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Home / Equine Research Bank / Histochem J / The enzyme histochemistry of developing odontoblasts in catt...
The Histochemical journal1969; 1(4); 281-294; doi: 10.1007/BF01003275

The enzyme histochemistry of developing odontoblasts in cattle, pigs and horses.

Abstract: The histochemical distribution of some hydrolytic and oxidative enzymes in developing odontoblasts and subodontoblasts in cattle, pigs and horses has been observed in cryostat sections of teeth that have been decalcified with neutral EDTA. Undifferentiated dental epithelium and immature odontoblasts of the bell stage tooth germ showed lower levels of enzymatic activity as compared with the well-developed tooth germ. When the dentine matrix began to form, the young odontoblasts appeared to have a significantly positive reaction for acid phosphatase, and gradually other enzymes developed an activity at the top of the cusp. Odontoblasts as well as subodontoblastic-rich cells showed strong enzymatic activities for hydrolytic and oxidative enzymes, that is, they were strongly reactive for alkaline and acid phosphatase and lactate and malate dehydrogenases, and moderately reactive for other oxidative enzyme systems. It is suggested that the subodontoblastic layer is concerned with the biosynthesis of dentinal matrix as well as with the odontoblasts themselves.
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Publication Date: 1969-05-01 PubMed ID: 4113284DOI: 10.1007/BF01003275Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the distribution of various hydrolytic and oxidative enzymes in the development of odontoblasts (tooth-producing cells) in various animals. The study discovered that these cells demonstrated various levels of enzymatic activity, and this variance may be critical in tooth development.

Research Methods and Observations

  • The study mainly revolved around analyzing the histochemical (chemical analysis of cells using various staining techniques) distribution of certain enzymes in developing odontoblasts. Cell development in cattle, pigs and horses were studied.
  • In the study, cryostat sections of teeth that had been decalcified with neutral EDTA were used.
  • Researchers observed that the undifferentiated dental epithelium and immature odontoblasts of the bell stage tooth germ showed lower levels of enzymatic activity as compared to the well-developed tooth germ.
  • When the dentine matrix began to form, the young odontoblasts had a significantly positive reaction for the enzyme ‘acid phosphatase’. Over time, other enzymes developed activity at the top of the cusp (pointed part of the tooth).

Key Findings

  • The findings showed that the cells showed strong enzymatic activities for hydrolytic (breaking down of a chemical compound by reaction with water) and oxidative enzymes.
  • These cells were found to be strongly reactive for alkaline and acid phosphatase and lactate and malate dehydrogenases. They were moderately reactive for other oxidative enzyme systems.
  • The results also suggested that the subodontoblastic (layer beneath the odontoblasts) layer may play a role in the biosynthesis of the dentinal matrix (hard part of the tooth) as well as with the odontoblasts themselves.

Conclusions and Implications

  • The study concluded that the enzymatic activity in the odontoblasts varied through different developmental stages. The enzymatic activity increased when the tooth structures were well-formed.
  • These observations may be indicative of the vital role these enzymes play in dental development and may aid future studies on tooth health and development. The role of the subodontoblastic layer in tooth formation was also highlighted for further exploration.

Cite This Article

APA
Mori M, Sakiyama Y. (1969). The enzyme histochemistry of developing odontoblasts in cattle, pigs and horses. Histochem J, 1(4), 281-294. https://doi.org/10.1007/BF01003275

Publication

The Histochemical journal
ISSN: 0018-2214
NlmUniqueID: 0163161
Country: Netherlands
Language: English
Volume: 1
Issue: 4
Pages: 281-294

Researcher Affiliations

Mori, M
    Sakiyama, Y

      MeSH Terms

      • Animals
      • Cattle
      • Dentin
      • Edetic Acid
      • Fetus
      • Freezing
      • Histocytochemistry
      • Horses
      • Microtomy
      • NAD
      • NADP
      • Odontoblasts / enzymology
      • Oxidoreductases / analysis
      • Species Specificity
      • Staining and Labeling
      • Swine
      • Time Factors

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      Citations

      This article has been cited 1 times.
      1. Nakai M, Tatemoto Y, Mori H, Mori M. Lectin-binding patterns in the developing tooth. Histochemistry 1985;83(5):455-63.
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