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Home / Equine Research Bank / Reprod Domest Anim / The NF-κB-signalling pathway in mare’s endometrium in...
Reproduction in domestic animals = Zuchthygiene2022; 57(6); 598-610; doi: 10.1111/rda.14099

The NF-κB-signalling pathway in mare’s endometrium infiltrated with the inflammatory cells.

Abstract: Endometritis is an important issue decreasing mares' fertility. In the case of endometritis, both inflammatory cells infiltration and proinflammatory molecules production are regulated by various cellular and gene regulatory mechanisms, including the nuclear factor-κB (NF-κB)-dependent pathway. NF-κB-signalling pathway has been recently studied in the equine endometrium in the context of endometrosis. Thus, this study aimed to determine gene transcription of NF-κB subunits (RelA; NF-κB1; NF-κB2), proinflammatory molecules (MCP-1; IL-6) and hyaluronan synthases (HAS 1; HAS 2; HAS 3) in endometritis and compare them with the intensity and type of inflammatory cell infiltration. Endometrial samples, collected post-mortem from cyclic mares in oestrus or dioestrus, were classified histologically and examined using quantitative PCR. Transcription NF-κB subunits genes did not differ with either inflammatory intensity or type of inflammatory cell infiltration. Transcription of MCP-1 and IL-6 genes increased with the severity of inflammation, with the involvement of HAS 3 and HAS 2 genes, as opposed to HAS 1 genes. These proinflammatory molecules and hyaluronan synthases in the equine inflamed endometrium do not seem to be regulated by the NF-κB pathway. Hence, separate signalling pathways for the development and progression of equine endometritis and endometrosis may be suggested.
© 2022 The Authors. Reproduction in Domestic Animals published by Wiley-VCH GmbH.
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Publication Date: 2022-02-25 PubMed ID: 35182075PubMed Central: PMC9305511DOI: 10.1111/rda.14099Google Scholar: Lookup
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Summary

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The research article revolves around an investigation concerning the NF-κB-signalling pathway’s influence on endometritis, a condition affecting mares’ fertility. This study examines the gene transcription of NF-κB subunits and proinflammatory molecules in the context of endometritis as well as its correlation with the severity of inflammation and the type of inflammatory cell infiltration.

Introduction to the Research Study

  • Endometritis is a condition that significantly impacts mares’ fertility. This condition is characterized by the invasion of inflammatory cells and the subsequent production of proinflammatory molecules. These processes are regulated by various cellular mechanisms and gene regulatory pathways, including the nuclear factor-κB (NF-κB) dependent pathway.
  • The focus of this study is to explore the NF-κB-signalling pathway’s role in the endometrium of horses, particularly in the context of endometritis.

Methodology and Data Collection

  • The researchers conducted an in-depth analysis of the gene transcription of NF-κB subunits (RelA; NF-κB1; NF-κB2), proinflammatory molecules (MCP-1; IL-6), and hyaluronan synthases (HAS 1; HAS 2; HAS 3) in cases of endometritis.
  • Endometrial samples were collected from cyclic mares in oestrus or dioestrus. Post mortem classification and inspection of these samples were carried out histologically and they were examined using quantitative PCR. This process allowed researchers to compare these factors with the severity and type of inflammatory cell infiltration in the equine endometrium.

Research Findings

  • The research revealed that the transcription of NF-κB subunits genes did not vary with levels of inflammation or the type of infiltrating inflammatory cells.
  • Gene transcription of proinflammatory molecules, MCP-1 and IL-6, increased in line with the severity of inflammation. Interestingly, this escalation was related to the operations of HAS 3 and HAS 2 genes, but not HAS 1 genes.
  • The study discovered that in equine inflamed endometrium, proinflammatory molecules and hyaluronan synthases do not seem to be regulated by the NF-κB pathway.

Conclusions

  • Based on the findings, the study infers that different signalling pathways may be implicated in the development and progression of equine endometritis and endometrosis. This suggests that the NF-κB pathway may not be a significant player in controlling inflammation levels in endometritis. It paves the way for more research to understand different signalling pathways in equine endometrial inflammation.

Cite This Article

APA
Jasiński T, Zdrojkowski Ł, Kautz E, Juszczuk-Kubiak E, Ferreira-Dias G, Domino M. (2022). The NF-κB-signalling pathway in mare’s endometrium infiltrated with the inflammatory cells. Reprod Domest Anim, 57(6), 598-610. https://doi.org/10.1111/rda.14099

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 57
Issue: 6
Pages: 598-610

Researcher Affiliations

Jasiński, Tomasz
  • Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences (WULS - SGGW), Warsaw, Poland.
Zdrojkowski, Łukasz
  • Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences (WULS - SGGW), Warsaw, Poland.
Kautz, Ewa
  • Department of Molecular Biology, Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Jastrzębiec, Poland.
Juszczuk-Kubiak, Edyta
  • Laboratory of Biotechnology and Molecular Engineering, Department of Microbiology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology-State Research Institute, Warsaw, Poland.
Ferreira-Dias, Graça
  • Departmento de Morfologia e Função, CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Lisbon, Portugal.
Domino, Małgorzata
  • Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences (WULS - SGGW), Warsaw, Poland.

MeSH Terms

  • Animals
  • Endometritis / pathology
  • Endometritis / veterinary
  • Endometrium / metabolism
  • Female
  • Horse Diseases / pathology
  • Horses
  • Hyaluronan Synthases / metabolism
  • Interleukin-6 / genetics
  • Interleukin-6 / metabolism
  • NF-kappa B / metabolism

Grant Funding

  • National Science Centre

Conflict of Interest Statement

None of the authors have any conflict of interest to declare.

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Citations

This article has been cited 4 times.
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  2. Sadowska A, Molcan T, Słyszewska M, Skarzynski DJ, Ferreira-Dias G, Szóstek-Mioduchowska A. Transcriptomic Insights into Tumor Necrosis Factor α's Role in the Fibrosis-Related Processes of Equine Endometrial Fibroblasts. Int J Mol Sci 2025 Nov 24;26(23).
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