The Impact of Reproductive Technologies on Stallion Mitochondrial Function.
Abstract: The traditional assessment of stallion sperm comprises evaluation of sperm motility and membrane integrity and identification of abnormal morphology of the spermatozoa. More recently, the progressive introduction of flow cytometry is increasing the number of tests available. However, compared with other sperm structures and functions, the evaluation of mitochondria has received less attention in stallion andrology. Recent research indicates that sperm mitochondria are key structures in sperm function suffering major changes during biotechnological procedures such as cryopreservation. In this paper, mitochondrial structure and function will be reviewed in the stallion, when possible specific stallion studies will be discussed, and general findings on mammalian mitochondrial function will be argued when relevant. Especial emphasis will be put on their role as source of reactive oxygen species and in their role regulating sperm lifespan, a possible target to investigate with the aim to improve the quality of frozen-thawed stallion sperm. Later on, the impact of current sperm technologies, principally cryopreservation, on mitochondrial function will be discussed pointing out novel areas of research interest with high potential to improve current sperm technologies.
© 2015 Blackwell Verlag GmbH.
Publication Date: 2015-06-01 PubMed ID: 26031351DOI: 10.1111/rda.12551Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Review
Summary
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The researchers in this study aimed to understand how reproductive technologies, particularly cryopreservation, influence the structure and function of mitochondria in stallion sperm. They also examined the role of mitochondria as a source of reactive oxygen species and in regulating sperm lifespan.
Introduction and Objective:
- This research focuses on understanding the impact of reproductive technologies, such as cryopreservation, on the mitochondria of stallion sperm.
- The research also delves into the role of mitochondria as a source of reactive oxygen species and as a regulator of sperm lifespan.
Research Context:
- The traditional methods of evaluating stallion sperm rely on the assessment of sperm motility, membrane integrity, and identification of abnormal sperm morphology.
- Recently, flow cytometry has opened up the possibility to conduct more tests. However, examination of mitochondria, which is a crucial factor in sperm functionality, has been primarily neglected in stallion andrology studies.
Research Scope:
- The researchers in this paper specifically review the structure and function of the mitochondria in stallion sperm.
- They discuss every possible stallion study in particular, and argue about the general findings relevant to mammalian mitochondrial function.
Findings and Suggestions:
- Initial findings indicate that sperm mitochondria undergo significant changes during biotechnological processes, especially in cryopreservation.
- The researchers emphasize the role of mitochondria as a source of reactive oxygen species and in regulating sperm lifespan.
- The research proposes that these areas may be potential targets for further investigation to improve the quality of frozen-thawed stallion sperm.
- The impacts of current sperm technologies, specifically cryopreservation, on mitochondrial function are discussed, revealing new areas of research interest that could lead to improvements in current sperm technologies.
Impact:
- The findings from this research can play a significant role in enhancing reproductive technologies by understanding the core mitochondrial functions that influence the quality and lifespan of sperm.
Cite This Article
APA
Peña FJ, Plaza Davila M, Ball BA, Squires EL, Martin Muñoz P, Ortega Ferrusola C, Balao da Silva C.
(2015).
The Impact of Reproductive Technologies on Stallion Mitochondrial Function.
Reprod Domest Anim, 50(4), 529-537.
https://doi.org/10.1111/rda.12551 Publication
Researcher Affiliations
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Gluck Equine Research Center, University of Kentucky, Lexington, KY, USA.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Gluck Equine Research Center, University of Kentucky, Lexington, KY, USA.
- Gluck Equine Research Center, University of Kentucky, Lexington, KY, USA.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
MeSH Terms
- Animals
- Apoptosis
- Cell Separation
- Cell Survival
- Cryopreservation / veterinary
- Fertilization
- Horses
- Male
- Mitochondria / physiology
- Mitochondria / ultrastructure
- Osmolar Concentration
- Oxidative Stress
- Reactive Oxygen Species
- Reproductive Techniques / veterinary
- Semen Preservation / adverse effects
- Semen Preservation / methods
- Semen Preservation / veterinary
- Sex Preselection / methods
- Spermatozoa / physiology
- Spermatozoa / ultrastructure
Citations
This article has been cited 15 times.- Bucci D, Spinaci M, Bustamante-Filho IC, Nesci S. The sperm mitochondria: clues and challenges. Anim Reprod 2022;19(4):e20220131.
- Asadi E, Najafi A, Benson JD. Exogenous Melatonin Ameliorates the Negative Effect of Osmotic Stress in Human and Bovine Ovarian Stromal Cells. Antioxidants (Basel) 2022 May 26;11(6).
- Qin Z, Wang W, Ali MA, Wang Y, Zhang Y, Zhang M, Zhou G, Yang JD, Zeng C. Transcriptome-wide m(6)A profiling reveals mRNA post-transcriptional modification of boar sperm during cryopreservation. BMC Genomics 2021 Aug 3;22(1):588.
- Lançoni R, Celeghini ECC, Giuli V, de Carvalho CPT, Zoca GB, Garcia-Oliveros LN, Batissaco L, Oliveira LZ, de Arruda RP. Coenzyme Q-10 improves preservation of mitochondrial functionality and actin structure of cryopreserved stallion sperm. Anim Reprod 2021 Mar 31;18(1):e20200218.
- Bucci D, Spinaci M, Galeati G, Tamanini C. Different approaches for assessing sperm function. Anim Reprod 2020 May 22;16(1):72-80.
- Shan D, Arhin SK, Zhao J, Xi H, Zhang F, Zhu C, Hu Y. Effects of SLIRP on Sperm Motility and Oxidative Stress. Biomed Res Int 2020;2020:9060356.
- Peña FJ, O'Flaherty C, Ortiz Rodríguez JM, Martín Cano FE, Gaitskell-Phillips GL, Gil MC, Ortega Ferrusola C. Redox Regulation and Oxidative Stress: The Particular Case of the Stallion Spermatozoa. Antioxidants (Basel) 2019 Nov 19;8(11).
- Ortiz-Rodriguez JM, Balao da Silva C, Masot J, Redondo E, Gazquez A, Tapia JA, Gil C, Ortega-Ferrusola C, Peña FJ. Rosiglitazone in the thawing medium improves mitochondrial function in stallion spermatozoa through regulating Akt phosphorylation and reduction of caspase 3. PLoS One 2019;14(7):e0211994.
- Ortiz-Rodriguez JM, Ortega-Ferrusola C, Gil MC, Martín-Cano FE, Gaitskell-Phillips G, Rodríguez-Martínez H, Hinrichs K, Álvarez-Barrientos A, Román Á, Peña FJ. Transcriptome analysis reveals that fertilization with cryopreserved sperm downregulates genes relevant for early embryo development in the horse. PLoS One 2019;14(6):e0213420.
- Dai DH, Qazi IH, Ran MX, Liang K, Zhang Y, Zhang M, Zhou GB, Angel C, Zeng CJ. Exploration of miRNA and mRNA Profiles in Fresh and Frozen-Thawed Boar Sperm by Transcriptome and Small RNA Sequencing. Int J Mol Sci 2019 Feb 13;20(4).
- Liu Q, Lei Z, Huang A, Lu Q, Wang X, Ahmed S, Awais I, Yuan Z. Mechanisms of the Testis Toxicity Induced by Chronic Exposure to Mequindox. Front Pharmacol 2017;8:679.
- Ortiz-Rodriguez JM, Anel-Lopez L, Martín-Muñoz P, Álvarez M, Gaitskell-Phillips G, Anel L, Rodríguez-Medina P, Peña FJ, Ortega Ferrusola C. Pulse Doppler ultrasound as a tool for the diagnosis of chronic testicular dysfunction in stallions. PLoS One 2017;12(5):e0175878.
- Plaza Davila M, Martin Muñoz P, Tapia JA, Ortega Ferrusola C, Balao da Silva C C, Peña FJ. Inhibition of Mitochondrial Complex I Leads to Decreased Motility and Membrane Integrity Related to Increased Hydrogen Peroxide and Reduced ATP Production, while the Inhibition of Glycolysis Has Less Impact on Sperm Motility. PLoS One 2015;10(9):e0138777.
- Wang R, Adetunji AO, Min L, Zhu Z. Mitochonic acid 5 increases boar sperm quality by mitigating mitochondrial dysfunction. Front Cell Dev Biol 2025;13:1583951.
- Peña FJ, Martín-Cano FE, Becerro-Rey L, da Silva-Álvarez E, Gaitskell-Phillips G, Aparicio IM, Gil MC, Ortega-Ferrusola C. Redox Regulation and Glucose Metabolism in the Stallion Spermatozoa. Antioxidants (Basel) 2025 Feb 17;14(2).
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