The influence of environmental variables on platelet concentration in horse platelet-rich plasma.
Abstract: Platelet-rich plasma (PRP) commonly refers to blood products which contain a higher platelet (PLT) concentration as compared to normal plasma. Autologous PRP has been shown to be safe and effective in promoting the natural processes of soft tissue healing or reconstruction in humans and horses. Variability in PLT concentration has been observed in practice between PRP preparations from different patients or from the same individual under different conditions. A change in PLT concentration could modify PRP efficacy in routine applications. The aim of this study was to test the influence of environmental, individual and agonistic variables on the PLT concentration of PRP in horses. Six healthy Standardbred mares were exposed to six different variables with a one-week washout period between variables, and PRP was subsequently obtained from each horse. The variables were time of withdrawal during the day (morning/evening), hydration status (overhydration/dehydration) treatment with anti-inflammatory drugs and training periods on a treadmill. The platelet concentration was significantly higher in horses treated with a non-steroidal anti-inflammatory drug (P = 0.03). The leukocyte concentration increased 2-9 fold with respect to whole blood in the PRP which was obtained after exposure to all the variable considered. Environmental variation in platelet concentration should be taken into consideration during PRP preparation.
Publication Date: 2016-07-04 PubMed ID: 27377748PubMed Central: PMC4932754DOI: 10.1186/s13028-016-0226-3Google Scholar: Lookup
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- Journal Article
Summary
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This research article explores the factors affecting the concentration of platelets in horse platelet-rich plasma (PRP), a product typically used to speed up healing processes. The study concludes that factors such as time of blood withdrawal and hydration status can influence these platelet concentrations, affecting PRP efficacy in routine applications.
Objective of the Study
- The main aim of this study was to investigate the effects of different environmental factors, individual factors, and agonistic variables on the concentration of platelets in horses’ platelet-rich plasma.
Study Setup and Methodology
- For this research, six healthy Standardbred mares were used for the study.
- These horses were exposed to six different variables with a one-week gap (washout period) between each variable exposure. This was done to ensure that the effect of each variable was independently assessed.
- The variables considered in the study were: time of blood withdrawal (morning or evening), hydration status (overhydration or dehydration), treatment with anti-inflammatory drugs, and training periods on a treadmill.
- The researchers collected PRP from each horse after each variable exposure.
Findings of the Study
- It was found that platelet concentration significantly increased in horses that were treated with a non-steroidal anti-inflammatory drug.
- The researchers also noticed that the concentration of leukocytes (white blood cells) increased 2-9 times in the PRP obtained after horses were exposed to all the variables considered in this study.
Conclusions
- The results of the study suggest that there’s a significant variation in the concentration of platelets in horse PRP depending on different environmental conditions.
- The authors therefore suggest that these environmental variations should be taken into consideration during PRP preparation to maximize its efficacy in routine applications.
Cite This Article
APA
Rinnovati R, Romagnoli N, Gentilini F, Lambertini C, Spadari A.
(2016).
The influence of environmental variables on platelet concentration in horse platelet-rich plasma.
Acta Vet Scand, 58(1), 45.
https://doi.org/10.1186/s13028-016-0226-3 Publication
Researcher Affiliations
- Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano Dell'Emilia, BO, Italy.
- Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano Dell'Emilia, BO, Italy. noemi.romagnoli@unibo.it.
- Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano Dell'Emilia, BO, Italy.
- Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano Dell'Emilia, BO, Italy.
- Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano Dell'Emilia, BO, Italy.
MeSH Terms
- Animals
- Anti-Inflammatory Agents, Non-Steroidal / pharmacology
- Blood Platelets / cytology
- Blood Platelets / drug effects
- Dehydration / blood
- Dehydration / veterinary
- Environment
- Female
- Horses / blood
- Physical Conditioning, Animal
- Platelet Count / veterinary
- Platelet-Rich Plasma / cytology
- Time Factors
References
This article includes 13 references
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