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Home / Equine Research Bank / Animals (Basel) / The Indicators of Clinical and Subclinical Mastitis in Equin...
Animals : an open access journal from MDPI2022; 12(4); doi: 10.3390/ani12040440

The Indicators of Clinical and Subclinical Mastitis in Equine Milk.

Abstract: The somatic cell count in milk (SCC) and electrical conductivity of milk (ECM) are indicators of the health status of the mammary gland. Among somatic cells, mainly polymorphonuclear neutrophils (PMN), macrophages (MAC), and lymphocytes (LYM) are rated. This study aimed to determine the SCC, PMN, MAC, LYM, ECM, and bacteriological index (BII) in milk collected from mares with (CM) and without (NCM) clinical symptoms of mastitis concerning mares with (SM) and without (NSM) subclinical mastitis. Milk samples were collected from 27 mares divided into NCM (n = 12) and CM (n = 15) groups. In samples, SCC quantification, cytological examinations, ECM measurement, and bacteriological examination were performed. In NCM mares, the values of SCC, PMN, MAC, LYM, and ECM were higher in initial than in consecutive examined days after birth until weaning. After weaning the proportion of SCC, PMN, MAC, LYM, ECM, and BII increased and did not differ with the average values in CM mares. These equine milk indicators may reflect an early symptom of subclinical mastitis and in the future may be used in the early detection of mastitis or as a tool of assessment of the health status of the mammary gland in the dairy equine farm.
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Publication Date: 2022-02-11 PubMed ID: 35203147PubMed Central: PMC8868146DOI: 10.3390/ani12040440Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on the identification of indicators for clinical and subclinical mastitis in equine milk. Specifically, it examines the changes in somatic cell count, certain cell types, electrical conductivity, and bacteriological indexes in the milk of mares with varying health states.

Study Design and Objectives

  • The study aimed at determining the somatic cell count (SCC), types of somatic cells in milk (polymorphonuclear neutrophils or PMN, macrophages or MAC, lymphocytes or LYM), electrical conductivity of milk (ECM), and bacteriological index (BII) in milk gathered from horses with (CM) and without (NCM) clinical mastitis symptoms, and if they had (SM) or did not have (NSM) subclinical mastitis.
  • It aimed to identify any changes in these parameters as potential indicators of early signs of subclinical mastitis that could be used for assessment of dairy horses’ mammary gland health.

Methodology

  • Milk samples were collected from a total of 27 mares divided into the NCM (n=12) and CM (n=15) groups.
  • The samples underwent SCC quantification, cytological examinations, ECM measurement, and bacteriological examination.

Findings and Conclusions

  • In horses without clinical mastitis symptoms (NCM), the values for SCC, PMN, MAC, LYM, and ECM were found to be higher in initial days after birth compared to consecutive days until weaning.
  • However, after weaning, the proportion of SCC, PMN, MAC, LYM, ECM, and BII increased and were found not to be significantly different from the average values found in horses with clinical mastitis symptoms (CM).
  • The researchers concluded that these changes in equine milk could indicate early symptoms of subclinical mastitis.
  • The results of this study could potentially contribute in the development of effective screening tools for early detection of mastitis or in assessing the health status of the mammary gland in the horse dairy industry.

Cite This Article

APA
Domańska D, Trela M, Pawliński B, Podeszewski B, Domino M. (2022). The Indicators of Clinical and Subclinical Mastitis in Equine Milk. Animals (Basel), 12(4). https://doi.org/10.3390/ani12040440

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 12
Issue: 4

Researcher Affiliations

Domańska, Dominika
  • Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
Trela, Michał
  • Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
Pawliński, Bartosz
  • Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
Podeszewski, Bartłomiej
  • Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
Domino, Małgorzata
  • Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 63 references
  1. Brumini D, Criscione A, Bordonaro S, Vegarud G.E., Marletta D. Whey proteins and their antimicrobial properties in donkey milk: A brief review.. Dairy Sci. Technol. 2016;96:1–14.
    doi: 10.1007/s13594-015-0246-1google scholar: lookup
  2. Fotschki J, Szyc A.M., Laparra J.M., Markiewicz L.H., Wróblewska B. Immune-modulating properties of horse milk administered to mice sensitized to cow milk.. J. Dairy Sci. 2016;99:9395–9404.
    doi: 10.3168/jds.2016-11499pubmed: 27771084google scholar: lookup
  3. Kushugulova A, Kozhakhmetov S, Sattybayeva R, Nurgozhina A, Ziyat A, Yadav H, Marotta F. Mare’s milk as a prospective functional product.. Funct. Food Health Dis. 2018;8:537–543.
    doi: 10.31989/ffhd.v8i11.528google scholar: lookup
  4. Aspri M, Leni G, Galaverna G, Papademas P. Bioactive properties of fermented donkey milk, before and after in vitro simulated gastrointestinal digestion.. Food Chem. 2018;268:476–484.
    doi: 10.1016/j.foodchem.2018.06.119pubmed: 30064786google scholar: lookup
  5. Miraglia N, Salimei E, Fantuz F. Equine milk production and valorization of marginal areas—A review.. Animals 2020;10:353.
    doi: 10.3390/ani10020353pmc: PMC7070972pubmed: 32098374google scholar: lookup
  6. Drogoul C, Prevost H, Maubois J.L. Quoi de Neuf en Matiere d’Etudes de Recherches sur le Cheval. 18eme Journee d’Etude. 4 Mars. CEREOPA; Paris, France: 1992. Le lait de juments un produit. Une filiere a developer?. pp. 37–51.
  7. Doreau M, Martin-Rosset W. Animals that produce dairy foods—horse.. Encyclopedia of Dairy Sciences 2nd ed. Volume 1. Elsevier Academy Press; London, UK: 2011. pp. 358–364.
  8. Salimei E, Fantuz F. Horse and donkey milk.. Milk and Dairy Products in Human Nutrition: Production. Composition and Health John Wiley & Sons Ltd.; Oxford, UK: 2013. pp. 594–613.
  9. McLean A.K., Navas Gonzalez F.J. Can scientists influence donkey welfare? Historical perspective and a contemporary view.. J. Equine Vet. Sci. 2018;65:25–32.
    doi: 10.1016/j.jevs.2018.03.008google scholar: lookup
  10. Businco L, Giampietro P.G., Lucenti P, Lucaroni F, Pini C, Di Felice G, Iacovacci P, Curadi C, Orlandi M. Allergenicity of mare’s milk in children with cow’s milk allergy.. J. Allergy Clin. Immunol. 2000;105:1031–1034.
    doi: 10.1067/mai.2000.106377pubmed: 10808187google scholar: lookup
  11. Sarti L, Martini M, Brajon G, Barni S, Salari F, Altomonte I, Ragona G, Mori F, Pucci N, Muscas G. Donkey’s milk in the management of children with cow’s milk protein allergy: Nutritional and hygienic aspects.. Ital. J. Pediatrics 2019;45:102–110.
    doi: 10.1186/s13052-019-0700-4pmc: PMC6698021pubmed: 31420060google scholar: lookup
  12. Stuparu A.A., Strugariu C.E., Oroian T. Pharmaceuticals and Dietary Supplements Extracted from Mare’s Milk.. Bul. Univ. Agric. Sci. Vet. Med. Cluj-Napoca. Anim. Sci. Biotechnol. 2016;73:1–10.
    doi: 10.15835/buasvmcn-asb:11429google scholar: lookup
  13. Uniacke-Lowe T, Huppertz T, Fox P.F. Equine milk proteins: Chemistry, structure and nutritional significance.. Int. Dairy J. 2010;20:609–629.
    doi: 10.1016/j.idairyj.2010.02.007google scholar: lookup
  14. Pastuszka R, Barłowska J, Litwińczuk Z. Allergenicity of milk of different animal species in relation to human milk.. Postepy Hig. I Med. Dosw. 2016;70:1451–1459.
    doi: 10.5604/17322693.1227842pubmed: 28100852google scholar: lookup
  15. Fantuz F, Salimei E, Papademas P. Macro- and micronutrients in non-cow milk and products and their impact on human health.. Non-Bovine Milk and Milk Products 1st ed. Elsevier Academic Press; London, UK: 2016. pp. 209–261.
  16. Ballard O, Morrow A.L. Human milk composition: Nutrients and bioactive factors.. Pediatr. Clin. N. Am. 2013;60:49–74.
    doi: 10.1016/j.pcl.2012.10.002pmc: PMC3586783pubmed: 23178060google scholar: lookup
  17. Bonnet M, Delavaud C, Laud K, Gourdou I, Leroux C, Djiane J, Chilliard Y. Mammary leptin synthesis, milk leptin and their putative physiological roles.. Reprod. Nutr. Dev. 2002;42:399–413.
    doi: 10.1051/rnd:2002034pubmed: 12537253google scholar: lookup
  18. Inglingstad R.A., Devold T.G., Eriksen E.K., Holm H, Jacobsen M, Liland K.H., Rukke E.O., Vegarud G.E. Comparison of the digestion of caseins and whey proteins in equine, bovine, caprine and human milk by human gastrointestinal enzymes.. Dairy Sci. Technol. 2010;90:549–563.
    doi: 10.1051/dst/2010018google scholar: lookup
  19. Morand-Fehr P, Fedele V, Decandia M, Le Frileux Y. Influence of farming and feeding systems on composition and quality of goat and sheep milk.. Small Rumin. Res. 2007;68:20–34.
    doi: 10.1016/j.smallrumres.2006.09.019google scholar: lookup
  20. Mostafa T.H., El-Malky O.M., Abd El-Salaam A.M., Nabih A.M. Some Studies on Milk Production and its Composition In Maghrebi She-Camel Farming And Traditional Pastoral Systems In Egypt.. Int. J. Hortic. Agric. 2017;2:1–9.
  21. Vicini J, Etherton T, Kris-Etherton P, Ballam J, Denham S, Staub R, Goldstein D, Cady R, McGrath M, Lucy M. Survey of retail milk composition as affected by label claims regarding farm-management practices.. J. Am. Diet. Assoc. 2008;108:1198–1203.
    doi: 10.1016/j.jada.2008.04.021pubmed: 18589029google scholar: lookup
  22. Li L, Liu X, Guo H. The nutritional ingredients and antioxidant activity of donkey milk and donkey milk powder.. Food Sci. Biotechnol. 2018;27:393–400.
    doi: 10.1007/s10068-017-0264-2pmc: PMC6049624pubmed: 30263762google scholar: lookup
  23. Kaic A, Luštrek B, Simcic M, Potocnik K. Milk quantity. composition and hygiene traits of routinely machine milked Lipizzan mares.. Slov. Vet. Res. 2019;56:115–123.
  24. Colavita G, Amadoro C, Rossi F, Fantuz F, Salimei E. Hygienic characteristics and microbiological hazard identification in horse and donkey raw milk.. Vet Ital. 2016;52:21–29.
    pubmed: 27033528
  25. Cieslak J, Mackowski M, Czyzak-Runowska G, Wojtowski J, Puppel K, Kuczynska B, Pawlak P. Screening for the most suitable reference genes for gene expression studies in equine milk somatic cells.. PLoS ONE 2015;10:e0139688.
    doi: 10.1371/journal.pone.0139688pmc: PMC4593561pubmed: 26437076google scholar: lookup
  26. Canisso I.F., Podico G, Ellerbrock R.E. Diagnosis and treatment of mastitis in mares.. Equine Vet. Educ. 2021;33:320–326.
    doi: 10.1111/eve.13228google scholar: lookup
  27. Hughes K. Development and Pathology of the Equine Mammary Gland.. J. Mammary Gland Biol. Neoplasia. 2021;26:121–134.
    doi: 10.1007/s10911-020-09471-2pmc: PMC8236023pubmed: 33280071google scholar: lookup
  28. Birhanu M, Leta S, Mamo G, Tesfaye S. Prevalence of bovine subclinical mastitis and isolation of its major causes in Bishoftu Town. Ethiopia.. BMC Res. Notes 2017;10:767.
    doi: 10.1186/s13104-017-3100-0pmc: PMC5740909pubmed: 29268785google scholar: lookup
  29. Chakraborty S, Dhama K, Tiwari R, Iqbal Yatoo M, Khurana S.K., Khandia R, Munja A, Munuswamy P, Kumar M.A., Singh M. Technological interventions and advances in the diagnosis of intramammary infections in animals with emphasis on bovine population—A review.. Vet. Q. 2019;39:76–94.
    doi: 10.1080/01652176.2019.1642546pmc: PMC6830988pubmed: 31288621google scholar: lookup
  30. Perkins N.R., Threlfall W.R. Mastitis in the mare.. Equine Vet. Educ. 2002;5:99–102.
    doi: 10.1111/j.2042-3292.2002.tb01804.xgoogle scholar: lookup
  31. Murata H, Shimada N, Yoshioka M. Current research on acute phase proteins in veterinary diagnosis: An overview.. Vet. J. 2004;168:28–40.
    doi: 10.1016/S1090-0233(03)00119-9pubmed: 15158206google scholar: lookup
  32. McCue P.M., Wilson W.D. Equine mastitis—A review of 28 cases.. Equine Vet. J. 1989;21:351–353.
    doi: 10.1111/j.2042-3306.1989.tb02687.xpubmed: 2776721google scholar: lookup
  33. Rhaymah M, Al-Obaidi Q.T., Hamad M.A., Altalby M.A. Mastitis in mare: Case report.. Iraqi J. Vet. Sci. 2018;32:109–111.
  34. Böhm K.H., Klug E, Jacobs B.J. Mastitis in the mare-a long-term study on the incidence, clinical symptoms, diagnostics, microbiology, therapy and economic importance. as well as recommendations for veterinary practice.. Prakt. Tierarzt. 2009;90:842–849.
  35. Motta R.G., Listoni F.J.P., Ribeiro M.G., Bussalaro V.A., Link A, Almeida R.P., Cappellozza B.I. Microbiologic characterization of equine mastitis.. J. Bacteriol. Parasitol. 2014;5:186–188.
  36. Kitchen B.J. Review of the progress of dairy science: Bovine mastitis: Milk compositional changes and related diagnostic tests.. J. Dairy Sci. 1981;64:167–188.
    pubmed: 7021617
  37. Kamphuis C, Pietersma D, Van der Tol R, Wiedemann M, Hogeveen H. Using sensor data patterns from an automatic milking system to develop predictive variables for classifying clinical mastitis and abnormal milk.. Comput. Electron. Agric. 2008;62:169–181.
    doi: 10.1016/j.compag.2007.12.009google scholar: lookup
  38. Prescott S.C., Breed R.S. The determination of the number of body cells in milk by a direct method.. J. Infect. Dis. 1910;7:632–640.
    doi: 10.1093/infdis/7.5.632pmc: PMC2543863pubmed: 19599514google scholar: lookup
  39. Kulisa M, Makieła K, Hofman J. Zawartość komórek somatycznych w mleku klaczy a rasa, wiek, kolejność i miesiąc laktacji.. Rocz. Nauk. Zootech. 2010;37:131–135.
  40. Schröder A. Untersuchung zum Zelldifferentialbild in Milch und Blut unter Berücksichtigung des Gesundheitsstatus der bovinen Milchdrüse.. Ph.D. Thesis. University of Veterinary Medicine; Hannover, Germany: 2003 .
  41. Danków R, Wójtowski J, Pikul J, Niżnikowski R, Cais-Sokolińska D. Effect of lactation on the hygiene quality and some milk physicochemical traits of the Wielkopolska mares.. Arch. Tierzucht. Dummerstorf. 2006;49:201–206.
  42. Danków R, Pikul J, Osten-Sacken N, Teichert J. Charakterystyka i właściwości prozdrowotne mleka klaczy.. Nauka Przyr. Technol. 2012;6:2–16.
  43. Dohoo I, Martin W, Stryhn H. Veterinary Epidemiologic Research.. 2nd ed. VER Inc.; Charlottetown, PE, Canada: 2009.
  44. Oftedal O.T., Dhouailly D. Evo-devo of the mammary gland.. J. Mammary Gland Biol. Neoplasia. 2013;18:105–120.
    doi: 10.1007/s10911-013-9290-8pubmed: 23681303google scholar: lookup
  45. Oftedal O.T. The evolution of lactation in mammalian species.. Milk. Mucosal Immun. Microbiome Impact Neonate. 2020;94:1–10.
    pubmed: 32155641
  46. Oviedo-Boyso J, Valdez-Alarcon J.J., Cajero-Juarez M, Ochoa-Zarzosa A, Lopez-Meza J.E., Bravo-Patino A, Baizabal-Aguirre M. Innate immune response of bovine mammary gland to pathogenic bacteria responsible for mastitis.. J. Infect. 2007;54:399–409.
    doi: 10.1016/j.jinf.2006.06.010pubmed: 16882453google scholar: lookup
  47. Rieland E. Untersuchungen zu den Aktivitäten der Enzyme Lysosym, LDH, Gamma—GT, GOT, GTP. Laktoperoxydase und Bestimmung der Zellzahl in Stutenmilch im Laktationsverlauf. Ph.D. Thesis. Justus Liebig-Universität; Giessen, Germany: 1997 pp. 1–98.
  48. Markiewicz-Kęszycka M. Wartość Odżywcza Oraz Prozdrowotna Siary i Mleka Klaczy w Diecie Człowieka. Ph.D. Thesis. Uniwersytet Przyrodniczy w Poznaniu; Poznań, Poland: 2012 pp. 3–106.
  49. Kuy G. Untersuchungen zur Qualität von Frischer und Tiefgefriergetrockneter Stutenmilch. Ph.D. Thesis. Universität Leipzig; Leipzig, Germany: 1998 pp. 1–103.
  50. Pecka E, Dobrzański Z, Zachwieja A, Szulc T, Czyż K. Studies of composition and major protein level in milk and colostrum of mares.. Anim. Sci. J. 2012;83:162–168.
    doi: 10.1111/j.1740-0929.2011.00930.xpubmed: 22339698google scholar: lookup
  51. Kehrli M.E. Jr., Shuster D.E. Factors affecting milk somatic cells and their role in health of the bovine mammary gland.. J. Dairy Sci. 1994;77:619–627.
    doi: 10.3168/jds.S0022-0302(94)76992-7pubmed: 8182187google scholar: lookup
  52. Sharma N, Singh N.K., Bhadwal M.S. Relationship of somatic cell count and mastitis: An overview.. Asian-Australas J. Anim. Sci. 2011;24:429–438.
    doi: 10.5713/ajas.2011.10233google scholar: lookup
  53. Litwińczuk Z, Król J, Brodziak A, Barłowska J. Changes of protein content and its fractions in bovine milk from different breeds subject to somatic cell count.. J. Dairy Sci. 2011;94:684–691.
    doi: 10.3168/jds.2010-3217pubmed: 21257037google scholar: lookup
  54. Prestes N.C., Langoni H, Cordeiro L.A.V. Study of the milk in healthy mares or mares with subclinics mastitis using Whiteside test. microbiological culture and somatic cell count.. Braz. J. Vet. Res. Anm. Sci. 1999;36:144–148.
  55. Wendt K, Bostedt H, Mielke H, Fuchs W. Euter- und Gesäugekrankheiten.. Fischer Verlag; Stuttgart, Germany: 1994 .
  56. Jackson P.G.G. Equine mastitis: Comparative lessons.. Equine Vet. J. 1986;18:88–89.
    doi: 10.1111/j.2042-3306.1986.tb03550.xpubmed: 3698961google scholar: lookup
  57. Bostedt H, Lehmann B, Peip D. Zur Problematik der Mastitis bei Stuten.. Tierärztl. Prax. 1988;16:367–371.
    pubmed: 3065979
  58. Oshima M. Detection of abnormal quarter milk by quarter difference of electrical conductivity and its theoretical basis.. Jpn. Agric. Res. Q. 1977;11:239–245.
  59. Sordillo L.M., Streicher K.L. Mammary gland immunity and mastitis susceptibility.. J. Mammary Gland Biol. Neopla. 2002;7:135–146.
    doi: 10.1023/A:1020347818725pubmed: 12463736google scholar: lookup
  60. Salimei E, Park Y.W. Mare milk.. Handbook of Milk of Non-Bovine Mammals 2nd ed. John Wiley & Sons Ltd.; Oxford, UK: 2017. pp. 369–408.
  61. Ying G.S., Maguire M.G., Glynn R.J., Rosner B. Calculating sensitivity, specificity, and predictive values for correlated eye data.. IOVS 2020;61:29.
    doi: 10.1167/iovs.61.11.29pmc: PMC7500131pubmed: 32936302google scholar: lookup
  62. Sumon S.M.R., Parvin M.S., Ehsan M.A., Islam M.T. Relationship between somatic cell counts and subclinical mastitis in lactating dairy cows.. Vet. World 2020;13:1709.
    doi: 10.14202/vetworld.2020.1709-1713pmc: PMC7522932pubmed: 33061248google scholar: lookup
  63. Norberg E, Hogeveen H, Korsgaard I.R., Friggens N.C., Sloth K.H.M.N., Løvendahl P. Electrical Conductivity of Milk: Ability to Predict Mastitis Status.. J. Dairy Sci. 2004;87:1099–1107.
    doi: 10.3168/jds.S0022-0302(04)73256-7pubmed: 15259246google scholar: lookup
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