The In Vitro Effect of Ozone Therapy Against Equine Pythium insidiosum.
Abstract: The goal of the present study was to characterize the antimicrobial action of different ozone (O) presentations against Pythium insidiosum isolated from horses. In experiment 1, P. insidiosum was treated with ozonated distilled water, ozonated sunflower oils with distinct peroxide indexes or O gas (72 μg O mL). In experiment 2, samples were exposed one or three times to oxygen (O) or O gas (72 μg O mL; 30 min/day). In experiment 3, P. insidiosum was treated with different concentrations of O gas (Ø, 32, 52, or 72 μg O mL) for three days (30 min/day). In experiment 4, samples were exposed to O or O gas (72 μg O mL) for 05, 15, or 30 minutes during three days. Posteriorly, all samples were cultured for two weeks, and the pathogen growth area was measured until D14. Samples with absence of growth on D14 were recultured to assess the germicidal or germistatic action of the treatment. In experiment 1, only ozonized sunflower oil with a high peroxide index had germicidal action against the pathogen. In experiment 2, samples exposed three times to O gas were inactivated. In experiment 3, the O therapy had germicidal action against P. insidiosum independently of the gas concentration (P > .1). In experiment 4, O treatments ≥15 minutes suppressed the pathogen development, whereas samples exposed to O gas for 5 minutes had progressive growth (P < .01). In conclusion, ozonated sunflower oil with a high peroxide index and multiple exposures to O gas mixtures were able to inactivate P. insidiosum isolated from horses.
Copyright © 2020 Elsevier Inc. All rights reserved.
Publication Date: 2020-12-30 PubMed ID: 33663716DOI: 10.1016/j.jevs.2020.103305Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research explored the impact of different ozone presentations on the growth of a pathogen called Pythium insidiosum, isolated from horses, and concluded that ozonated sunflower oil with a high peroxide index and multiple exposures to ozone gas were able to inhibit the growth of the pathogen.
Experiment design
- The researchers conducted a series of four experiments to explore varying aspects of the use of ozone as a therapeutic agent against Pythium insidiosum, a pathogen commonly found in horses.
- In each experiment, the samples of the pathogen were treated with different types of ozone presentations: ozonated distilled water, ozonated sunflower oil with different peroxide indexes, and ozone gas. The samples were then cultured for two weeks, and the growth of the pathogen was measured.
- After the initial two-week period, those samples that exhibited no growth were re-cultured to assess whether the ozone treatment had killed the pathogen (germicidal action) or just inhibited its growth without killing it (germistatic action).
Findings
- In the first experiment, only the ozonated sunflower oil with a high peroxide index demonstrated germicidal action against Pythium insidiosum.
- In the second experiment, it was discovered that exposing the pathogen samples three times to ozone gas inhibited their growth.
- In the third experiment, ozone therapy was found to kill the pathogen, regardless of the concentration of ozone gas used.
- The fourth experiment showed that ozone treatments lasting for fifteen minutes or more curbed pathogen growth. Conversely, samples exposed to ozone gas for just five minutes continued to grow.
Conclusion
- The researchers concluded that both the ozonated sunflower oil with a high peroxide index and multiple exposures to ozone gas were effective at inhibiting the growth of Pythium insidiosum, isolated from horses.
- This finding indicates that these methods of ozone therapy could potentially be used as treatment options for infections caused by this pathogen.
Cite This Article
APA
Ferreira JC, Pires RH, Costa GBD, Carrijo BN, Guiotto FG, Rodrigues VS.
(2020).
The In Vitro Effect of Ozone Therapy Against Equine Pythium insidiosum.
J Equine Vet Sci, 98, 103305.
https://doi.org/10.1016/j.jevs.2020.103305 Publication
Researcher Affiliations
- Post-Graduation Program in Animal Sciences, University of Franca, Brazil. Electronic address: jair.ferreira@unifran.edu.br.
- Post-Graduation Program in Health Promotion, University of Franca, Brazil.
- Post-Graduation Program in Animal Sciences, University of Franca, Brazil.
- Post-Graduation Program in Animal Sciences, University of Franca, Brazil.
- Post-Graduation Program in Animal Sciences, University of Franca, Brazil.
- Post-Graduation Program in Animal Sciences, University of Franca, Brazil.
MeSH Terms
- Animals
- Anti-Infective Agents
- Horse Diseases / drug therapy
- Horses
- Ozone / pharmacology
- Pythiosis
- Pythium
Citations
This article has been cited 3 times.- Yolanda H, Krajaejun T. History and Perspective of Immunotherapy for Pythiosis. Vaccines (Basel) 2021 Sep 26;9(10).
- Repciuc CC, Oros NV, Mureșan ȘMC, Sevastre B, Joaquim JGF, Oana LI. Efficacy of Ozone Bagging Therapy in Equine Chronic Distal Limb Wounds: Clinical Evaluation of Eight Cases. Vet Sci 2025 Dec 23;13(1).
- Braga CQ, Zambrano CG, Dos Santos Bermann C, Milech A, Ianiski LB, Soares MP, Pötter L, de Avila Botton S, Pereira DIB. In vitro and ex vivo anti-Pythium insidiosum potential of ozonated sunflower oil. Braz J Microbiol 2024 Mar;55(1):867-873.
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