313google scholar: lookup
        7. Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H, Canciello A, Greco L, Muttini A, Gatta V, Stuppia L, Mattioli M. Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance. Cell Transplant 2018 Jan;27(1):93-116.
          doi: 10.1177/0963689717724797pubmed: 29562773google scholar: lookup
        8. Textor JA, Clark KC, Walker NJ, Aristizobal FA, Kol A, LeJeune SS, Bledsoe A, Davidyan A, Gray SN, Bohannon-Worsley LK, Woolard KD, Borjesson DL. Allogeneic Stem Cells Alter Gene Expression and Improve Healing of Distal Limb Wounds in Horses. Stem Cells Transl Med 2018 Jan;7(1):98-108.
          doi: 10.1002/sctm.17-0071pubmed: 29063737google scholar: lookup
        9. Park JW, Hwang SR, Yoon IS. Advanced Growth Factor Delivery Systems in Wound Management and Skin Regeneration. Molecules 2017 Jul 27;22(8).
          doi: 10.3390/molecules22081259pubmed: 28749427google scholar: lookup
        Subscribe to our newsletter
        Join 99,357 horse owners like you who receive our email updates on horse health and nutrition.