The ELY-1 locus controls a di-allelic alloantigenic system on equine lymphocytes.
Abstract: The ELY-1 locus controls the expression of a polymorphic cell surface antigen of equine lymphocytes which was detected using antibodies generated by alloimmunization with peripheral blood lymphocytes. The ELY-1 antigens were not detected on erythrocytes or platelets by absorption experiments. The two alleles, which have been designated ELY-1.1 and ELY-1.2, are expressed codominantly and appear to constitute a closed system at the population level. In family studies, the ELY-1 antigens segregated as products of an autosomal locus not linked to the major histocompatibility complex (MHC) of the horse. In the complement mediated lymphocyte microcytotoxicity test, antisera to the ELY-1 antigens selectively killed peripheral blood lymphocytes which did not express surface immunoglobulin. The ELY-1 antigens may be useful markers for equine T cells when assayed in this fashion. Three alloantisera were used in immune precipitation of iodinated and solubilized cell surface proteins from peripheral blood lymphocytes. Electrophoresis of the precipitates in sodium dodecyl sulphate (SDS)-polyacrylamide gels demonstrated strong bands in the Mr 180-190K range that were shared in the three different preparations. These results suggest that the ELY-1 allospecificities are expressed on an equine equivalent of the murine T200 molecule.
Publication Date: 1987-02-01 PubMed ID: 3655400DOI: 10.1111/j.1744-313x.1987.tb00363.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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This research explores how the ELY-1 locus controls the expression of a variable antigen on horse lymphocytes, with potential uses as markers for equine T cells.
Overview of the Research
- This research was focused on understanding the role of a locus called ELY-1 in controlling the expression of a cell surface antigen on equine (horse) lymphocytes.
- Lymphocytes are a type of white blood cell that plays a large role in the immune response of an organism.
- The antigen was detected using antibodies generated by alloimmunization with peripheral blood lymphocytes. Alloimmunization refers to the process where an individual is exposed to antigens from another member of the same species, causing an immune response and resulting in the generation of antibodies against those antigens.
Findings of the Research
- The researchers found that the ELY-1 antigens did not appear on erythrocytes (red blood cells) or platelets.
- Two variations or alleles of ELY-1, named ELY-1.1 and ELY-1.2, were identified. It was found that these alleles are expressed codominantly and seem to constitute a closed system at the population level.
- In family-based studies, these ELY-1 antigens behaved as if they were products of an autosomal locus not linked to the horse’s Major Histocompatibility Complex (MHC). The MHC is a genetic system that allows the immune system to differentiate between self-cells and non-self cells.
Practical Implications of the Research
- The study suggests that the ELY-1 antigens could serve as useful markers for equine T cells.
- Using antisera to the ELY-1 antigens in a microcytotoxicity test, it was shown that these antibodies can selectively kill peripheral blood lymphocytes that did not express surface immunoglobulin.
- Iodinated and solubilized cell surface proteins from peripheral blood lymphocytes were used in immune precipitation, and the electrophoresis of these precipitates showed strong bands in the molecular weight range of 180-190K, suggesting that the ELY-1 specificity could be expressed on the equine equivalent of the murine (mouse) T200 molecule.
Significance of the Research
- This research adds to our understanding of the genetic underpinnings of immune response and antigen expression in horses.
- The findings could have important implications in the realm of equine health and veterinary science, particularly for diagnosing or tracking certain immune-related conditions.
Cite This Article
APA
Byrns G, Crump AL, Lalonde G, Bernoco D, Antczak DF.
(1987).
The ELY-1 locus controls a di-allelic alloantigenic system on equine lymphocytes.
J Immunogenet, 14(1), 59-71.
https://doi.org/10.1111/j.1744-313x.1987.tb00363.x Publication
Researcher Affiliations
- Department of Reproduction, School of Veterinary Medicine, University of California, Davis 95616.
MeSH Terms
- Alleles
- Animals
- Antigens, Surface / genetics
- Gene Frequency
- Horses / genetics
- Horses / immunology
- Isoantigens / genetics
- Lymphocytes / physiology
- Molecular Weight
- Polymorphism, Genetic
- Receptors, Antigen, B-Cell / analysis
Grant Funding
- HD-15799 / NICHD NIH HHS
Citations
This article has been cited 1 times.- Binns RM, Licence ST, Whyte A, Wilby M, Rothkötter HJ, Bacon M. Genetically determined CD45 variant of value in leucocyte tracing in vivo in the pig.. Immunology 1995 Sep;86(1):25-33.
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