The isolation and preliminary characterization of a rhabdovirus in Australia related to bovine ephemeral fever virus.
Abstract: CSIRO 368 virus was isolated from blood collected in the Northern Territory from a healthy cow and electron microscope studies showed that the isolate had rhabdovirus morphology. Fluorescent antibody studies and complement fixation tests related the virus to bovine ephemeral fever (BEF) virus. Neutralization tests in both suckling mice and Vero cells showed that the virus was not BEF virus. Antibodies to CSIRO 368 virus were found in cattle sera from northern and eastern Australia and Papua New Guinea. Antibodies were found in 16 out of 45 buffalo, some of which also had antibodies to BEF virus. In contrast, none of the 419 deer tested had antibodies to CSIRO 368 virus, although 142 of the same deer had antibodies to BEF virus. No antibodies to CSIRO 368 virus were detected in 16 goats, 54 horses, 10 pigs, 31 sheep, 25 kangaroos, or 14 human beings. Both CSIRO 368 and BEF viruses were found to be sensitive to ether and chloroform, but were not affected by the DNA inhibitor 5-bromo-2'-deoxyuridine, showing that they probably had the same type of nucleic acid--namely RNA. CSIRO 368 was also shown to grow to higher titres in BHK21 cells than in Vero cells. Temperature sensitivity studies at -20, 4 and 37 degrees C showed that the presence of foetal calf serum increased the survival time markedly at -20 degrees C, but only slightly at 4 and 37 degrees C. The virus survived the longest at -20 degrees C in the presence of foetal calf serum.
Publication Date: 1983-06-01 PubMed ID: 6612981DOI: 10.1016/0378-1135(83)90075-5Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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The research is about the isolation and initial characterization of the CSIRO 368 virus, a rhabdovirus associated with bovine ephemeral fever, discovered in a healthy cow in Australia’s Northern Territory.
Isolation and Characterization of CSIRO 368 Virus
- The research initially focuses on the isolation and preliminary description of the CSIRO 368 virus from a healthy cow’s blood sampled in Northern Territory, Australia. Electron microscopy confirmed the virus’s rhabdovirus-like morphology.
- Fluorescent antibody tests and complement fixation examinations associated the newly discovered virus with bovine ephemeral fever (BEF) virus, a disease causing high fever and lameness in cows. However, neutralization tests in suckling mice and Vero cells showed that this virus was not the BEF virus itself.
Antibody Presence in Different Species
- The researchers found antibodies to the CSIRO 368 virus in cattle sera from the northern and eastern parts of Australia and Papua New Guinea, indicating exposure to this virus.
- Antibodies were also present in 16 out of 45 buffaloes examined, some of whom also had antibodies to the BEF virus.
- Interestingly, none of the 419 deer tested had antibodies to the CSIRO 368 virus, despite 142 of them having antibodies to the BEF virus.
- The researchers also failed to detect CSIRO 368 virus antibodies in several other species, including goats, horses, pigs, sheep, and kangaroos, along with humans.
Virus Sensitivity and Survival
- The study revealed both the CSIRO 368 and the BEF viruses were susceptible to ether and chloroform, but unaffected by the DNA inhibitor 5-bromo-2′-deoxyuridine. This result suggests they both contain RNA as their genetic material.
- The CSIRO 368 virus was found to grow to higher levels in BHK21 cells compared to Vero cells.
- Temperature sensitivity tests indicated that the presence of fetal calf serum significantly enhanced the virus’s survival time at -20 degrees Celsius, with only a slight increase at temperatures of 4 and 37 degrees Celsius. The maximum survival of the virus was observed at -20 degrees Celsius in the presence of foetal calf serum.
Cite This Article
APA
Cybinski DH, Zakrzewski H.
(1983).
The isolation and preliminary characterization of a rhabdovirus in Australia related to bovine ephemeral fever virus.
Vet Microbiol, 8(3), 221-235.
https://doi.org/10.1016/0378-1135(83)90075-5 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Animals, Wild / immunology
- Antibodies, Viral / analysis
- Australia
- Blood / microbiology
- Cattle / immunology
- Cattle / microbiology
- Ephemeral Fever / microbiology
- Female
- Humans
- Rhabdoviridae / classification
- Rhabdoviridae / immunology
- Rhabdoviridae / isolation & purification
Citations
This article has been cited 9 times.- Behar A, Friedgut O, Rotenberg D, Zalesky O, Izhaki O, Yulzary A, Rot A, Wolkomirsky R, Zamir L, Hmd F, Brenner J. Insights on Transmission, Spread, and Possible Endemization of Selected Arboviruses in Israel-Interim Results from Five-Year Surveillance. Vet Sci 2022 Feb 2;9(2).
- Blasdell KR, Davis SS, Voysey R, Bulach DM, Middleton D, Williams S, Harmsen MB, Weir RP, Crameri S, Walsh SJ, Peck GR, Tesh RB, Boyle DB, Melville LF, Walker PJ. Hayes Yard virus: a novel ephemerovirus isolated from a bull with severe clinical signs of bovine ephemeral fever is most closely related to Puchong virus. Vet Res 2020 Apr 29;51(1):58.
- Walker PJ, Klement E. Epidemiology and control of bovine ephemeral fever. Vet Res 2015 Oct 28;46:124.
- Blasdell KR, Widen SG, Diviney SM, Firth C, Wood TG, Guzman H, Holmes EC, Tesh RB, Vasilakis N, Walker PJ. Koolpinyah and Yata viruses: two newly recognised ephemeroviruses from tropical regions of Australia and Africa. Vet Microbiol 2014 Dec 5;174(3-4):547-553.
- Geoghegan JL, Walker PJ, Duchemin JB, Jeanne I, Holmes EC. Seasonal drivers of the epidemiology of arthropod-borne viruses in Australia. PLoS Negl Trop Dis 2014 Nov;8(11):e3325.
- Trinidad L, Blasdell KR, Joubert DA, Davis SS, Melville L, Kirkland PD, Coulibaly F, Holmes EC, Walker PJ. Evolution of bovine ephemeral fever virus in the Australian episystem. J Virol 2014 Feb;88(3):1525-35.
- Chunling J, Junduan Y. Evidence of Kimberley virus infection of cattle in China. Trop Anim Health Prod 1989 Feb;21(1):85-6.
- Cybinski DH, Davis SS, Zakrzewski H. Antigenic variation of the bovine ephemeral fever virus glycoprotein. Arch Virol 1992;124(3-4):211-24.
- Tu Z, Wang T, Xu Y, Sun H, Peng P, Qin S, Tu C. Identification and genetic analysis of new ephemeroviruses in wild boars in China. Virol Sin 2025 Apr;40(2):186-191.
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