The inhibitory effect of furosemide on the contractile response of equine trachealis to cholinergic nerve stimulation.
Abstract: The effects of furosemide on the responses of equine trachealis muscle with and without epithelium to electrical field stimulation (EFS) and exogenous acetylcholine (ACh) were investigated in organ baths. Tissues were pretreated with guanethidine and the parameters used for EFS were those previously demonstrated to activate postganglionic cholinergic neurons. In tissues with intact epithelium, furosemide (100 microM) shifted the frequency-response curve to the right. In the preparations without epithelium, furosemide did not affect the response to EFS. Neither in epithelium-on nor in epithelium-off tissues was the ACh dose-response curve affected by the administration of furosemide. We conclude that furosemide (100 microM) decreases the equine tracheal smooth muscle responses to EFS through inhibition of cholinergic neurotransmission, and that this effect is dependent on epithelium.
Publication Date: 1992-12-01 PubMed ID: 1477480DOI: 10.1016/0952-0600(92)90065-oGoogle Scholar: Lookup
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- Journal Article
Summary
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This study investigates the impact of a pharmaceutical substance, furosemide, on the contraction responsiveness of horse windpipe muscles when subjected to both electrical field stimulation and the presence of a neurotransmitter called acetylcholine.
Study Methods
- The scientists conducted their experiments on the tracheal smooth muscle, which is the muscle lining the windpipe, or trachea, taken from horses.
- They worked with two groups of these tissues – some that had an intact epithelium (outer layer of cells) and some where this was removed.
- The tissue samples were then pretreated with a substance called guanethidine, which is typically used to reduce the effect of noradrenaline, a neurotransmitter.
- These samples were later subjected to electrical field stimulation (EFS), a technique used to provoke cellular responses, in this case, contractions. This was done using parameters previously proven to activate cholinergic neurons, which respond to acetylcholine.
- Furthermore, they added acetylcholine directly to see any effects on the smooth muscle tissue behaviour.
- In all of these experiments, some tissue samples were treated with 100 microM of furosemide, while others were not, serving as control samples.
Key Findings
- In the samples with an intact epithelium, adding furosemide resulted in a shift of the response curve to the right. This indicates that the muscle responds more slowly or less strongly to stimulation when treated with furosemide.
- For those samples without an epithelium, no significant change was observed in the presence of furosemide.
- In neither scenarios (i.e., with or without epithelium), the researchers observed that the acetylcholine dose-response curve did not alter with the administration of furosemide.
Conclusion
- The findings suggest that the presence of furosemide decreases the response of equine tracheal smooth muscles to electrical field stimulation. This is likely due to the inhibition of cholinergic neurotransmission, as represented by the modified response curves.
- The role of the epithelium seems to be important in this mechanism since the effect of furosemide could not be observed in its absence.
Cite This Article
APA
Yu M, Wang Z, Robinson NE, Derksen FJ.
(1992).
The inhibitory effect of furosemide on the contractile response of equine trachealis to cholinergic nerve stimulation.
Pulm Pharmacol, 5(4), 233-238.
https://doi.org/10.1016/0952-0600(92)90065-o Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824-1314.
MeSH Terms
- Acetylcholine / pharmacology
- Animals
- Cholinergic Fibers / drug effects
- Drug Interactions
- Electric Stimulation
- Epithelium / drug effects
- Furosemide / pharmacology
- Horses
- Muscle Contraction / drug effects
- Muscle, Smooth / drug effects
- Trachea / drug effects
Citations
This article has been cited 1 times.- Verleden GM, Pype JL, Deneffe G, Demedts MG. Effect of loop diuretics on cholinergic neurotransmission in human airways in vitro. Thorax 1994 Jul;49(7):657-63.
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