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Journal of animal physiology and animal nutrition2020; 104(6); 1919-1926; doi: 10.1111/jpn.13325

The influence of a simulated digest of an equine dietary feed additive G’s formula on contractile activity of gastric smooth muscle in vitro.

Abstract: G's Formula is a novel equine feed additive formulated to promote optimal GI function. The objective of this study was to determine whether the addition of a simulated digest of the composite feed additive G's Formula (FA) would alter the contractile response of gastric smooth muscle to acetylcholine (Ach). Smooth muscle strips from porcine stomachs were excised and attached to an isometric force transducer. An experiment was run to compare tissue contraction between tissue exposed to FA (FA; n = 8, simulated digest of FA was added to the bath) and control tissue (CO; n = 8, no additions made). Increasing concentrations of Ach were added into the bath such that the concentration increased from 10 -10  M. Based on the analysis of these data, a difference between FA and CO was observed. Therefore, another trial was run which included a blank group (BL n = 6) in which the tissue was exposed to the simulated digest without FA. More CO (n = 5) and FA (n = 4) tissue was also run. Force was compared to baseline and between groups. In FA group, mean force for 1-min following all Ach additions was higher than baseline (p < .05) and by 2-min the integral-under-force/time curve (AUC) was higher than baseline from 10 -10  M compared to lower concentrations of Ach in both CO (10  M for both) and BL (10  M and 10  M, respectively). By 8-min AUC of all Ach concentrations were higher than baseline in FA compared to an Ach of 10  M in both CO and BL. A simulated digest of FA appears to sensitize gastric smooth muscle to Ach in vitro. FA may increase GI contractility, and the functional effect of this should be studied further in vivo.
© 2020 Blackwell Verlag GmbH.
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Publication Date: 2020-02-11 PubMed ID: 32048346DOI: 10.1111/jpn.13325Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the effect of equine dietary feed additive G’s Formula on the contraction of gastric smooth muscle against a biochemical substance acetylcholine. The study finds that the simulated digest of this feed additive may potentially increase gastrointestinal contractility, warranting further investigations in a live setting.

Experiment Setup

  • The aim of the study was to assess if a simulated digest of G’s Formula feed additive (FA) could alter the contraction of gastric smooth muscle when exposed to acetylcholine (Ach). Ach is a biochemical substance known to stimulate muscle contractions.
  • Smooth muscle strips from pig stomachs were used as the experimental model due to their similarity to horse stomach muscles. These muscles were attached to a device that measures force, the isometric force transducer.
  • Different trials were run where the muscle tissues were exposed to varying conditions:
  • FA group: Muscle tissue was exposed to the simulated digest of G’s Formula.
  • CO group: Control group where the muscle tissue was not exposed to any additives.
  • BL group: Blank group where the tissue was tested against the simulated digest without the feed additive.

Experiment Process and Findings

  • Different quantities of Ach were added to these various groups, starting from a molecular concentration of 10^-10 M.
  • Following each addition of Ach, the force exerted by the muscles was compared to baseline, and among the different groups.
  • It was found that the mean force generated by the muscles in the FA group a minute after all Ach additions was significantly higher than the baseline.
  • Within 2 minutes, the area under the force/time curve (AUC), a representation of the total contraction effect, was also found to be higher than the baseline for the FA group from 10^-10 M, compared to lower concentrations of Ach in both CO and BL groups.
  • By 8 minutes, the AUCs of all Ach concentrations were found to be higher within the FA group compared to Ach of 10^-10 M in both CO and BL groups.

Conclusion

  • The results of this study show that the simulated digest of G’s Formula feed additive appears to make the gastric smooth muscle more sensitive to Ach in a lab setting, leading to increased contractions.
  • This could imply that G’s Formula might increase gastrointestinal contractility in horses, thus aiding in digestion. However, the actual impact of this needs to be studied further in a real-life setting (in vivo).

Cite This Article

APA
MacNicol JL, Murrant C, Pearson W. (2020). The influence of a simulated digest of an equine dietary feed additive G’s formula on contractile activity of gastric smooth muscle in vitro. J Anim Physiol Anim Nutr (Berl), 104(6), 1919-1926. https://doi.org/10.1111/jpn.13325

Publication

Journal of animal physiology and animal nutrition
ISSN: 1439-0396
NlmUniqueID: 101126979
Country: Germany
Language: English
Volume: 104
Issue: 6
Pages: 1919-1926

Researcher Affiliations

MacNicol, Jennifer L
  • Department of Animal Biosciences, University of Guelph, Guelph, ON, Canada.
Murrant, Coral
  • Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada.
Pearson, Wendy
  • Department of Animal Biosciences, University of Guelph, Guelph, ON, Canada.

MeSH Terms

  • Acetylcholine
  • Animals
  • Horses
  • Muscle Contraction
  • Muscle, Smooth
  • Stomach
  • Swine

Grant Funding

  • Natural Sciences and Engineering Research Council of Canada
  • G's Organic Solutions Inc

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Citations

This article has been cited 2 times.
  1. MacNicol JL, Pearson W. Gastrin and Nitric Oxide Production in Cultured Gastric Antral Mucosa Are Altered in Response to a Gastric Digest of a Dietary Supplement. Front Vet Sci 2021;8:684203.
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  2. Prins A, Kosik O. Genetic Approaches to Increase Arabinoxylan and β-Glucan Content in Wheat. Plants (Basel) 2023 Sep 8;12(18).
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