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Reproduction in domestic animals = Zuchthygiene2019; 54(12); 1543-1551; doi: 10.1111/rda.13561

The preliminary studies on protein profile in retained and not retained foetal membranes in heavy draft mares.

Abstract: Protein profile of the placenta expresses its function and maintenance. Any alterations can be reflected in qualitative and quantitative changes in this profile. The aim of the present study was the evaluation of protein profile in the placenta of mares suffering from the retention of foetal membranes (FMR) by two separation methods and the comparison with physiologically released tissues. Placentas from 14 healthy, heavy draft mares were collected immediately after the expulsion of newborn. Tissues after homogenization and staining with fluorescent dyes were subjected to electrophoretic as well as chromatographic separation. Electrophoretic gels were statistically analysed for the presence and abundance of examined proteins, while some proteins were identified in chromatographic fractions. Out of 248 spots detected in endometrium, 38 differed significantly between FMR and control animals, while in allantochorion, respective values reached 241 and 27 spots (p < .05). Among identified proteins that expressed higher abundance in endometrium of FMR mares than control animals were prostaglandin reductase, dehydrogenase/reductase SDR family, and placental growth factor. These proteins are involved in regulation of parturition. Additionally, the following proteins responsible for physiological activity of a cell-guanine methyl transferase, aspartyl/asparaginyl beta-hydroxylase and GTP-binding protein, were identified. These proteins expressed higher abundance in allantochorion of FMR mares than in controls. This preliminary study confirmed the disturbances in protein pattern between foetal membranes in FMR and healthy mares. Further qualitative and quantitative experiments are necessary to deepen our knowledge on the mechanisms of the retention of foetal membranes in mares.
© 2019 Blackwell Verlag GmbH.
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Publication Date: 2019-09-14 PubMed ID: 31520443DOI: 10.1111/rda.13561Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the protein profile of the placenta in mares with retained foetal membranes (FMR). The study found disturbances in the protein pattern indicating that the retention of foetal membranes can be accompanied by changes in the protein profile.

Research Objectives

  • The study aimed to evaluate the protein profile in the placenta of mares suffering from the retention of foetal membranes (FMR).
  • It sought to compare the protein profiles between mares with FMR and those with physically released tissues.

Methodology

  • Placentas from 14 healthy, heavy draft mares were collected immediately after the expulsion of the newborn.
  • After staining with fluorescent dyes, the tissues were subjected to electrophoretic and chromatographic separation.
  • Electrophoretic gels were statistically analysed to identify and assess the abundance of examined proteins.

Findings

  • The study detected differences in protein spots between mares with FMR and control animals.
  • The identified proteins in the endometrium of FMR mares, were prostaglandin reductase, dehydrogenase/reductase SDR family, and placental growth factor. These are proteins involved in regulation of parturition.
  • Additionally, proteins responsible for physiological cell activity – guanine methyl transferase, aspartyl/asparaginyl beta-hydroxylase and GTP-binding protein, were identified and found to be more abundant in allantochorion of FMR mares than in controls.

Implications

  • The research confirms that FMR in mares is characterized by differences in protein pattern, which suggests that the condition including these protein alterations could lead to complications in the delivery process.
  • The research provides a foundation for further qualitative and quantitative studies to deepen our understanding of the mechanisms of the retention of foetal membranes in mares.

Cite This Article

APA
Wawrzykowski J, Rapacz-Leonard A, Wiącek D, Kankofer M, Janowski T. (2019). The preliminary studies on protein profile in retained and not retained foetal membranes in heavy draft mares. Reprod Domest Anim, 54(12), 1543-1551. https://doi.org/10.1111/rda.13561

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 54
Issue: 12
Pages: 1543-1551

Researcher Affiliations

Wawrzykowski, Jacek
  • Department of Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
Rapacz-Leonard, Anna
  • Department of Animal Reproduction with Clinic, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Wiącek, Dariusz
  • Institute of Agrophysics, Polish Academy of Sciences, Lublin, Poland.
Kankofer, Marta
  • Department of Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
Janowski, Tomasz
  • Department of Animal Reproduction with Clinic, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.

MeSH Terms

  • Animals
  • Endometrium / metabolism
  • Extraembryonic Membranes / metabolism
  • Female
  • Horses
  • Placenta / pathology
  • Placenta, Retained / metabolism
  • Placenta, Retained / veterinary
  • Pregnancy
  • Proteins / metabolism
  • Proteome / metabolism

Grant Funding

  • Statutory Activity of University of Life Sciences in Lublin

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Citations

This article has been cited 1 times.
  1. Wang X, Liu R, Chen Z, Zhang R, Mei Y, Miao X, Bai X, Dong Y. Combining Transcriptomics and Proteomics to Screen Candidate Genes Related to Bovine Birth Weight. Animals (Basel) 2024 Sep 23;14(18).
    doi: 10.3390/ani14182751pubmed: 39335340google scholar: lookup
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