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Equine veterinary journal2016; 49(4); 532-538; doi: 10.1111/evj.12629

The preventive effects of two nutraceuticals on experimentally induced acute synovitis.

Abstract: Nutraceuticals are often used in the management of equine osteoarthritis, but scientific evidence of their efficacy is lacking. Objective: To study the preventive effects of two new nutraceuticals after the experimental induction of synovitis in comparison with positive and negative control treatments. Methods: Blinded, controlled, randomised experiment. Methods: Twenty-four healthy Standardbred horses were randomly allocated to supplement AT (multi-ingredient, 28 days), supplement HP (collagen hydrolysate, 60 days), meloxicam (4 days) or placebo (60 days). Synovitis was induced in the right intercarpal joint by intra-articular injection of 0.5 ng lipopolysaccharide (LPS) of Escherichia coli while treatments were continued. Blood and synovial fluid were sampled before treatment, immediately prior to LPS injection, and at 8, 24 and 48 h post-injection. Synovial fluid samples were analysed for total nucleated cell count (TNCC), total protein (TP) and selected biomarkers (prostaglandin E [PGE ], interleukin-6 [IL-6], glycosaminoglycans [GAGs], type II collagen synthesis [CPII], matrix metalloproteinase [MMP]). Lameness was scored by visual examination and pressure plate analysis immediately prior to LPS injection, and at 8, 24 and 48 h post-injection. Clinical examinations were performed before treatment, immediately prior to LPS injection, at 2, 4 and 6 h post-injection, and then twice per day during the test period. Results: Before treatment and intra-articular challenge, there were no statistically significant differences among the treatment groups for any of the parameters. After intra-articular challenge, the placebo group showed significantly higher synovial fluid TP, TNCC and PGE compared with the meloxicam group, although the model did not induce a relevant amount of lameness. Both nutraceuticals resulted in significantly lower synovial fluid TP, TNCC and PGE compared with placebo. No statistical differences in IL-6, GAGs, CPII or MMPs were observed among treatment groups. No adverse effects were observed. Conclusions: Despite evidence of synovitis, lameness was too mild to detect. Conclusions: The preventive administration of these nutraceuticals showed anti-inflammatory effects in this validated synovitis model. Therefore, further studies of their clinical applicability are warranted.
© 2016 The Authors Equine Veterinary Journal published by John Wiley & Sons Ltd on behalf of EVJ Ltd.
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Publication Date: 2016-10-13 PubMed ID: 27554764PubMed Central: PMC5484312DOI: 10.1111/evj.12629Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explored the preventative impacts of two nutraceuticals on synovitis, a form of joint inflammation, in horses. Using an experimental model, it was found that the nutraceuticals seemed to exhibit anti-inflammatory effects.

Objective and Methodology

The study aimed to:

  • Evaluate the preventive effects of newly derived nutraceuticals on induced synovitis, benchmarking them against positive and negative control treatments.

The researchers conducted a blind, randomized, controlled experiment using 24 healthy Standardbred horses. The horses were divided into four groups, three of which received different treatments (AT supplement, HP supplement or meloxicam) and one group received a placebo.

These treatments aimed to test their impacts on synovitis, an inflammation in the joints, which was triggered in the right intercarpal joint of each horse by an injection of Escherichia coli lipopolysaccharide (LPS). Throughout the study duration, several variables like blood and synovial fluid, and lameness were monitored at different time points.

Results of Study

The study found:

  • Before the treatment and intra-articular challenge (injection of LPS to induce synovitis), there were no significant differences among groups with regard to any parameters under observation.
  • Following the injection, horses that received the placebo showed higher total protein (TP), total nucleated cell count (TNCC), and prostaglandin E (PGE) in the synovial fluid compared to those who received meloxicam.
  • Horses receiving nutraceuticals resulted in significantly lower TP, TNCC, and PGE in the synovial fluid as compared to placebo.
  • No significant differences noticed in interleukin-6, glycosaminoglycans, type II collagen synthesis or matrix metalloproteinase among different treatment groups.
  • All treatments could be administered without harmful effects.

Conclusion

In conclusion:

  • The study demonstrated that although there was evident synovitis, the induced lameness was too mild to detect. Therefore, the researchers conclude there’s room for further investigations using a more substantial model that captures clinically significant outcomes, like lameness.
  • The nutraceuticals under examination seemed to display anti-inflammatory effects on induced synovitis, laying the groundwork for future research to gauge their potential application clinically.

Cite This Article

APA
van de Water E, Oosterlinck M, Dumoulin M, Korthagen NM, van Weeren PR, van den Broek J, Everts H, Pille F, van Doorn DA. (2016). The preventive effects of two nutraceuticals on experimentally induced acute synovitis. Equine Vet J, 49(4), 532-538. https://doi.org/10.1111/evj.12629

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 49
Issue: 4
Pages: 532-538

Researcher Affiliations

van de Water, E
  • Department of Surgery and Anaesthesiology, Faculty of Veterinary Medicine, Ghent University, Belgium.
Oosterlinck, M
  • Department of Surgery and Anaesthesiology, Faculty of Veterinary Medicine, Ghent University, Belgium.
Dumoulin, M
  • Department of Surgery and Anaesthesiology, Faculty of Veterinary Medicine, Ghent University, Belgium.
Korthagen, N M
  • Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
van Weeren, P R
  • Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
van den Broek, J
  • Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
Everts, H
  • Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
Pille, F
  • Department of Surgery and Anaesthesiology, Faculty of Veterinary Medicine, Ghent University, Belgium.
van Doorn, D A
  • Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
  • Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
  • Equivado, Equine Nutrition Consultancy, Utrecht, The Netherlands.

MeSH Terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology
  • Collagen / chemistry
  • Dietary Supplements
  • Horse Diseases / prevention & control
  • Horses
  • Interleukin-6
  • Meloxicam
  • Protein Hydrolysates / pharmacology
  • Synovial Fluid / chemistry
  • Synovitis / prevention & control
  • Synovitis / veterinary
  • Thiazines / pharmacology
  • Thiazoles / pharmacology

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