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Home / Equine Research Bank / Osteoarthritis Cartilage / The OARSI histopathology initiative – recommendations ...
Osteoarthritis and cartilage2010; 18 Suppl 3; S93-S105; doi: 10.1016/j.joca.2010.05.031

The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the horse.

Abstract: Equine models of osteoarthritis (OA) have been used to investigate pathogenic pathways of OA and evaluate therapeutic candidates for naturally occurring equine OA which is a significant clinical disease in the horse. This review focuses on the macroscopic and microscopic criteria for assessing naturally occurring OA in the equine metacarpophalangeal joint as well as the osteochondral fragment-exercise model of OA in the equine middle carpal joint. Methods: A review was conducted of all published OA studies using horses and the most common macroscopic and microscopic scoring systems were summarized. Recommendations regarding methods of OA assessment in the horse have been made based on published studies. Results: A modified Mankin scoring system is recommended for semi-quantitative histological assessment of OA in horses due to its already widespread use and similarity to other scoring systems. Recommendations are also provided for histological scoring of synovitis and macroscopic lesions of OA as well as changes in the calcified cartilage and subchondral bone of naturally occurring OA. Conclusions: The proposed system for assessment of equine articular tissues provides a useful method to quantify OA change. It is believed that addition of quantitative tracing onto plastic and macroscopic measurement as recently described would be an improvement for overall assessment of articular cartilage change.
Copyright © 2010 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
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Publication Date: 2010-10-01 PubMed ID: 20864027DOI: 10.1016/j.joca.2010.05.031Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the use of a modified version of a common scoring system called the Mankin scoring system to assess the severity of osteoarthritis (OA) in horses. The scientists also make suggestions for grading synovitis and other OA-related changes in horses’ joints.

Understanding the Research

  • The research is based on reviewing previous studies of osteoarthritis (OA) in horses. It specifically focuses on OA in the equine metacarpophalangeal joint and the equine middle carpal joint.
  • The goal of the review was to summarize frequently used scoring systems for evaluating the severity of OA in horses and make recommendations for best practices based on these studies.
  • The scoring system recommended by the researchers for histological assessment (i.e., examining tissue samples under a microscope) of horse OA is a modified version of the Mankin scoring system, which is commonly used to evaluate OA in humans.

The Modified Mankin Scoring System

  • The Mankin scoring system measures changes in the structure of the joint, such as the thickness of the cartilage and the bone underneath it, the arrangement of cells within the cartilage, and the presence of cell clusters.
  • The researchers modified this scoring system so that it would be more suited to evaluating equine OA.

Additional Recommendations

  • In addition to recommending the use of the modified Mankin scoring system, the researchers also make suggestions for how to assess other changes caused by OA. These include changes in the synovium, a layer of tissue that surrounds joints, and the appearance of macroscopic (i.e., visible to the naked eye) lesions.
  • The researchers believe that adding quantitative tracing (measuring and recording changes over time) onto plastic, followed by making macroscopic measurements, could improve the overall evaluation of changes in joint tissues.

Conclusion

  • The researchers conclude that their proposed system for evaluating OA in horses offers a necessary method for quantifying changes in joint tissues due to OA. This system could improve understanding of OA pathology and help evaluate the efficacy of OA treatment strategies in horses.

Cite This Article

APA
McIlwraith CW, Frisbie DD, Kawcak CE, Fuller CJ, Hurtig M, Cruz A. (2010). The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the horse. Osteoarthritis Cartilage, 18 Suppl 3, S93-S105. https://doi.org/10.1016/j.joca.2010.05.031

Publication

Osteoarthritis and cartilage
ISSN: 1522-9653
NlmUniqueID: 9305697
Country: England
Language: English
Volume: 18 Suppl 3
Pages: S93-S105

Researcher Affiliations

McIlwraith, C W
  • Orthopaedic Research Center, Colorado State University, 300 West Drake, Fort Collins, CO 80523, USA. wayne.mcilwraith@colostate.edu
Frisbie, D D
    Kawcak, C E
      Fuller, C J
        Hurtig, M
          Cruz, A

            MeSH Terms

            • Animals
            • Arthritis, Experimental / pathology
            • Cartilage, Articular / pathology
            • Disease Models, Animal
            • Horses
            • Joints / pathology
            • Osteoarthritis / pathology
            • Severity of Illness Index
            • Synovial Membrane / pathology

            Citations

            This article has been cited 75 times.
            1. Thampi P, Seabaugh KA, Pezzanite LM, Chu CR, Phillips JN, Grieger JC, McIlwraith CW, Samulski RJ, Goodrich LR. A pilot study to determine the optimal dose of scAAVIL-1ra in a large animal model of post-traumatic osteoarthritis.. Gene Ther 2023 Sep 11;.
              doi: 10.1038/s41434-023-00420-2pubmed: 37696981google scholar: lookup
            2. Dou H, Wang S, Hu J, Song J, Zhang C, Wang J, Xiao L. Osteoarthritis models: From animals to tissue engineering.. J Tissue Eng 2023 Jan-Dec;14:20417314231172584.
              doi: 10.1177/20417314231172584pubmed: 37223125google scholar: lookup
            3. Oka Y, Murata K, Ozone K, Minegishi Y, Kano T, Shimada N, Kanemura N. Mild treadmill exercise inhibits cartilage degeneration via macrophages in an osteoarthritis mouse model.. Osteoarthr Cartil Open 2023 Jun;5(2):100359.
              doi: 10.1016/j.ocarto.2023.100359pubmed: 37180812google scholar: lookup
            4. Chu K, Zhu Y, Lu G, Huang S, Yang C, Zheng J, Chen J, Ban J, Jia H, Lu Z. Formation of Hydrophilic Nanofibers from Nanostructural Design in the Co-Encapsulation of Celecoxib through Electrospinning.. Pharmaceutics 2023 Feb 22;15(3).
              doi: 10.3390/pharmaceutics15030730pubmed: 36986590google scholar: lookup
            5. Mayet A, Zablotski Y, Roth SP, Brehm W, Troillet A. Systematic review and meta-analysis of positive long-term effects after intra-articular administration of orthobiologic therapeutics in horses with naturally occurring osteoarthritis.. Front Vet Sci 2023;10:1125695.
              doi: 10.3389/fvets.2023.1125695pubmed: 36908512google scholar: lookup
            6. Noordwijk KJ, Chen L, Ruspi BD, Schurer S, Papa B, Fasanello DC, McDonough SP, Palmer SE, Porter IR, Basran PS, Donnelly E, Reesink HL. Metacarpophalangeal Joint Pathology and Bone Mineral Density Increase with Exercise but Not with Incidence of Proximal Sesamoid Bone Fracture in Thoroughbred Racehorses.. Animals (Basel) 2023 Feb 24;13(5).
              doi: 10.3390/ani13050827pubmed: 36899684google scholar: lookup
            7. Keller LE, Tait Wojno ED, Begum L, Fortier LA. T Helper 17-Like Regulatory T Cells in Equine Synovial Fluid Are Associated With Disease Severity of Naturally Occurring Posttraumatic Osteoarthritis.. Am J Sports Med 2023 Mar;51(4):1047-1058.
              doi: 10.1177/03635465231153588pubmed: 36794851google scholar: lookup
            8. Baker ME, Lee S, Clinton M, Hackl M, Castanheira C, Peffers MJ, Taylor SE. Investigation of MicroRNA Biomarkers in Equine Distal Interphalangeal Joint Osteoarthritis.. Int J Mol Sci 2022 Dec 8;23(24).
              doi: 10.3390/ijms232415526pubmed: 36555166google scholar: lookup
            9. Stewart HL, Easley JT, Selberg KT, Puttlitz CM, Nakamura LK, Johnson JW, Kawcak CE. Experimental models of bone marrow lesions in ovine femoral condyles.. Vet Surg 2023 Feb;52(2):284-298.
              doi: 10.1111/vsu.13919pubmed: 36523261google scholar: lookup
            10. Mobasheri A, Im GI, Katz JN, Loughlin J, Kraus VB, Sandell LJ, Berenbaum F, Abramson S, Lotz M, Hochberg M, Pelletier JP, Madry H, Block JA, Lohmander LS, Altman RD. Osteoarthritis Research Society International (OARSI): Past, present and future.. Osteoarthr Cartil Open 2021 Jun;3(2):100146.
              doi: 10.1016/j.ocarto.2021.100146pubmed: 36474983google scholar: lookup
            11. Pezzanite LM, Chow L, Phillips J, Griffenhagen GM, Moore AR, Schaer TP, Engiles JB, Werpy N, Gilbertie J, Schnabel LV, Antczak D, Miller D, Dow S, Goodrich LR. TLR-activated mesenchymal stromal cell therapy and antibiotics to treat multi-drug resistant Staphylococcal septic arthritis in an equine model.. Ann Transl Med 2022 Nov;10(21):1157.
              doi: 10.21037/atm-22-1746pubmed: 36467344google scholar: lookup
            12. Mustonen AM, Lehmonen N, Oikari S, Capra J, Raekallio M, Mykkänen A, Paakkonen T, Rilla K, Niemelä T, Nieminen P. Counts of hyaluronic acid-containing extracellular vesicles decrease in naturally occurring equine osteoarthritis.. Sci Rep 2022 Oct 20;12(1):17550.
              doi: 10.1038/s41598-022-21398-8pubmed: 36266410google scholar: lookup
            13. Clarke EJ, Lima C, Anderson JR, Castanheira C, Beckett A, James V, Hyett J, Goodacre R, Peffers MJ. Optical photothermal infrared spectroscopy can differentiate equine osteoarthritic plasma extracellular vesicles from healthy controls.. Anal Methods 2022 Sep 29;14(37):3661-3670.
              doi: 10.1039/d2ay00779gpubmed: 36066093google scholar: lookup
            14. Anderson JR, Jacobsen S, Walters M, Bundgaard L, Diendorfer A, Hackl M, Clarke EJ, James V, Peffers MJ. Small non-coding RNA landscape of extracellular vesicles from a post-traumatic model of equine osteoarthritis.. Front Vet Sci 2022;9:901269.
              doi: 10.3389/fvets.2022.901269pubmed: 36003409google scholar: lookup
            15. Andersen C, Jacobsen S, Walters M, Lindegaard C. A detailed macroscopic scoring system for experimental post-traumatic Osteoarthritis in the equine middle carpal joint.. BMC Res Notes 2022 Jun 27;15(1):226.
              doi: 10.1186/s13104-022-06116-xpubmed: 35761416google scholar: lookup
            16. Seabaugh KA, Barrett MF, Rao S, McIlwraith CW, Frisbie DD. Examining the Effects of the Oral Supplement Biota orientalis in the Osteochondral Fragment-Exercise Model of Osteoarthritis in the Horse.. Front Vet Sci 2022;9:858391.
              doi: 10.3389/fvets.2022.858391pubmed: 35720848google scholar: lookup
            17. Gilbertie JM, Schaer TP, Engiles JB, Seiler GS, Deddens BL, Schubert AG, Jacob ME, Stefanovski D, Ruthel G, Hickok NJ, Stowe DM, Frink A, Schnabel LV. A Platelet-Rich Plasma-Derived Biologic Clears Staphylococcus aureus Biofilms While Mitigating Cartilage Degeneration and Joint Inflammation in a Clinically Relevant Large Animal Infectious Arthritis Model.. Front Cell Infect Microbiol 2022;12:895022.
              doi: 10.3389/fcimb.2022.895022pubmed: 35711655google scholar: lookup
            18. Yamada ALM, do Prado Vendruscolo C, Marsiglia MF, Sotelo EDP, Agreste FR, Seidel SRT, Fülber J, Baccarin RYA, da Silva LCLC. Effects of oral treatment with chondroitin sulfate and glucosamine in an experimental model of metacarpophalangeal osteoarthritis in horses.. BMC Vet Res 2022 Jun 9;18(1):215.
              doi: 10.1186/s12917-022-03323-3pubmed: 35681208google scholar: lookup
            19. Kendall A, Ekman S, Skiöldebrand E. Nerve growth factor receptors in equine synovial membranes vary with osteoarthritic disease severity.. J Orthop Res 2023 Feb;41(2):316-324.
              doi: 10.1002/jor.25382pubmed: 35578994google scholar: lookup
            20. Löfgren M, Ekman S, Ekholm J, Engström M, Fjordbakk CT, Svala E, Holm Forsström K, Lindahl A, Skiöldebrand E. Conditioned serum in vitro treatment of chondrocyte pellets and osteoarthritic explants.. Equine Vet J 2023 Mar;55(2):325-335.
              doi: 10.1111/evj.13582pubmed: 35514185google scholar: lookup
            21. Pagliara E, Pasinato A, Valazza A, Riccio B, Cantatore F, Terzini M, Putame G, Parrilli A, Sartori M, Fini M, Zanetti EM, Bertuglia A. Multibody Computer Model of the Entire Equine Forelimb Simulates Forces Causing Catastrophic Fractures of the Carpus during a Traditional Race.. Animals (Basel) 2022 Mar 16;12(6).
              doi: 10.3390/ani12060737pubmed: 35327134google scholar: lookup
            22. McCoy AM, Secor EJ, Roady PJ, Gray SM, Klein J, Gutierrez-Nibeyro SD. Plantar osteochondral fragments in young Standardbreds are associated with minimal joint inflammation at the time of surgical removal.. Equine Vet J 2023 Jan;55(1):33-41.
              doi: 10.1111/evj.13575pubmed: 35298851google scholar: lookup
            23. Cokelaere SM, Groen WMGAC, Plomp SGM, de Grauw JC, van Midwoud PM, Weinans HH, van de Lest CHA, Tryfonidou MA, van Weeren PR, Korthagen NM. Sustained Intra-Articular Release and Biocompatibility of Tacrolimus (FK506) Loaded Monospheres Composed of [PDLA-PEG(1000)]-b-[PLLA] Multi-Block Copolymers in Healthy Horse Joints.. Pharmaceutics 2021 Sep 10;13(9).
              doi: 10.3390/pharmaceutics13091438pubmed: 34575514google scholar: lookup
            24. Kroupa KR, Wu MI, Zhang J, Jensen M, Wong W, Engiles JB, Schaer TP, Grinstaff MW, Snyder BD, Bergholt MS, Albro MB. Raman needle arthroscopy for in vivo molecular assessment of cartilage.. J Orthop Res 2022 Jun;40(6):1338-1348.
              doi: 10.1002/jor.25155pubmed: 34370873google scholar: lookup
            25. Menarim BC, MacLeod JN, Dahlgren LA. Bone marrow mononuclear cells for joint therapy: The role of macrophages in inflammation resolution and tissue repair.. World J Stem Cells 2021 Jul 26;13(7):825-840.
              doi: 10.4252/wjsc.v13.i7.825pubmed: 34367479google scholar: lookup
            26. Di Francesco M, Bedingfield SK, Di Francesco V, Colazo JM, Yu F, Ceseracciu L, Bellotti E, Di Mascolo D, Ferreira M, Himmel LE, Duvall C, Decuzzi P. Shape-Defined microPlates for the Sustained Intra-articular Release of Dexamethasone in the Management of Overload-Induced Osteoarthritis.. ACS Appl Mater Interfaces 2021 Jul 14;13(27):31379-31392.
              doi: 10.1021/acsami.1c02082pubmed: 34197081google scholar: lookup
            27. Boyde A. The Bone Cartilage Interface and Osteoarthritis.. Calcif Tissue Int 2021 Sep;109(3):303-328.
              doi: 10.1007/s00223-021-00866-9pubmed: 34086084google scholar: lookup
            28. Engelhardt JP, Schütte A, Hetjens S, Reisig G, Schwarz ML. Resilience to height loss of articular cartilage of osteoarthritic stifle joints of old pigs, compared with healthy cartilage from young pigs in a tribological pin-on-plate exposure, revealing similar friction forces.. PLoS One 2021;16(4):e0250244.
              doi: 10.1371/journal.pone.0250244pubmed: 33891624google scholar: lookup
            29. Bertoni L, Jacquet-Guibon S, Branly T, Desancé M, Legendre F, Melin M, Rivory P, Hartmann DJ, Schmutz A, Denoix JM, Demoor M, Audigié F, Galéra P. Evaluation of Allogeneic Bone-Marrow-Derived and Umbilical Cord Blood-Derived Mesenchymal Stem Cells to Prevent the Development of Osteoarthritis in An Equine Model.. Int J Mol Sci 2021 Mar 2;22(5).
              doi: 10.3390/ijms22052499pubmed: 33801461google scholar: lookup
            30. Nelson BB, Stewart RC, Kawcak CE, Freedman JD, Patwa AN, Snyder BD, Goodrich LR, Grinstaff MW. Quantitative Evaluation of Equine Articular Cartilage Using Cationic Contrast-Enhanced Computed Tomography.. Cartilage 2021 Apr;12(2):211-221.
              doi: 10.1177/1947603518812562pubmed: 33722083google scholar: lookup
            31. Camargo Garbin L, Morris MJ. A Comparative Review of Autologous Conditioned Serum and Autologous Protein Solution for Treatment of Osteoarthritis in Horses.. Front Vet Sci 2021;8:602978.
              doi: 10.3389/fvets.2021.602978pubmed: 33681323google scholar: lookup
            32. Castanheira C, Balaskas P, Falls C, Ashraf-Kharaz Y, Clegg P, Burke K, Fang Y, Dyer P, Welting TJM, Peffers MJ. Equine synovial fluid small non-coding RNA signatures in early osteoarthritis.. BMC Vet Res 2021 Jan 9;17(1):26.
              doi: 10.1186/s12917-020-02707-7pubmed: 33422071google scholar: lookup
            33. Te Moller NCR, Mohammadi A, Plomp S, Serra Bragança FM, Beukers M, Pouran B, Afara IO, Nippolainen E, Mäkelä JTA, Korhonen RK, Töyräs J, Brommer H, van Weeren PR. Structural, compositional, and functional effects of blunt and sharp cartilage damage on the joint: A 9-month equine groove model study.. J Orthop Res 2021 Nov;39(11):2363-2375.
              doi: 10.1002/jor.24971pubmed: 33368588google scholar: lookup
            34. Menarim BC, Gillis KH, Oliver A, Ngo Y, Werre SR, Barrett SH, Rodgerson DH, Dahlgren LA. Macrophage Activation in the Synovium of Healthy and Osteoarthritic Equine Joints.. Front Vet Sci 2020;7:568756.
              doi: 10.3389/fvets.2020.568756pubmed: 33324696google scholar: lookup
            35. Ribitsch I, Baptista PM, Lange-Consiglio A, Melotti L, Patruno M, Jenner F, Schnabl-Feichter E, Dutton LC, Connolly DJ, van Steenbeek FG, Dudhia J, Penning LC. Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do.. Front Bioeng Biotechnol 2020;8:972.
              doi: 10.3389/fbioe.2020.00972pubmed: 32903631google scholar: lookup
            36. Anderson JR, Phelan MM, Foddy L, Clegg PD, Peffers MJ. Ex Vivo Equine Cartilage Explant Osteoarthritis Model: A Metabolomics and Proteomics Study.. J Proteome Res 2020 Sep 4;19(9):3652-3667.
              doi: 10.1021/acs.jproteome.0c00143pubmed: 32701294google scholar: lookup
            37. Bertoni L, Jacquet-Guibon S, Branly T, Legendre F, Desancé M, Mespoulhes C, Melin M, Hartmann DJ, Schmutz A, Denoix JM, Galéra P, Demoor M, Audigié F. An experimentally induced osteoarthritis model in horses performed on both metacarpophalangeal and metatarsophalangeal joints: Technical, clinical, imaging, biochemical, macroscopic and microscopic characterization.. PLoS One 2020;15(6):e0235251.
              doi: 10.1371/journal.pone.0235251pubmed: 32584901google scholar: lookup
            38. Pezzanite L, Chow L, Piquini G, Griffenhagen G, Ramirez D, Dow S, Goodrich L. Use of in vitro assays to identify antibiotics that are cytotoxic to normal equine chondrocytes and synovial cells.. Equine Vet J 2021 May;53(3):579-589.
              doi: 10.1111/evj.13314pubmed: 32544273google scholar: lookup
            39. Lacitignola L, Imperante A, Staffieri F, De Siena R, De Luca P, Muci A, Crovace A. Assessment of Intra- and Inter-observer Measurement Variability in a Radiographic Metacarpophalangeal Joint Osteophytosis Scoring System for the Horse.. Vet Sci 2020 Apr 6;7(2).
              doi: 10.3390/vetsci7020039pubmed: 32268589google scholar: lookup
            40. Anderson JR, Phelan MM, Rubio-Martinez LM, Fitzgerald MM, Jones SW, Clegg PD, Peffers MJ. Optimization of Synovial Fluid Collection and Processing for NMR Metabolomics and LC-MS/MS Proteomics.. J Proteome Res 2020 Jul 2;19(7):2585-2597.
              doi: 10.1021/acs.jproteome.0c00035pubmed: 32227958google scholar: lookup
            41. Braucke AFGV, Frederiksen NL, Berg LC, Aarsvold S, Müller FC, Boesen MP, Lindegaard C. Identification and Quantification of Transient Receptor Potential Vanilloid 1 (TRPV1) in Equine Articular Tissue.. Animals (Basel) 2020 Mar 18;10(3).
              doi: 10.3390/ani10030506pubmed: 32197454google scholar: lookup
            42. Samvelyan HJ, Hughes D, Stevens C, Staines KA. Models of Osteoarthritis: Relevance and New Insights.. Calcif Tissue Int 2021 Sep;109(3):243-256.
              doi: 10.1007/s00223-020-00670-xpubmed: 32062692google scholar: lookup
            43. Moss KL, Jiang Z, Dodson ME, Linardi RL, Haughan J, Gale AL, Grzybowski C, Engiles JE, Stefanovski D, Robinson MA, Ortved KF. Sustained Interleukin-10 Transgene Expression Following Intra-Articular AAV5-IL-10 Administration to Horses.. Hum Gene Ther 2020 Jan;31(1-2):110-118.
              doi: 10.1089/hum.2019.195pubmed: 31773987google scholar: lookup
            44. Oka Y, Murata K, Kano T, Ozone K, Arakawa K, Kokubun T, Kanemura N. Impact of Controlling Abnormal Joint Movement on the Effectiveness of Subsequent Exercise Intervention in Mouse Models of Early Knee Osteoarthritis.. Cartilage 2021 Dec;13(2_suppl):1334S-1344S.
              doi: 10.1177/1947603519885007pubmed: 31718284google scholar: lookup
            45. Anderson JR, Smagul A, Simpson D, Clegg PD, Rubio-Martinez LM, Peffers MJ. The synovial fluid proteome differentiates between septic and nonseptic articular pathologies.. J Proteomics 2019 Jun 30;202:103370.
              doi: 10.1016/j.jprot.2019.04.020pubmed: 31028944google scholar: lookup
            46. Broeckx SY, Martens AM, Bertone AL, Van Brantegem L, Duchateau L, Van Hecke L, Dumoulin M, Oosterlinck M, Chiers K, Hussein H, Pille F, Spaas JH. The use of equine chondrogenic-induced mesenchymal stem cells as a treatment for osteoarthritis: A randomised, double-blinded, placebo-controlled proof-of-concept study.. Equine Vet J 2019 Nov;51(6):787-794.
              doi: 10.1111/evj.13089pubmed: 30815897google scholar: lookup
            47. Harrison-Brown M, Scholes C, Hafsi K, Marenah M, Li J, Hassan F, Maffulli N, Murrell WD. Efficacy and safety of culture-expanded, mesenchymal stem/stromal cells for the treatment of knee osteoarthritis: a systematic review protocol.. J Orthop Surg Res 2019 Jan 25;14(1):34.
              doi: 10.1186/s13018-019-1070-8pubmed: 30683159google scholar: lookup
            48. Reesink HL, Nixon AJ, Su J, Liu S, Sutton RM, Mann S, Watts AE, Peterson RP. Galectins-1 and-3 Increase in Equine Post-traumatic Osteoarthritis.. Front Vet Sci 2018;5:288.
              doi: 10.3389/fvets.2018.00288pubmed: 30525048google scholar: lookup
            49. Stewart HL, Kawcak CE. The Importance of Subchondral Bone in the Pathophysiology of Osteoarthritis.. Front Vet Sci 2018;5:178.
              doi: 10.3389/fvets.2018.00178pubmed: 30211173google scholar: lookup
            50. Barrachina L, Remacha AR, Romero A, Vitoria A, Albareda J, Prades M, Roca M, Zaragoza P, Vázquez FJ, Rodellar C. Assessment of effectiveness and safety of repeat administration of proinflammatory primed allogeneic mesenchymal stem cells in an equine model of chemically induced osteoarthritis.. BMC Vet Res 2018 Aug 17;14(1):241.
              doi: 10.1186/s12917-018-1556-3pubmed: 30119668google scholar: lookup
            51. Morgan RE, Clegg PD, Hunt JA, Innes JF, Tew SR. Interaction with macrophages attenuates equine fibroblast-like synoviocyte ADAMTS5 (aggrecanase-2) gene expression following inflammatory stimulation.. J Orthop Res 2018 Mar 9;36(8):2178-85.
              doi: 10.1002/jor.23891pubmed: 29521434google scholar: lookup
            52. Rakic R, Bourdon B, Hervieu M, Branly T, Legendre F, Saulnier N, Audigié F, Maddens S, Demoor M, Galera P. RNA Interference and BMP-2 Stimulation Allows Equine Chondrocytes Redifferentiation in 3D-Hypoxia Cell Culture Model: Application for Matrix-Induced Autologous Chondrocyte Implantation.. Int J Mol Sci 2017 Aug 24;18(9).
              doi: 10.3390/ijms18091842pubmed: 28837082google scholar: lookup
            53. Nganvongpanit K, Soponteerakul R, Kaewkumpai P, Punyapornwithaya V, Buddhachat K, Nomsiri R, Kaewmong P, Kittiwatanawong K, Chawangwongsanukun R, Angkawanish T, Thitaram C, Mahakkanukrauh P. Osteoarthritis in two marine mammals and 22 land mammals: learning from skeletal remains.. J Anat 2017 Jul;231(1):140-155.
              doi: 10.1111/joa.12620pubmed: 28542897google scholar: lookup
            54. Kuemmerle JM, Theiss F, Okoniewski MJ, Weber FA, Hemmi S, Mirsaidi A, Richards PJ, Cinelli P. Identification of Novel Equine (Equus caballus) Tendon Markers Using RNA Sequencing.. Genes (Basel) 2016 Nov 10;7(11).
              doi: 10.3390/genes7110097pubmed: 27834918google scholar: lookup
            55. Reisig G, Kreinest M, Richter W, Wagner-Ecker M, Dinter D, Attenberger U, Schneider-Wald B, Fickert S, Schwarz ML. Osteoarthritis in the Knee Joints of Göttingen Minipigs after Resection of the Anterior Cruciate Ligament? Missing Correlation of MRI, Gene and Protein Expression with Histological Scoring.. PLoS One 2016;11(11):e0165897.
              doi: 10.1371/journal.pone.0165897pubmed: 27820852google scholar: lookup
            56. Kloefkorn HE, Allen KD. Quantitative histological grading methods to assess subchondral bone and synovium changes subsequent to medial meniscus transection in the rat.. Connect Tissue Res 2017 May-Jul;58(3-4):373-385.
              doi: 10.1080/03008207.2016.1251425pubmed: 27797605google scholar: lookup
            57. Delco ML, Kennedy JG, Bonassar LJ, Fortier LA. Post-traumatic osteoarthritis of the ankle: A distinct clinical entity requiring new research approaches.. J Orthop Res 2017 Mar;35(3):440-453.
              doi: 10.1002/jor.23462pubmed: 27764893google scholar: lookup
            58. Liu Z, Hu X, Man Z, Zhang J, Jiang Y, Ao Y. A novel rabbit model of early osteoarthritis exhibits gradual cartilage degeneration after medial collateral ligament transection outside the joint capsule.. Sci Rep 2016 Oct 19;6:34423.
              doi: 10.1038/srep34423pubmed: 27756901google scholar: lookup
            59. Cucchiarini M, de Girolamo L, Filardo G, Oliveira JM, Orth P, Pape D, Reboul P. Basic science of osteoarthritis.. J Exp Orthop 2016 Dec;3(1):22.
              doi: 10.1186/s40634-016-0060-6pubmed: 27624438google scholar: lookup
            60. Carmona JU, Ríos DL, López C, Álvarez ME, Pérez JE, Bohórquez ME. In vitro effects of platelet-rich gel supernatants on histology and chondrocyte apoptosis scores, hyaluronan release and gene expression of equine cartilage explants challenged with lipopolysaccharide.. BMC Vet Res 2016 Jul 1;12(1):135.
              doi: 10.1186/s12917-016-0759-8pubmed: 27369779google scholar: lookup
            61. Smith AD, Morton AJ, Winter MD, Colahan PT, Ghivizzani S, Brown MP, Hernandez JA, Nickerson DM. MAGNETIC RESONANCE IMAGING SCORING OF AN EXPERIMENTAL MODEL OF POST-TRAUMATIC OSTEOARTHRITIS IN THE EQUINE CARPUS.. Vet Radiol Ultrasound 2016 Sep;57(5):502-14.
              doi: 10.1111/vru.12369pubmed: 27198611google scholar: lookup
            62. Madry H, Ochi M, Cucchiarini M, Pape D, Seil R. Large animal models in experimental knee sports surgery: focus on clinical translation.. J Exp Orthop 2015 Dec;2(1):9.
              doi: 10.1186/s40634-015-0025-1pubmed: 26914877google scholar: lookup
            63. Kuyinu EL, Narayanan G, Nair LS, Laurencin CT. Animal models of osteoarthritis: classification, update, and measurement of outcomes.. J Orthop Surg Res 2016 Feb 2;11:19.
              doi: 10.1186/s13018-016-0346-5pubmed: 26837951google scholar: lookup
            64. Kwok J, Onuma H, Olmer M, Lotz MK, Grogan SP, D'Lima DD. Histopathological analyses of murine menisci: implications for joint aging and osteoarthritis.. Osteoarthritis Cartilage 2016 Apr;24(4):709-18.
              doi: 10.1016/j.joca.2015.11.006pubmed: 26585241google scholar: lookup
            65. Yu H, Ye WB, Zhong ZM, Ding RT, Chen JT. Effect of advanced oxidation protein products on articular cartilage and synovium in a rabbit osteoarthritis model.. Orthop Surg 2015 May;7(2):161-7.
              doi: 10.1111/os.12179pubmed: 26033998google scholar: lookup
            66. Maninchedda U, Lepage OM, Gangl M, Hilairet S, Remandet B, Meot F, Penarier G, Segard E, Cortez P, Jorgensen C, Steinberg R. Development of an equine groove model to induce metacarpophalangeal osteoarthritis: a pilot study on 6 horses.. PLoS One 2015;10(2):e0115089.
              doi: 10.1371/journal.pone.0115089pubmed: 25680102google scholar: lookup
            67. Thysen S, Luyten FP, Lories RJ. Targets, models and challenges in osteoarthritis research.. Dis Model Mech 2015 Jan;8(1):17-30.
              doi: 10.1242/dmm.016881pubmed: 25561745google scholar: lookup
            68. Duesterdieck-Zellmer KF, Moneta L, Ott JF, Larson MK, Gorman EM, Hunter B, Löhr CV, Payton ME, Morré JT, Maier CS. Effects of low and high dose intraarticular tiludronate on synovial fluid and clinical variables in healthy horses-a preliminary investigation.. PeerJ 2014;2:e534.
              doi: 10.7717/peerj.534pubmed: 25237596google scholar: lookup
            69. Boyde A, Davis GR, Mills D, Zikmund T, Cox TM, Adams VL, Niker A, Wilson PJ, Dillon JP, Ranganath LR, Jeffery N, Jarvis JC, Gallagher JA. On fragmenting, densely mineralised acellular protrusions into articular cartilage and their possible role in osteoarthritis.. J Anat 2014 Oct;225(4):436-46.
              doi: 10.1111/joa.12226pubmed: 25132002google scholar: lookup
            70. Peffers MJ, Cillero-Pastor B, Eijkel GB, Clegg PD, Heeren RM. Matrix assisted laser desorption ionization mass spectrometry imaging identifies markers of ageing and osteoarthritic cartilage.. Arthritis Res Ther 2014 May 9;16(3):R110.
              doi: 10.1186/ar4560pubmed: 24886698google scholar: lookup
            71. Hargrave-Thomas EJ, Thambyah A, McGlashan SR, Broom ND. The bovine patella as a model of early osteoarthritis.. J Anat 2013 Dec;223(6):651-64.
              doi: 10.1111/joa.12115pubmed: 24111904google scholar: lookup
            72. McIlwraith CW, Frisbie DD, Kawcak CE. The horse as a model of naturally occurring osteoarthritis.. Bone Joint Res 2012 Nov;1(11):297-309.
              doi: 10.1302/2046-3758.111.2000132pubmed: 23610661google scholar: lookup
            73. Boyce MK, Trumble TN, Carlson CS, Groschen DM, Merritt KA, Brown MP. Non-terminal animal model of post-traumatic osteoarthritis induced by acute joint injury.. Osteoarthritis Cartilage 2013 May;21(5):746-55.
              doi: 10.1016/j.joca.2013.02.653pubmed: 23467035google scholar: lookup
            74. Lee CM, Kisiday JD, McIlwraith CW, Grodzinsky AJ, Frisbie DD. Synoviocytes protect cartilage from the effects of injury in vitro.. BMC Musculoskelet Disord 2013 Feb 1;14:54.
              doi: 10.1186/1471-2474-14-54pubmed: 23374282google scholar: lookup
            75. Gregory MH, Capito N, Kuroki K, Stoker AM, Cook JL, Sherman SL. A review of translational animal models for knee osteoarthritis.. Arthritis 2012;2012:764621.
              doi: 10.1155/2012/764621pubmed: 23326663google scholar: lookup
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