The first investigation of west nile virus in horses using real time rt-PCR in middle black sea region in Turkey.
Abstract: West Nile Virus (WNV) is a mosquito-borne disease that can cause fatal infection in mammals including humans, dogs, horses, birds and reptiles. Although West Nile Virus is an asymptomatic infection, especially it can cause neurologic disorders in humans and horses. The aim of this study was to the investigate virological presence of WNV in horses in the Black Sea Region of Turkey using real time RT-PCR (rRT-PCR). Methods: Totally, 120 horse sera were collected equally from 4 provinces in Middle Black Sea Region of Turkey and investigated for WNV presence by Taqman based rRT-PCR. Results: WNV nucleic acid was not detected in any horse serum sample. Conclusions: Although obtained result indicated no evidence of WNV-RNA in horses, Black Sea Region of Turkey is one of the suitable places for the WNV infection. For this reason, our research will continue for the determination of the viruses in vectors and susceptible animals such as horses, dogs, etc.
Publication Date: 2012-12-31 PubMed ID: 23378973PubMed Central: PMC3547302
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- Journal Article
- Diagnosis
- Disease Diagnosis
- Disease Prevalence
- Disease Surveillance
- Epidemiology
- Equine Diseases
- Equine Health
- Horses
- Infectious Disease
- Laboratory Methods
- Mosquito-borne Diseases
- Neurological Diseases
- Public Health
- Real-Time PCR
- Seroprevalence
- Vector-borne disease
- Veterinary Medicine
- Veterinary Research
- Virology
- West Nile Virus
Summary
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The research paper focuses on the investigation of the West Nile Virus (WNV) in horses in Turkey’s Middle Black Sea region using real time RT-PCR.
Research Objectives and Methods
- The main aim of this study was to investigate the presence of West Nile Virus (WNV) in horses inhabiting the Black Sea Region of Turkey using real time RT-PCR, a nucleic acid amplification test.
- In pursuit of this objective, the researchers collected serum samples from 120 horses equally from four different provinces in the Middle Black Sea region.
- These samples were then examined for the presence of WNV using Taqman based real time RT-PCR, a quantitative technique that allows the direct detection and quantification of specific nucleic acids in a sample.
Research Findings and Conclusion
- The researchers found that none of the horse serum samples contained nucleic acid related to West Nile Virus.
- The studies found no evidence of WNV-RNA in horses from the Black Sea Region, despite the region’s suitable conditions for WNV infection.
- Considering that the West Nile Virus, transmitted primarily by mosquitos, can cause neurologic disorders in horses and humans, the absence of WNV nucleic acid in these samples is a valuable finding.’
Future Research and Applications
- Despite their initial findings, the researchers maintain that it is necessary to continue their investigation due to the potential for infection in the region.
- Further research will focus on identifying the virus in vectors (organisms that do not cause disease but transmit infection by conveying pathogens from one host to another) and susceptible animals like dogs, besides horses.
- By better understanding the transmission and prevalence of this virus in different regions, future proactive measures and strategies can be developed to prevent and control the spread of the virus.
Cite This Article
APA
Yazici Z, Albayrak H, Ozan E, Gumusova S.
(2012).
The first investigation of west nile virus in horses using real time rt-PCR in middle black sea region in Turkey.
J Arthropod Borne Dis, 6(2), 151-155.
Publication
Researcher Affiliations
- Department of Virology, Faculty of Veterinary Medicine,Ondokuz Mayis University, Samsun, Turkey.
References
This article includes 27 references
- Apperson CS, Hassan HK, Harrison BA, Savage HM, Aspen SE, Farajollahi A, Crans W, Daniels TJ, Falco RC, Benedict M, Anderson M, McMillen L, Unnasch TR. Host feeding patterns of established and potential mosquito vectors of West Nile virus in the eastern United States.. Vector Borne Zoonotic Dis 2004 Spring;4(1):71-82.
- Ari A. [The prevalence and ecology of arboviruses in Turkey].. Turk Hij Tecr Biyol Derg (1961) 1972;32(2):134-43.
- Arpaci F, Cetin T, Kubar A, Ozturk M, Kuzhan O, Komurrcu S, Ozturk B, Ataergin S, Ozet A. West Nile infection in a patient with acute graft versus- host diseases.. Haemotologica 2009;94(Suppl 2):687.
- Blitvich BJ, Fernández-Salas I, Contreras-Cordero JF, Loroño-Pino MA, Marlenee NL, Díaz FJ, González-Rojas JI, Obregón-Martínez N, Chiu-García JA, Black WC 4th, Beaty BJ. Phylogenetic analysis of West Nile virus, Nuevo Leon State, Mexico.. Emerg Infect Dis 2004 Jul;10(7):1314-7.
- Bunning ML, Bowen RA, Cropp CB, Sullivan KG, Davis BS, Komar N, Godsey MS, Baker D, Hettler DL, Holmes DA, Biggerstaff BJ, Mitchell CJ. Experimental infection of horses with West Nile virus.. Emerg Infect Dis 2002 Apr;8(4):380-6.
- Cantile C, Del Piero F, Di Guardo G, Arispici M. Pathologic and immunohistochemical findings in naturally occuring West Nile virus infection in horses.. Vet Pathol 2001 Jul;38(4):414-21.
- Castillo-Olivares J, Wood J. West Nile virus infection of horses.. Vet Res 2004 Jul-Aug;35(4):467-83.
- Hayes EB, Komar N, Nasci RS, Montgomery SP, O'Leary DR, Campbell GL. Epidemiology and transmission dynamics of West Nile virus disease.. Emerg Infect Dis 2005 Aug;11(8):1167-73.
- Hubálek Z, Halouzka J. West Nile fever--a reemerging mosquito-borne viral disease in Europe.. Emerg Infect Dis 1999 Sep-Oct;5(5):643-50.
- Katsarov G, Vasilenko S, Vargin V, Butenko S, Tkachenko E. Serological studies on the distribution of some arboviruses in Bulgaria.. Problems of Infec and Parasitic Dis 1980;8:32–35.
- Komar N. West Nile viral encephalitis.. Rev Sci Tech 2000 Apr;19(1):166-76.
- Koptopoulos G, Papadopoulos O. A serological survey for tick-borne encephalitis and West Nile viruses in Greece.. Zentralbl Bakteriol (Suppl) 1980;9:185–188.
- Kramer LD, Bernard KA. West Nile virus in the western hemisphere.. Curr Opin Infect Dis 2001 Oct;14(5):519-25.
- Lanciotti RS, Kerst AJ, Nasci RS, Godsey MS, Mitchell CJ, Savage HM, Komar N, Panella NA, Allen BC, Volpe KE, Davis BS, Roehrig JT. Rapid detection of west nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay.. J Clin Microbiol 2000 Nov;38(11):4066-71.
- Meço O. [West Nile arbovirus antibodies with hemagglutination inhibition (HI) in residents of Southeast Anatolia].. Mikrobiyol Bul 1977 Jan;11(1):3-17.
- Monath TP, Heinz FX. Flaviviruses.. Virology 1996. pp. 961–1035.
- Murgue B, Murri S, Zientara S, Durand B, Durand JP, Zeller H. West Nile outbreak in horses in southern France, 2000: the return after 35 years.. Emerg Infect Dis 2001 Jul-Aug;7(4):692-6.
- Ostlund EN, Crom RL, Pedersen DD, Johnson DJ, Williams WO, Schmitt BJ. Equine West Nile encephalitis, United States.. Emerg Infect Dis 2001 Jul-Aug;7(4):665-9.
- Ozkul A, Yildirim Y, Pinar D, Akcali A, Yilmaz V, Colak D. Serological evidence of West Nile Virus (WNV) in mammalian species in Turkey.. Epidemiol Infect 2006 Aug;134(4):826-9.
- Ozer N, Ergünay K, Simsek F, Kaynas S, Alten B, Caglar SS, Ustacelebi S. West Nile virus studies in the Sanliurfa Province of Turkey.. J Vector Ecol 2007 Dec;32(2):202-6.
- Petersen LR, Roehrig JT. West Nile virus: a reemerging global pathogen.. Emerg Infect Dis 2001 Jul-Aug;7(4):611-4.
- Porter KR, Summers PL, Dubois D, Puri B, Nelson W, Henchal E, Oprandy JJ, Hayes CG. Detection of West Nile virus by the polymerase chain reaction and analysis of nucleotide sequence variation.. Am J Trop Med Hyg 1993 Mar;48(3):440-6.
- Radda A. [Studies on the activity and ecology of arboviruses in Turkey (author's transl)].. Zentralbl Bakteriol Orig A 1973 Oct;225(1):19-26.
- Rappole JH, Derrickson SR, Hubálek Z. Migratory birds and spread of West Nile virus in the Western Hemisphere.. Emerg Infect Dis 2000 Jul-Aug;6(4):319-28.
- Savage HM, Ceianu C, Nicolescu G, Karabatsos N, Lanciotti R, Vladimirescu A, Laiv L, Ungureanu A, Romanca C, Tsai TF. Entomologic and avian investigations of an epidemic of West Nile fever in Romania in 1996, with serologic and molecular characterization of a virus isolate from mosquitoes.. Am J Trop Med Hyg 1999 Oct;61(4):600-11.
- Tber Abdelhaq A. West Nile fever in horses in Morocco.. Bull de l’Office International des Epizooties 1996;11:867–879.
- Zeller HG, Schuffenecker I. West Nile virus: an overview of its spread in Europe and the Mediterranean basin in contrast to its spread in the Americas.. Eur J Clin Microbiol Infect Dis 2004 Mar;23(3):147-56.
Citations
This article has been cited 3 times.- Albayrak H, Sahindokuyucu I, Muftuoglu B, Tamer C, Kadi H, Ozan E, Yilmaz O, Kilic H, Kurucay HN, Coven F, Gumusova S, Yazici Z, Elhag AE. Sentinel serosurveillance of backyard hens proved West Nile virus circulation in the western provinces of Turkey. Vet Med Sci 2021 Nov;7(6):2348-2352.
- Staji H, Keyvanlou M, Geraili Z, Shahsavari H, Jafari E. The First Study of West Nile Virus in Feral Pigeons (Columba livia domestica) Using Conventional Reverse Transcriptase PCR in Semnan and Khorasane-Razavi Provinces, Northeast of Iran. J Arthropod Borne Dis 2021 Mar;15(1):136-142.
- Ozan E, Albayrak H, Gumusova S, Bolukbas CS, Kurt M, Pekmezci GZ, Beyhan YE, Kadi H, Kaya S, Aydin I, Yazici Z. A Study on the Identification of Five Arboviruses from Hematophagous Mosquitoes and Midges Captured in Some Parts of Northern Turkey. J Arthropod Borne Dis 2019 Jun;13(2):224-233.
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